A5090580282- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- Planarian Biology and Electrostimulation
- Genomics and Chromatin Dynamics
- Marine Ecology and Invasive Species
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Neuroendocrine regulation and behavior
- Drug Transport and Resistance Mechanisms
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Growth Hormone and Insulin-like Growth Factors
- Liver Disease and Transplantation
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Medical Imaging Techniques and Applications
- Reproductive Physiology in Livestock
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Veterinary Medicine and Surgery
- Electrolyte and hormonal disorders
- MXene and MAX Phase Materials
- Cancer Mechanisms and Therapy
- Orthopedic Infections and Treatments
Yale University
2016-2025
Yale Cancer Center
2023-2025
Whitney Museum of American Art
2022-2024
Whitehead Institute for Biomedical Research
2014-2016
Hubrecht Institute for Developmental Biology and Stem Cell Research
2005-2014
Howard Hughes Medical Institute
2014
Massachusetts Institute of Technology
2014
Utrecht University
1988-2009
National Institute for Public Health and the Environment
2005
Laboratoire de Biochimie
1988
RNA interference (RNAi)–related pathways affect gene activity by sequence-specific recruitment of Ago proteins to mRNA target molecules. The sequence specificity this process stems from small (sRNA) co-factors bound the protein. Stability sRNA molecules in some is part regulated Hen1-mediated methylation their 3′ ends. Here we describe effects Caenorhabditis elegans HEN1 RNA–methyl-transferase homolog, HENN-1, on different RNAi nematode. We reveal differential HENN-1 two that are known...
Protection of genomes against invasion by repetitive sequences, such as transposons, viruses, and transgenes, involves strong selective silencing these sequences. During a trans effect ("cosuppression") occurs that results in cognate endogenous genes. Here we report RNA interference (RNAi) screens performed to catalog genes required for cosuppression the Caenorhabditis elegans germline. We find factors with putative role chromatin remodeling involved RNAi. Together molecular data also...
We have shown recently that in the dog progestin administration results mammary production of immunoreactive growth hormone (GH). At present we demonstrate expression gene encoding GH gland dogs and cats using reverse-transcriptase PCR. mRNA was found great majority normal tissues as well benign malignant tumors associated with presence cryostat sections. The PCR product proved to be identical pituitary. highest levels were after prolonged treatment progestins. In carcinomas also...
The advent of sexual reproduction and the evolution a dedicated germline in multicellular organisms are critical landmarks eukaryotic evolution. We report an ancient family GCNA (germ cell nuclear antigen) proteins that arose earliest eukaryotes, feature rapidly evolving intrinsically disordered region (IDR). Phylogenetic analysis reveals emerged before major lineages diverged; predates origin by billion years. Gcna gene expression is enriched reproductive cells across eukarya – either just...
Optimized flowering time is an important trait that ensures successful plant adaptation and crop productivity. SOC1-like genes encode MADS transcription factors, which are known to play roles in control many plants. This includes the best-characterized eudicot model Arabidopsis thaliana (Arabidopsis), where SOC1 promotes functions as a floral integrator gene integrating signals from different flowering-time regulatory pathways. Medicago truncatula (Medicago) temperate reference legume with...
PIWI proteins are known as mediators of transposon silencing in animal germlines but also found adult pluripotent stem cells highly regenerative animals, where they essential for regeneration. Study the nuclear protein SMEDWI-2 planarian somatic cell system reveals an intricate interplay between transposons and differentiation which a subset is inevitably activated during differentiation, required to regain control. Absence leads tissue-specific derepression related cell-type-specific...
Many highly regenerative organisms maintain adult pluripotent stem cells throughout their life, but how the long-term maintenance of pluripotency is accomplished unclear. To decipher regulatory logic cells, we analyzed chromatin organization cell genes in planarian Schmidtea mediterranea . We identify a special state genes, which distinct from that tissue-specific and resembles constitutive genes. Where promoters have detectable transcription factor binding sites, cell–specific instead...
The oncomutation lysine 27-to-methionine in histone H3 (H3K27M) is frequently identified tumors of patients with diffuse midline glioma-H3K27 altered (DMG-H3K27a). H3K27M inhibits the deposition mark H3K27me3, which affects maintenance transcriptional programs and cell identity. Cells expressing are also characterized by defects genome integrity, but mechanisms linking expression oncohistone to DNA damage remain mostly unknown. In this study, we demonstrate that H3.1K27M model plant...
Changes in plasma variables as a result of liver damage induced by ethylene glycol (group A) or D-galactosamine B) and muscle doxycycline were compared. Plasma bile acid concentration was both specific sensitive indicator disease. Another specific, but less disease 7-GT. AS AT activity the most liver, not since increased ASAT activities also seen during ALAT slightly more to than 7-GT, being after damage. GLDH only extensive necrosis. AP no value for detecting pigeon. CK injury, though ALAT,...
Summary Tissue enzyme profiles of heart, liver, pectoral muscle, quadriceps duodenum, kidney and brain from racing pigeons were established. The enzymes alanine aminotransferase (ALAT), aspartate (ASAT), glutamate dehydrogenase (GLDH), lactate (LDH), gamma glutamyltransferase (y‐GT), alkaline phosphatase (AP), creatine kinase (CK). Elimination half‐lives (tyß) certain also determined. mean values (± SD) were: ASAT, 7.66 ± 1.55 (liver) 6.51 0.83 (muscle); ALAT, 15.69 + 1.70 11.99 1.32 LDH,...
Abstract Background The three-dimensional nuclear arrangement of chromatin impacts many cellular processes operating at the DNA level in animal and plant systems. Chromatin organization is a dynamic process that can be affected by biotic abiotic stresses. Three-dimensional imaging technology allows to follow these changes, but only few semi-automated processing methods currently exist for quantitative analysis 3D organization. Results We present an automated method, Nuclear Object DetectionJ...
Abstract Much insight has been gained on how stem cells maintain genomic integrity, but less attention paid to they their transcriptome. Here, we report that the PIWI protein SMEDWI-1 plays a role in filtering of dysfunctional transcripts from transcriptome planarian cells. accomplishes this through association with ribosomes during pioneer round translation, and processing poorly translated into piRNAs. This results removal such cytoplasmic pool at same time creates dynamic small RNAs for...
PIWI-interacting RNAs (piRNAs) play a crucial role in safeguarding genome integrity by silencing mobile genetic elements. From flies to human, piRNAs originate from long single-stranded precursors encoded genomic piRNA clusters. How clusters form adapt novel invaders and evolve maintain protection, remain key outstanding questions. Here, we generated roadmap of across seven species that highlights both similarities variations. In mammals, identified transcriptional readthrough as mechanism...