Abstract Chikungunya virus (CHIKV) has recently re‐emerged causing millions of infections in countries around the Indian Ocean. While CHIKV a broad host cell range and productively infects number different types, macrophages have been identified as potential viral reservoir serving to increase duration symptoms. To date no interacting protein characterized this study sought identify binding proteins expressed on target membranes. Two‐dimensional overlay prohibitin (PHB) microglial protein....

Abstract Chikungunya virus (CHIKV) is a re-emerging mosquito-borne alphavirus that has recently engendered large epidemics around the world. There no specific antiviral for treatment of patients infected with CHIKV and development compounds significant anti-CHIKV activity can be further developed to practical therapy urgently required. Andrographolide derived from Andrographis paniculata , herb traditionally used treat number conditions including infections. This study sought determine...

This work is aimed to formulate and analyze a mathematical modeling, SEIR model, for COVID-19 with the main parameters of vaccination rate, effectiveness prophylactic therapeutic vaccines. Global local stability model are investigated also numerical simulation. Local equilibrium points classified. A Lyapunov function constructed global disease-free equilibrium. The simulation part based on two situations, US India. In circumstance, result shows that rate 0.1% per day population at least 20%...

Abstract Melatonin controls many physiological functions including regulation of the circadian rhythm and clearance free radicals neuroprotection. Importantly, melatonin levels strongly decrease as we age patients with Alzheimer's disease ( AD ) display lower than age‐matched controls. Several studies have reported that can reduce aggregation toxicity amyloid‐ β peptides are produced from ‐amyloid precursor protein APP ). However, whether directly regulate ‐cleaving proteases (‘secretases’)...

Japanese encephalitis virus (JEV) a mosquito-borne flavivirus is major cause of viral in Asia. While the principle target cells for JEV central nervous system are believed to be neurons, microglia activated response and have been proposed act as long lasting reservoir. Viral attachment host cell first step entry process critical mediator tissue tropism. This study sought identify molecules associated with microglial cells. Virus overlay protein-binding assay (VOPBA) liquid...

The mosquito transmitted Dengue virus (DENV) remains a significant public health problem in many tropical and subtropical countries. Increasing evidence has suggested that during the infection process cellular lipids play important roles at several stages of replication cycle. This study sought to characterize changes lipid metabolism gene expression investigated role one enzyme, fatty acid synthase, DENV infection. Transcriptional profiles genes associated with were evaluated by real-time...

Andrographolide is a major bioactive constituent of Andrographis paniculata that has been shown in vitro to have antiviral activity against number viruses, including the mosquito transmitted dengue virus (DENV). However, how andrographolide exerts an anti-DENV effect remains unclear. This study therefore sought further understand mechanism action inhibiting DENV infection liver cells using proteomic based approach. Both 1 dimension (D) and 2D proteome systems were used. Initial data was...

Multidrug-resistant Acinetobacter baumannii (MDR A. baumannii) is one of the ESKAPE pathogens that restricts available treatment options. MDR responsible for a dramatic increase in case numbers wide variety infections, including skin and soft tissue infections (SSTIs), resulting pyoderma, surgical debridement, necrotizing fasciitis. To investigate an alternative medical SSTIs, broad range lytic phage, vB _AbP_ABWU2101 (phage vABWU2101), lysing associated SSTIs was isolated biological aspects...

Chikungunya virus (CHIKV) is a re-emerging mosquito-borne alphavirus that recently caused large epidemics in islands in, and countries around, the Indian Ocean. There currently no specific drug for therapeutic treatment or use as prophylactic agent against infection commercially available vaccine. Prohibitin has been identified receptor protein used by chikungunya to enter mammalian cells. Recently, synthetic sulfonyl amidines flavaglines (FLs), class of naturally occurring plant compounds...

Abstract Extended spectrum β lactamase-producing Klebsiella pneumoniae (ESBL-KP) is being reported with high morbidity and mortality rates considered as the highest priority for new antimicrobial strategies. To develop an alternative agent, phage KP1801 broad lytic activity was isolated. The genome of double stranded DNA 49,835 base pairs, a GC content 50.26%. There were 75 putative open reading frames. Phage classified in order Caudovirales , belonging to Siphoviridae family. About 323...

Abstract The emergence of carbapenem-resistant Acinetobacter baumannii (CRAB) has been increasingly reported, leading to greater challenges in treating infections. With the development phage therapy and phage-antibiotic combinations, it is promising improve treatment bacterial In present study, a novel vB_AbaP_WU2001 (vWU2001) phage-specific CRAB with genome 40,792 bp was isolated. Genomic analysis disclosed that belongs Autographiviridae family order Caudovirales . Phage vWU2001 had broad...

Multidrug-resistant (MDR) strains of

Chickens are the primary reservoirs of Campylobacter spp., mainly C. jejuni and coli, that cause human bacterial gastrointestinal infections. However, genomic characteristics antimicrobial resistance spp. in low- to middle-income countries need more comprehensive exploration. This study aimed characterize 21 5 coli isolates from commercial broilers native chickens using whole genome sequencing compare them 28 reference sequences. Among 26 isolates, 13 sequence types (ST) were identified ST...

Purpose. Extensively drug-resistant (XDR) strains of Acinetobacter baumannii are being reported worldwide, and they associated with high morbidity mortality rates. These considered to be the highest priority for development new antibacterial agents. Therefore, we aimed develop an effective alternative antimicrobial agent. Methodology. Bacteriophages (phages) were enriched recovered from a hospital waste water sample after activated sludge treatment. The biological characteristics therapeutic...

A green, simple, and rapid synthesis of gold nanoparticles using plant extract, Cryptolepis buchanani Roem. Schult, their applications are first described in this paper. The formation was visually observed by the appearance a ruby red color, which further indicated an absorption peak at 530 nm UV-Vis spectroscopy. Optimization reaction parameters for also investigated. Various analytical techniques were employed as part process characterizing resulting nanoparticles. Fourier transform...

Capsular polysaccharides enable clinically important clones of Klebsiella pneumoniae to cause severe systemic infections in susceptible hosts. Phage-encoded capsule depolymerases have the potential provide an alternative treatment paradigm patients when multiple drug resistance has eroded efficacy conventional antibiotic chemotherapy. An investigation 164 K . from intensive care Thailand revealed a large number distinct types low abundance but four (K2, K51, K1, K10) with frequency at least...

Background/Objectives: The significant outbreak of multidrug-resistant Klebsiella pneumoniae has emerged as a primary global concern associated with high morbidity and mortality rates. Certain strains K. are highly resistant to most antibiotics available in clinical practice, exacerbating the challenge bacterial infections. Methods: Phage vB_KpnP_PW7 (vKPPW7) was isolated characterized. Its morphology, stability, adsorption rate, one-step growth curve, lytic activity, whole-genome sequence...

Novel antimicrobial agents are urgently needed to combat the global threat of resistance. Actinobacteria remarkable producers bioactive compounds, which crucial for discovery novel drugs In current study, bioactivity, phylogenetic analysis, and a dereplication approach were employed quickly analyze metabolomic profiles selected strains aid in selection strain prioritization. The aim this study was screen Thai actinobacteria isolated from mangrove ecosystem antibacterial activities. Taxonomic...

Multidrug-resistant Acinetobacter baumannii (MDR A. baumannii) is an emerging pathogen in the ESKAPE group. The global burden of antimicrobial resistance has led to renewed interest alternative treatment strategies, including phage therapy. This study isolated and characterized a vB_AbaM_ ABPW7 (vABPW7) specific MDR baumannii. Morphological analysis showed that vABPW7 belongs Myoviridae family. Genome DNA genome consists 148,647 bp member Phapecoctavirus genus order Caudovirales. A short...