A5003385022- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Immunotherapy and Immune Responses
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Glycosylation and Glycoproteins Research
- Sepsis Diagnosis and Treatment
- Bacillus and Francisella bacterial research
- Multiple Myeloma Research and Treatments
- CAR-T cell therapy research
- Clostridium difficile and Clostridium perfringens research
- Monoclonal and Polyclonal Antibodies Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Influenza Virus Research Studies
- Bacterial biofilms and quorum sensing
- Inhalation and Respiratory Drug Delivery
- Nosocomial Infections in ICU
- Thermal Regulation in Medicine
- S100 Proteins and Annexins
- Galectins and Cancer Biology
- Bacterial Identification and Susceptibility Testing
University of Maryland, Baltimore
2016-2025
Commonwealth Medical College
2024
AstraZeneca (United States)
2018
University of Maryland Medical Center
2002-2017
Walter Reed Army Institute of Research
1991-2015
University of Pennsylvania
2014
Oncovir (United States)
2014
University of Baltimore
2013
National Cancer Institute
2012
National Institutes of Health
1983-2012
Objectives To determine the safety, pharmacokinetics, and activity of an anti-tumor necrosis factor (TNF)-α monoclonal antibody in severe sepsis. Design Open-label, prospective, phase II multicenter trial with escalating doses a murine (CB0006). Setting Twelve academic medical center intensive care units United States Europe. Patients Eighty patients sepsis or septic shock who received standard supportive antimicrobial therapy addition to anti-TNF antibody. Interventions were treated...
We used monoclonal antibodies and flow cytometry to study the expression of receptors for complement fragments C3bi (CR3) C3b (CR1) on human polymorphonuclear neutrophil leukocytes (PMN). Expression both was minimal cells stained in anticoagulated whole blood incubated at 0 degree or 37 degrees C. PMN isolated with Percoll density gradients held C also had only receptors. With cells, however, a spontaneous increase occurred upon warming This did not represent complete either receptor since...
Klebsiella pneumoniae causes community- and healthcare-associated infections in children adults. Globally 2019, an estimated 1.27 million (95% Uncertainty Interval [UI]: 0.91-1.71) 4.95 UI: 3.62-6.57) deaths were attributed to associated with bacterial antimicrobial resistance (AMR), respectively. K. was the second leading pathogen AMR resistant bacteria. Furthermore, rise of both hospital-acquired is a concern for neonates infants who are at high risk invasive disease. There limited...
Although Escherichia coli strains possessing the K1 capsule are predominant among isolates from neonatal E. meningitis and most of these associated with a limited number 0 lipopolysaccharide (LPS) types, basis this association certain antigens is not clear. The present study examined in experimental bacteremia newborn adult rats whether or and/or O-LPS antigen critical determinants development meningitis. Rats received subcutaneously at strain (018+K1+) mutants lacking either (018+K1-)...
ABSTRACT Fever, a nonspecific acute-phase response, has been associated with improved survival and shortened disease duration in infections, but the mechanisms of these beneficial responses are poorly understood. We previously reported that increasing core temperature bacterial endotoxin (LPS)-challenged mice to normal febrile range modified expression tumor necrosis factor alpha (TNF-α), interleukin 1β (IL-1β), IL-6, three cytokines critical mounting an initial defense against microbial...
Tumor necrosis factor/cachectin (TNF/C) is the principal mediator of bacterial endotoxin-induced shock and death. We found that C3H/HeJ mouse, which less able to produce TNF/C in response endotoxin, has a 1,000-fold greater susceptibility lethal infection with Escherichia coli than TNF-responsive congenic C3H/HeN. This surprising finding suggested this peptide may also be involved host protection. To test hypothesis we pretreated mouse combination recombinant murine TNF/C-alpha IL-1 alpha....
Respiratory syncytial virus infection of the upper airways may progress to fatal pneumonia in hematopoietic cell transplant recipients. The safety and efficacy aerosolized ribavirin preventing disease progression is unknown.In a multicenter prospective trial, recipients with respiratory were randomized receive (2 g 3 times daily) or supportive care for 10 days. primary end point was radiographically proven pneumonia. Secondary points included virologically pneumonia, viral load changes,...
Levels of antibody to toxin A Pseudomonas aeruginosa were determined by a solidphase radioimmunoassay. Mean (±SEM) peak levels IgG in 24 normal soldiers 2.6 ± 0.5 μg/ml, while mean 12patients colonized with and 13 patients infected sites other than the blood A-producing strains 16.7 7.0 17.1 4.4 respectively. 52 pseudomonas bacteremia, those surviving dying bacteremia due had 25.8 5.5 4.6 2.0 The antitoxin response sequential bacteremic sera began shortly after onset decreased gradually, but...
Myeloma-directed cellular immune responses after autologous stem cell transplantation (ASCT) may reduce relapse rates. We studied whether coinjecting the TLR-3 agonist and vaccine adjuvant Poly-ICLC with a MAGE-A3 peptide was safe would elicit high frequency of vaccine-directed when combined vaccine-primed costimulated T cells.In phase II clinical trial (NCT01245673), we evaluated safety activity ex vivo expanded cells primed in using multipeptide (compound GL-0817) (Hiltonol), granulocyte...
The IFN family of cytokines operates a frontline defense against pathogens and neoplastic cells in vivo by controlling the expression several genes. death-associated protein kinase 1 (DAPK1), an IFN-γ–induced enzyme, controls cell cycle, apoptosis, autophagy, tumor metastasis, its is frequently down-regulated number human tumors. Although biochemical action DAPK1 well understood, mechanisms that regulate are unclear. Previously, we have shown transcription factor C/EBP-β required for basal ....
Klebsiella pneumoniae is a common cause of sepsis and particularly associated with healthcare-associated infections. New strategies are needed to prevent or treat infections due the emergence multi-drug resistant K. pneumoniae. The goal this study was determine global diversity distribution O (lipopolysaccharide) K (capsular polysaccharide) antigens on large (>500) collection strains isolated from blood inform vaccine development efforts. A total 645 isolates were collected patients...
Abstract Background Pseudomonas aeruginosa is an opportunistic pathogen that causes a wide range of acute and chronic infections frequently associated with healthcare-associated infections. Because its ability to rapidly acquire resistance antibiotics, P. are difficult treat. Alternative strategies, such as vaccine, needed prevent . We collected total 413 isolates from the blood cerebrospinal fluid patients 10 countries located on 4 continents during 2005–2017 characterized these inform...
ABSTRACT When given in the presence of gamma interferon (IFN-γ), otherwise nontoxic doses lipopolysaccharide (LPS or endotoxin) become highly lethal for mice. The mechanisms this synergistic toxicity are not known. We considered possibility that an interaction between LPS-induced NF-κB and IFN-γ-induced JAK-STAT pathways at pretranscriptional level may enhance signals. To test hypothesis, we incubated murine macrophage RAW 264.7 cells with IFN-γ 2 h before addition different LPS. Consistent...
Journal Article Inflammatory Cytokines Produced in Response to Experimental Human Gonorrhea Get access Kyle H. Ramsey, Ramsey Department of Bacterial Diseases, Division Communicable Disease and Immunology, Walter Reed Army Institute Research, Washington, DC Reprints or correspondence (current address): Dr. Ramsey. Dept. Microbiology, Midwestern University, 555 31st St., Downers Grove, IL 60515. Search for other works by this author on: Oxford Academic PubMed Google Scholar Herman Schneider,...
The polysaccharide capsule of Klebsiella pneumoniae is an important virulence factor that confers resistance to phagocytosis. treatment encapsulated bacteria with salicylate inhibit expression was found enhance the phagocytosis by human neutrophils only in presence cell surface-specific antibodies. Both type-specific rabbit antisera and anticapsular hyperimmune globulin were employed as opsonins. Salicylate significantly enhanced homologous, but not heterologous, whole-cell antisera....
Abstract MUC1 (MUC1 in human and Muc1 nonhumans) is a membrane-tethered mucin that interacts with Pseudomonas aeruginosa (PA) through flagellin. In this study, we compared PA pulmonary clearance proinflammatory responses by Muc1−/− mice Muc1+/+ littermates following intranasal instillation of or Compared mice, showed increased clearance, greater airway recruitment neutrophils, higher levels TNF-α KC bronchoalveolar lavage fluid, media flagellin-stimulated alveolar macrophages, tracheal...
Bacillus anthracis exotoxins mediate most of the symptomatology severe anthrax. In addition to a clinical syndrome reminiscent septic shock, which may be mediated by cytokines produced macrophages stimulated with lethal toxin, infected patients show profound edema at sites infection. Edema is toxin (ET), comprises binding molecule, protective antigen, and an active moiety, factor, possesses intrinsic adenylyl cyclase activity. Intracellular cyclic AMP (cAMP) regulates production several that...
We examined 534 clinical isolates of Escherichia coli for sensitivity to rough lipopolysaccharide-specific and K1-specific phages. Twenty-eight percent bacteremic were sensitive rough-specific Forty-two these strains, against only 20% insensitive phages, had K1 capsule (P less than 0.001). K1-positive strains usually resistant phagocytic killing, whereas lacking the more likely be killed regardless capsular type. Eighty-two typable with O-specific, 57% K-specific, 74% H-specific antisera....
Bacterial lipopolysaccharide (LPS) is a key mediator in the vascular leak syndromes associated with Gram-negative bacterial infections. LPS opens paracellular pathway pulmonary endothelia through protein tyrosine phosphorylation. We now have identified protein-tyrosine kinases (PTKs) and their substrates required for LPS-induced phosphorylation opening of human lung microvascular endothelial cells (HMVEC-Ls). disrupted barrier integrity dose- time-dependent manner, prior broad spectrum PTK...