A5083767492- Selenium in Biological Systems
- Trace Elements in Health
- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Bone health and treatments
- Genetic Associations and Epidemiology
- Pregnancy and Medication Impact
- Spondyloarthritis Studies and Treatments
- Gout, Hyperuricemia, Uric Acid
- Bone health and osteoporosis research
- Folate and B Vitamins Research
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Heavy Metal Exposure and Toxicity
- Thallium and Germanium Studies
- Spine and Intervertebral Disc Pathology
- Bioinformatics and Genomic Networks
- Glutathione Transferases and Polymorphisms
- Mycotoxins in Agriculture and Food
- Genetics and Neurodevelopmental Disorders
- Rheumatoid Arthritis Research and Therapies
- Connective Tissue Growth Factor Research
- Mitochondrial Function and Pathology
- Circular RNAs in diseases
- Inflammatory mediators and NSAID effects
Xi'an Jiaotong University
2016-2025
National Health and Family Planning Commission
2016-2025
Xijing Hospital
2016-2025
Second Hospital of Yichang
2025
Air Force Medical University
2016-2025
China Three Gorges University
2024-2025
Fujian Agriculture and Forestry University
2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2024
Chongqing Medical University
2024
Xian Yang Central Hospital
2024
Objectives Single-cell and spatial transcriptomics analysis of human knee articular cartilage tissue to present a comprehensive transcriptome landscape osteoarthritis (OA)-critical cell populations. Methods RNA sequencing spatially resolved transcriptomic technology have been applied characterise the cellular heterogeneity which were collected from 8 OA donors, 3 non-OA control total 19 samples. The novel chondrocyte population marker genes interest validated by immunohistochemistry...
Chondrocytes are the key target cells of cartilage degeneration that occurs in Kashin-Beck disease (KBD) and osteoarthritis (OA). However, heterogeneity articular cell types present KBD OA patients healthy controls is still unknown, which has prevented study pathophysiology mechanisms underlying roles different populations chondrocytes processes leading to OA. Here, we aimed identify transcriptional programmes all major with KBD, markers discriminate among these three groups. Single-cell RNA...
We sought to determine whether transplanted allogeneic bone mesenchymal stem cells can survive and increase the amount of proteoglycans in intervertebral discs. used rabbit disc as a model, creating three groups: an uninjected control group, group injected with saline, 1 × 105 containing trace marker gene LacZ from young rabbits. At 1, 3, 6 months, X-gal staining DNA-polymerase chain reaction neomycin-resistant were ascertain cell location. Reverse transcription-polymerase enzyme-linked...
To investigate the differences in gene expression profiles of adult articular cartilage from patients with Kashin-Beck disease (KBD) versus those primary knee osteoarthritis (OA).The messenger RNA KBD, diagnosed according to clinical criteria for KBD China, were compared OA, Western Ontario and McMaster Universities OA Index. Total was isolated separately 4 pairs samples, evaluated by Agilent 4x44k Whole Human Genome density oligonucleotide microarray analysis. The data selected transcripts...
Intervertebral disc degeneration (IDD) is associated with lower back pain and a global burden severe healthcare socioeconomic consequences. However, the underlying mechanisms of IDD remain largely unelucidated. Accumulating evidence has indicasted that newly defined gene regulators, microRNAs (miRNAs), play vital role in neurodegenerative, pathophysiological certain reproductive disorders. To characterize differential miRNA expression profiles between spinal cord injury, specimens from 3...
T-2 toxin, a mycotoxin produced by Fusarium species, has been shown to cause diverse toxic effects in animals and is also possible pathogenic factor of Kashin–Beck disease (KBD). The role mitochondria KBD recognized our recent research. aim this study was evaluate the toxin-induced human chondrocytes apoptosis understand pathogenesis KBD. toxin decreased viabilities concentration- time-dependent manners. Exposure can reduce activities mitochondrial complexes III, IV V, ΔΨm cellular ATP,...
Co-delivery of anti-cancer drugs is promising to improve the efficacy cancer treatment. This study was aiming investigate potential concurrent delivery resveratrol (RES) and docetaxel (DTX) via polymeric nanocarriers treat breast cancer. To this end, methoxyl poly(ethylene glycol)-poly(d,l-lactide) copolymer (mPEG-PDLA) prepared characterized using FTIR 1H NMR, their molecular weights were determined by GPC. Isobologram analysis combination index calculation performed find optimal ratio...
Abstract Mesenchymal stem cells (MSCs) delivered into the post-ischemic heart milieu have a low survival and retention rate, thus restricting cardioreparative efficacy of MSC-based therapy. Chronic ischemia results in metabolic reprogramming heart, but little is known about how these changes influence implanted MSCs. Here, we found that excessive branched-chain amino acid (BCAA) accumulation, signature seen was disadvantageous to cardioprotection intramyocardially injected Discovery-driven...
BACKGROUND: Myocardial mitochondrial dysfunction underpins the pathogenesis of heart failure (HF), yet therapeutic options to restore myocardial function are scarce. Epigenetic modifications DNA (mtDNA), such as methylation, play a pivotal role in modulating homeostasis. However, their involvement HF remains unclear. METHODS: Experimental models were established through continuous angiotensin II and phenylephrine (AngII/PE) infusion or prolonged ischemia/reperfusion injury. The landscape N 6...
A case-control study of 4180 subjects was carried.To explore the risk factors lumbar disc herniation in China.In China, along with economic development, people's living environment and working conditions have undergone some tremendous changes, such as accelerating life pace competition. In this situation, it is important to know whether changed or not. This study, including possible social psychological based on literature, attempted search new factor, therefore provide better prevention...
The relationship between T-2 toxin and deoxynivalenol (DON) the risk of Kashin-Beck disease is still controversial since it poorly known about their selectivity in cartilage damage. We aimed to compare cytotoxicity DON on cell lines representative types encountered vivo, including human chondrocytes (C28/I2), hepatic epithelial cells (L-02) tubular (HK-2). In addition, we determined distribution Sprague-Dawley (SD) rats after a single dose exposure. or decreased proliferation time-...