A5017740228- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Fibroblast Growth Factor Research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Circular RNAs in diseases
- Lung Cancer Treatments and Mutations
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Kruppel-like factors research
- Eosinophilic Disorders and Syndromes
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Infective Endocarditis Diagnosis and Management
- Brain Metastases and Treatment
- CRISPR and Genetic Engineering
- Bacterial Identification and Susceptibility Testing
- RNA modifications and cancer
- Signaling Pathways in Disease
- Antimicrobial Peptides and Activities
- Orthopedic Infections and Treatments
- Advanced Proteomics Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Infectious Diseases and Tuberculosis
University Medical Center Groningen
2017-2024
University of Groningen
2017-2024
Spanish National Cancer Research Centre
2016-2022
Centro de Investigación Biomédica en Red de Cáncer
2019-2022
Research Institute Hospital 12 de Octubre
2016-2018
Instituto de Biomedicina de Sevilla
2011-2014
Hospital Universitario Virgen Macarena
2007
Lung adenocarcinoma and chronic obstructive pulmonary disease (COPD) are diseases that share common aetiological factors (tobacco smoking) probable dysregulated pathways. MicroRNAs (miRNAs) play an essential role in regulating numerous physiological pathological processes. The purpose of this study was to assess global miRNA expression patterns patients with COPD and/or elucidate distinct regulatory networks involved the pathogenesis these two smoking-related diseases. Expression 381 miRNAs...
Abstract Whole-brain radiotherapy (WBRT) is the treatment backbone for many patients with brain metastasis; however, its efficacy in preventing disease progression and associated toxicity have questioned clinical impact of this approach emphasized need alternative treatments. Given limited therapeutic options available these poor understanding molecular mechanisms underlying resistance metastatic lesions to WBRT, we sought uncover actionable targets biomarkers that could help refine patient...
The availability of complete genome sequences and the definition essential gene sets were fundamental in start engineering era. In a recent study, redundant unnecessary genes systematically deleted from Gram-positive bacterium Bacillus subtilis, an industrial production host high-value secreted proteins. This culminated strain PG10, which lacks about 36% genome, thus representing most minimal chassis currently available. Here, we show that this "miniBacillus" has synthetic traits are...
IntroductionThere is substantial evidence for the oncogenic effects of fibroblast growth factor receptor 1 (FGFR1) in many types cancer, including lung but role this has not been addressed specifically adenocarcinoma.MethodsWe performed FGFR1 and EGFR overexpression co-overexpression assays adenocarcinoma inmortalized cell lines, we also carried out surrogate interaction assays. We monotherapy combination EGFR/FGFR inhibitor sensitivity vitro vivo line– patient-derived xenografts. determined...
The identification of new less invasive biomarkers is necessary to improve the detection and prognostic outcome respiratory pathological processes. measurement miRNA expression through techniques such as plasma serum have been suggested analysis several lung malignancies including cancer. These studies are assuming a common deregulated both in blood tissue. present study aimed obtain representative signatures bronchoalveolar cell fraction that could serve biomarker diseases. Ten patients...
The identification of new less invasive biomarkers is necessary to improve the detection and prognostic outcome respiratory pathological processes. measurement miRNA expression through techniques such as plasma serum have been suggested analysis several lung malignancies including cancer. These studies are assuming a common deregulated both in blood tissue. present study aimed obtain representative signatures bronchoalveolar cell fraction that could serve biomarker diseases. Ten patients...
Abstract Positron emission tomography (PET) with fluorine-18-fluorodeoxyglucose ( 18 F-FDG) can be applied to detect infection and inflammation. However, it was so far not known what extent bacterial pathogens may contribute the PET signal. Therefore, we investigated whether clinical isolates of frequently encountered take up F-FDG in vitro , FDG inhibits growth as previously shown for 2-deoxy-glucose. 22 Gram-positive Gram-negative implicated fever inflammation were incubated uptake...
The high incidence and mortality of lung tumours is a major health problem. Therefore, the identification both biomarkers predicting efficacy for therapies in use novel efficacious therapeutic agents crucial to increase patient survival. MAP17 (PDZK1IP1) small membrane-bound protein whose upregulation reported as common feature from diverse histological origins. Furthermore, correlated with tumour progression.We assessed expression preclinical models, including cell lines patient-derived...
Abstract The scaffold protein spinophilin (Spn, PPP1R9B) is one of the regulatory subunits phosphatase‐1a (PP1), targeting it to distinct subcellular locations and its target. Loss Spn reduces PPP1CA levels, thereby maintaining higher levels phosphorylated pRb. This effect contributes an increase in p53 activity. However, absence p53, reduced tumourigenic properties cells. In addition, knockout mice have a lifespan, increased number tumours cellular proliferation some tissues, such as...
The Gram-positive bacterium Bacillus subtilis is extensively used in the industry for secretory production of proteins with commercial value. To further improve its performance, this microbe has been subject extensive genome engineering efforts, especially removal large genomic regions that are dispensable or even counterproductive. Here, we present genome-reduced B. strain IIG-Bs-27-39, which was obtained through systematic deletion mobile genetic elements, as well genes extracellular...
has been extensively used as a microbial cell factory for industrial enzymes due to its excellent capacities protein secretion and large-scale fermentation. This bacterium is also an attractive host biopharmaceutical production. However, the potential of this organism not fully utilized yet, mostly limited understanding critical rearrangements in membrane proteome upon high-level secretion. Recently, it was shown that bottlenecks heterologous can be resolved by genome minimization. Here, we...
The bacterial twin-arginine (Tat) pathway serves in the exclusive secretion of folded proteins with bound cofactors. While Tat pathways Gram-negative bacteria and chloroplast thylakoids consist conserved TatA, TatB TatC subunits, Bacillus species many other Gram-positive stand out for their minimalist nature core translocase being composed essential TatA subunits only. Here we addressed question whether minimal TatAyCy subtilis recruits additional cellular components that modulate its...
Genome engineering offers the possibility to create completely novel cell factories with enhanced properties for biotechnological applications. In recent years, genome minimization was extensively explored in Gram-positive bacterial factory Bacillus subtilis, where up 42% of encoding dispensable functions removed. Such studies showed that some strains minimized genomes gained beneficial features, especially secretory protein production. However, most minimal displayed growth defects. This...
Lung tumors represent a major health problem. In early stage NSCLC tumors, surgical resection is the preferred treatment, but 30-55% of patients will relapse within 5 years after surgery. Thus, identification prognostic biomarkers in patients, especially those which are therapeutically addressable, crucial to enhance survival these patients. We determined immunohistochemistry expression key proteins involved tumorigenesis and oncogenic signaling, p53, EGFR, pAKT pERK, correlated their level...
Bacterial resistance to antibiotics has become a serious health care problem. The spread of antibiotic genes between bacteria the same or different species is often mediated by process named conjugation, where donor cell transfers DNA recipient through connecting channel.
Vanishing white matter (VWM) is a childhood leukoencephalopathy with central hypomyelination, rarefaction, and cystic degeneration. Adult onset, variable phenotype, high frequency characterize Arg113His mutation caused by G338A polymorphism associated VWM. A patient trauma-associated clinical features compatible multiple sclerosis (MS), was homozygous for of eukaryotic translation initiation factor (eIF2B5). The authors checked cohort 101 MS patients, including 19 head/neck failed to find...
Abstract Background: Translational cancer research needs appropriated preclinical models to discover predictive biomarkers and evaluate precision therapeutic strategies. Conventional available have proven not been optimal this end, since cell lines based xenografts do reconstitute the complexity of human carry acquired mutations found in original tumor. For these reasons our aim was generate completely characterize a collection non-small lung (NSCLC) PDX useful for translational research....
Abstract Squamous cell lung cancer and adenocarcinoma are the most common subtypes of tumours. The search for cancer-directed treatments has increased need understanding molecular features either histological subtypes. aim this study was to identify transcriptional regulation differences due miRNA expression profiles between SCC adenocarcinoma. In work, a total 44 patients were evaluated assess correlation messenger RNA levels. We detected changes in 56 mRNAs as well 9 miRNAs Nearly 20%...
Abstract MicroRNAs (miRNAs) are post-transcriptional regulators involved in numerous biological and pathological processes including colorectal cancer (CRC). From a clinical perspective, several studies have identified groups of miRNAs with potential utility for early diagnosis or prognostic stratification CRC patients. However, very few evaluated the ability miRNA to predict response selected chemotherapy regimens. The aim our study was evaluate expression patterns cohort 78 patients...
Abstract The FGFR4-388Arg allele, where an arginine substitutes a glycine in the 388 codon of FGFR4 gene, has been associated to poorer patient outcome different tumor types. In lung cancer, variant related shorter overall survival adenocarcinoma (ADC) patients, while no relationship with squamous cell carcinoma prognosis (SCC) reported. Mechanistically, increased MAPK pathway activation and EMT prostate cancer vitro models. Furthermore, higher STAT3 signaling murine models breast cancer....