A5064732288- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Blood disorders and treatments
- Immune cells in cancer
- CAR-T cell therapy research
- S100 Proteins and Annexins
- Immunotherapy and Immune Responses
- Nitric Oxide and Endothelin Effects
- Neurotransmitter Receptor Influence on Behavior
- Cancer Immunotherapy and Biomarkers
- Graphene and Nanomaterials Applications
- Receptor Mechanisms and Signaling
- Nanoplatforms for cancer theranostics
- Pharmacological Effects and Assays
- Vasculitis and related conditions
- Asthma and respiratory diseases
- Neonatal and Maternal Infections
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Genetics and Neurodevelopmental Disorders
- Lipid Membrane Structure and Behavior
- Molecular Communication and Nanonetworks
- Advanced Nanomaterials in Catalysis
- Medicine and Dermatology Studies History
- Antifungal resistance and susceptibility
Centre for Human Drug Research
2022-2025
Leiden University
2022-2025
University of Göttingen
2018-2024
Universitätsmedizin Göttingen
2019-2024
Leiden University Medical Center
2023
Fraunhofer Institute for Microelectronic Circuits and Systems
2022
Biologie et Gestion des Risques en Agriculture
2020
University Medical Center
2020
Neutrophilic granulocytes are able to release their own DNA as neutrophil extracellular traps (NETs) capture and eliminate pathogens. expulsion (NETosis) has also been documented for other cells organisms, thus highlighting the evolutionary conservation of this process. Moreover, dysregulated NETosis implicated in many diseases, including cancer inflammatory disorders. During NETosis, neutrophils undergo dynamic dramatic alterations cellular well sub-cellular morphology whose biophysical...
Serotonin is an important neurotransmitter involved in various functions of the nervous, blood, and immune system. In general, detection small biomolecules such as serotonin real time with high spatial temporal resolution remains challenging conventional sensors methods. this work, we designed a near-infrared (nIR) fluorescent nanosensor (NIRSer) based on single-walled carbon nanotubes (SWCNTs) to image release from human blood platelets time. The consists nonbleaching SWCNT backbone, which...
The formation of Neutrophil Extracellular Traps (NETs) is an immune defense mechanism neutrophil granulocytes. Moreover, it also involved in the pathogenesis autoimmune, inflammatory and neoplastic diseases. For that reason, process NET subject intense ongoing research. In vitro approaches to quantify (NETosis) are commonly used involve stimulation with various activators such as phorbol 12-myristate 13-acetate (PMA), lipopolysaccharides (LPS) or calcium ionophores (CaI). However,...
Neutrophils are key effector cells of the innate immune system that respond to small signaling molecules, which coordinate and regulate responses. For a long time, similarities between neuronal have been discussed. Here, we show human neutrophils rapidly take up, package, traffic use catecholamine neurotransmitters such as dopamine or epinephrine via machinery known from neurons. Uptake release catecholamines well trafficking packaging into MPO/VMAT2-positive primary vesicles is visualized...
Integrins are transmembrane receptors that mediate cell-adhesion, signaling cascades and platelet-mediated blood clotting. Most integrins bind to the common short peptide Arg-Gly-Asp (RGD). The conformational freedom of RGD motif determines how strong which it binds. Here, we present a novel approach tune binding constants by confining motifs via noncovalent adsorption single-stranded DNA (ssDNA) anchors onto single-walled carbon nanotubes (SWCNTs). Semiconducting SWCNTs display fluorescence...
Neutrophils are the most abundant type of white blood cells. Upon stimulation, they able to decondense and release their chromatin as neutrophil extracellular traps (NETs). This process (NETosis) is part immune defense mechanisms but also plays an important role in many chronic inflammatory diseases such atherosclerosis, rheumatoid arthritis, diabetes, cancer. For this reason, much effort has been invested into understanding biochemical signaling pathways NETosis. However, impact mechanical...
Neutrophil Extracellular Traps (NETs) are produced by neutrophilic granulocytes and consist of decondensed chromatin decorated with antimicrobial peptides. They defend the organism against intruders released upon various stimuli including pathogens, mediators inflammation or chemical triggers. NET formation is also involved in inflammatory, cardiovascular, malignant diseases autoimmune disorders like rheumatoid arthritis, psoriasis systemic lupus erythematosus (SLE). In many SLE...
Bispecific antibodies (bsAbs) bridging CD3 on T-cells to tumor-associated antigens (TAA) tumor cells can direct T-cell immunity solid tumors. "Bystander killing", where targeting of TAA-positive also leads the eradication TAA-negative cells, may overcome TAA heterogeneity. While bystander activity activated, engineered has been shown be robust and wide-reaching, spatiotemporal aspects bsAb-mediated are unresolved. Here, we developed a model breast cancer tumoroids varying in HER2 expression...
Immune cells take up nanoscale materials and can be programmed to release it again, which has important implications for understanding cellular functions, biocompatibility as well biomedical applications.
Neutrophil extracellular traps (NETs) contribute to the pathophysiology of multiple inflammatory and autoimmune diseases. Targeting NETosis pathway has demonstrated significant therapeutic potency in various disease models. Here, we describe a first-in-class monoclonal antibody (CIT-013) with high affinity for citrullinated histones H2A H4, which inhibits reduces tissue NET burden
Neutrophils are key players of the immune system and possess an arsenal effector functions, including ability to form expel neutrophil extracellular traps (NETs) in a process termed NETosis. During NETosis, nuclear DNA/chromatin expands until it fills whole cell is released into space. NETs composed DNA decorated with histones, proteins, or peptides, NETosis implicated many diseases. Resolving structure nucleus great detail essential understand underlying processes, but so far,...
Abstract Neutrophils are the most abundant type of white blood cells. Upon stimulation, they able to decondense and release their chromatin as neutrophil extracellular traps (NETs). This process (NETosis) is part immune defense mechanisms but also plays an important role in many chronic inflammatory diseases such atherosclerosis, rheumatoid arthritis, diabetes cancer. For this reason, much effort has been invested into understanding biochemical signaling pathways NETosis. However, impact...
Elevated levels of peripheral blood and tumor tissue neutrophils are associated with poorer clinical response therapy resistance in melanoma. The underlying mechanism the role targeted is still not fully understood. Serum samples patients advanced melanoma were collected neutrophil-associated serum markers measured correlated to therapy. Blood from healthy donors isolated, their phenotypes, as well vitro functions, compared. In functional tests conducted through nonadherent cocultures cells....
<h3>Background</h3> Neutrophil extracellular traps (NETs) contribute to the pathophysiology of multiple inflammatory and autoimmune diseases. Targeting NETosis pathway has demonstrated significant therapeutic potency in various disease models (Chirivi <i>et al</i>., 2021). Here we describe a first class monoclonal antibody (CIT-013) with high affinity for citrullinated histones H2A H4 which inhibits reduces tissue NET burden <i>in vivo</i> anti-inflammatory consequences. CIT-013 is currently...
Abstract Transport and delivery of (nanoscale) materials are crucial for many applications in biomedicine. However, controlled uptake, transport triggered release such cargo remains challenging. In this study, we use human immune cells (neutrophilic granulocytes, neutrophils) program them to perform these tasks vitro. For purpose, let neutrophils phagocytose a nanoscale cargo. As an example, used DNA-functionalized single-walled carbon nanotubes (SWCNT) that fluoresce the near infrared (980...
Serotonin is an important neurotransmitter involved in various functions of the nervous, blood, and immune system. In general, detection small biomolecules such as serotonin real time with high spatial temporal resolution remains challenging conventional sensors methods. this work, we designed a near-infrared (nIR) fluorescent nanosensor (NIRSer) based on single-walled carbon nanotubes (SWCNTs) to image release from human blood platelets time. The consists nonbleaching SWCNT backbone, which...
Abstract Neutrophils are key players of the immune system and possess an arsenal effector functions, including ability to form expel neutrophil extracellular traps (NETs) in a process termed NETosis. During NETosis, nuclear DNA/chromatin expands until it fills whole cell is released into space. NETs composed DNA decorated with histones, proteins or peptides NETosis implicated many diseases. Resolving structure dynamics nucleus great detail essential understand underlying processes but so far...