A5008515664- Mesenchymal stem cell research
- Bone health and treatments
- Bone and Joint Diseases
- Oral Health Pathology and Treatment
- Cancer Diagnosis and Treatment
- Oral and gingival health research
- Periodontal Regeneration and Treatments
- Wound Healing and Treatments
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Oral and Maxillofacial Pathology
- HER2/EGFR in Cancer Research
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Dermatologic Treatments and Research
- Multiple Myeloma Research and Treatments
- Neurotransmitter Receptor Influence on Behavior
- Laser Applications in Dentistry and Medicine
- Reconstructive Surgery and Microvascular Techniques
- Neuroscience and Neuropharmacology Research
- Glycosylation and Glycoproteins Research
University of Pennsylvania
2015-2024
Hospital of the University of Pennsylvania
2014-2023
University of Pennsylvania Health System
2014-2023
Industrial University of Ho Chi Minh City
2023
The Ohio State University
2020
Chapter Arts Centre
2020
Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie
2020
Olivia Newton-John Cancer Wellness & Research Centre
2018
The University of Melbourne
2018
St Vincent's Hospital
2018
Epidermal growth factor (EGF) at 3 nM maximally inhibits the proliferation of A431 epidermoid carcinoma cells. We show that lower concentrations, in range 3-100 pM, EGF has a mitogenic effect on In presence 100 anti-EGF-receptor monoclonal IgG (designated 528), which cell and blocks greater than 95% binding, becomes for cells concentrations up to nM. These results suggest minor population high-affinity receptors may be involved stimulation proliferation. Saturation binding assays with...
Aside from the well-established self-renewal and multipotent differentiation properties, mesenchymal stem cells exhibit both immunomodulatory anti-inflammatory roles in several experimental autoimmune inflammatory diseases. In this study, we isolated a new population of human gingiva, tissue source easily accessible oral cavity, namely, gingiva-derived (GMSCs), which exhibited clonogenicity, self-renewal, capacities. Most importantly, GMSCs were capable functions, specifically suppressed...
Four mouse monoclonal antibodies specific for human epidermal growth factor (EGF) receptors have been prepared using EGF receptor protein from A431 epidermoid carcinoma cells as immunogen. We determined the effect of these on two known functions receptor: binding and tyrosine kinase. Three (225, 528, 579) are inhibitors binding, whereas fourth (455) does not compete but immunoprecipitates receptor. Inhibition is mixed competitive noncompetitive type. The three competing antagonists...
Mesenchymal stem cell (MSC)–derived exosome plays a central role in the cell-free therapeutics involving MSCs and contents can be customized under disease-associated microenvironments. However, optimal MSC-preconditioning to enhance its therapeutic potential is largely unknown. Here, we show that preconditioning of gingival tissue-derived (GMSCs) with tumor necrosis factor-alpha (TNF-α) ideal for treatment periodontitis. TNF-α stimulation not only increased amount secreted from GMSCs, but...
The relation between the concentration of epidermal growth factor (EGF) receptortkinase and effects EGF on cell proliferation has been studied using variant A431 cells antagonist anti-EGF receptor monoclonal antibodies.Clonal variants selected for escape from EGF-mediated inhibition parental all have reduced concentrations receptorkinase; Harvey sarcoma virus-transformed cells, which escaped inhibition, also receptors.Three clonal reacquired 2-to 4fold more than their respective parent...
Patients on high-dose bisphosphonate and immunosuppressive therapy have an increased risk of bisphosphonate-related osteonecrosis the jaw (BRONJ); despite disease severity, its pathophysiology remains unknown, appropriate is not established. Here we developed a mouse model BRONJ-like that recapitulates major clinical radiographic manifestations human disease, including characteristic features open alveolar socket, exposed necrotic bone or sequestra, inflammatory infiltrates, osseous...
Mesenchymal stem cells use the Fas/Fap-1/Cav-1 axis to control vesicle and cytokine secretion accelerate wound healing in mice.
Gingivae represent a unique soft tissue that serves as biological barrier to cover the oral cavity side of maxilla and mandible. Recently, gingivae were identified containing mesenchymal stem cells (GMSCs). However, it is unknown whether GMSCs are derived from cranial neural crest (CNCC) or mesoderm. In this study, we show around 90% CNCC 10% comparison with mesoderm MSCs (M-GMSCs), CNCC-derived (N-GMSCs) an elevated capacity differentiate into chondrocytes induce activated T-cell apoptosis...
Abstract Purpose: Osteonecrosis of the jaw (ONJ) is emerging as one important complications in cancer patients treated with antiresorptive agents. This study explored potential role interleukin (IL)-17–mediated M1/M2 macrophage alterations pathogenesis bisphosphonate-related osteonecrosis (BRONJ). Experimental Design: The expression IL-17 and M1 M2 markers at local mucosal site human BRONJ lesions was examined by immunofluorescence studies. BRONJ-like disease induced C57BL/6 mice multiple...
Despite the promising neuro-regenerative capacities of stem cells, there is currently no licensed cell-based product in repair and regeneration peripheral nerve injuries. Here, we explored potential use human gingiva-derived mesenchymal cells (GMSCs) as only cellular component 3D bio-printed scaffold-free neural constructs that were transplantable to bridge facial defects rats. We showed GMSCs have propensity aggregate into compact 3D-spheroids could produce their own matrix. When cultured...
"Warburg effect", the enhanced glycolysis or aerobic glycolysis, confers cancer cells ability to survive and proliferate even under stressed conditions. In this study, we explored role of epidermal growth factor (EGF) in orchestrating Warburg effect, epithelial-mesenchymal transition (EMT) process, acquisition stem-like cell properties human oral squamous carcinoma (OSCC) cells. Our results showed that EGF induces EMT process OSCC cells, which correlates with properties, including enrichment...
Repair and functional reconstruction of large jawbone defects remain one the challenges in field head neck surgery. The recent progress tissue engineering technologies stem cell biology has significantly promoted development regenerative defects. multiple trophic activities extracellular vesicles (EVs) produced by mesenchymal cells (MSCs) may play a critical role their therapeutic effects. Accumulating evidence shown promise dental pulp (DPSCs) bone regeneration, but less is known about...
Peripheral nerve injuries (PNIs) are common and debilitating, usually resulting in considerable long-term disability remaining an unmet clinical need. Even though the combination of mesenchymal stem cells (MSCs) state-of-the-art tissue engineering technologies has shown promising therapeutic potentials for PNI, there is still not a single licensed cell-based product peripheral repair/regeneration. Emerging evidence indicates that MSC-derived extracellular vesicles (EVs) comparably effective...
Human dental pulp stem cells (DPSCs) can be isolated from inflamed derived carious teeth with symptomatic irreversible pulpitis (I-DPSCs), which possess stemness and multidifferentiation potentials similar to DPSCs healthy pulp. Since macrophages-essential cell players of the pulpal innate immunity-can regulate inflammation repair, authors investigated immunomodulatory effects DPSCs/I-DPSCs on macrophage functions their underlying mechanisms. Similar DPSCs, I-DPSCs were capable...
ABSTRACT Diabetes mellitus is an established risk factor associated with bisphosphonate-related osteonecrosis of the jaw (BRONJ). Sustained activation Nod-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome contributes to persistent inflammation and impaired cutaneous wound healing in diabetic mice human. We have recently demonstrated a compelling linkage between M1 macrophages BRONJ conditions both murine human diseases. The aim this study was determine...
Oral cancer has a high annual incidence rate all over the world, and tongue is most frequently affected anatomic structure. The current standard care ablative surgery of malignant neoplasm, followed by reconstruction with free flap. However, such reconstructive modalities postsurgery radiotherapy or chemotherapy can hardly support functional recovery tongue—particularly, taste bud regeneration—in reconstructed areas, thus seriously affecting patients’ prognosis life quality. Using...
This paper describes results of an experiment with 9 different DARPA Communicator Systems who participated in the June 2000 data collection. All systems supported travel planning and utilized some form mixed-initiative interaction. However they varied several critical dimensions: (1) They targeted back-end databases for information; (2) The used modules ASR, NLU, TTS dialog management. We describe experimental design, approach to collection, metrics collected, comparing systems.
Keloids are characterized as an "overexuberant" healing response in which disequilibrium between production and catabolism of extracellular matrix (ECM) occurs. Previous studies from our laboratory others demonstrate intrinsically higher level plasminogen activator inhibitor-1 (PAI-1) expression keloid tissues cultured fibroblasts compared with normal bordering skin. These findings support the concept that altered balance inhibitor activities system, particular, overexpression PAI-1, may...
Oral premalignancy serves as an ideal model for study of chemopreventive agents. Although 13-cis-retinoic acid showed reversal oral premalignancy, toxicity, and clinical response after cessation therapy obviated its widespread use. A search nontoxic agents with cancer preventive activity led us to evaluate Bowman Birk Inhibitor (BBI) formulated BBI Concentrate (BBIC). We previously reported encouraging results in a phase IIa trial BBIC patients leukoplakia measurable responses favorable...
Abstract Regeneration of peripheral nerve injury remains a major clinical challenge. Recently, mesenchymal stem cells (MSCs) have been considered as potential candidates for regeneration; however, the underlying mechanisms remain elusive. Here, we show that human gingiva-derived MSCs (GMSCs) could be directly induced into multipotent NPCs (iNPCs) under minimally manipulated conditions without introduction exogenous genes. Using crush-injury model rat sciatic nerve, demonstrate GMSCs...