A5042383683- Liver Disease Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Pluripotent Stem Cells Research
- Pancreatic function and diabetes
- Endoplasmic Reticulum Stress and Disease
- Pancreatic and Hepatic Oncology Research
- Circular RNAs in diseases
- Diet, Metabolism, and Disease
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Advanced X-ray Imaging Techniques
- MicroRNA in disease regulation
- Genomics, phytochemicals, and oxidative stress
- Renal and related cancers
- Digital Holography and Microscopy
- Extracellular vesicles in disease
- Optical measurement and interference techniques
- SARS-CoV-2 and COVID-19 Research
- Genomics and Chromatin Dynamics
- COVID-19 Clinical Research Studies
- Mesenchymal stem cell research
- Molecular Biology Techniques and Applications
- Single-cell and spatial transcriptomics
- Microplastics and Plastic Pollution
- Air Quality and Health Impacts
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2017-2025
University College London
2022
The Royal Free Hospital
2022
Istituto Nazionale Genetica Molecolare
2016-2018
University of Trento
2017
University of Siena
1998-2011
Three-dimensional (3D) structured organoids are the most advanced in vitro models for studying human health effects, but their application to evaluate biological effects associated with microplastic exposure was neglected until now. Fibers from synthetic clothes and fabrics a major source of airborne microplastics, release dryer machines is poorly understood. We quantified characterized fibers (MPFs) released exhaust filter household tested on airway (1, 10, 50 µg mL-1) by optical...
Abstract Fatty liver disease (FLD) caused by metabolic dysfunction is the leading cause of and prevalence rising, especially in women. Although during reproductive age women are protected against FLD, for still unknown understudied reasons some develop rapidly progressive at menopause. The patatin-like phospholipase domain-containing 3 ( PNPLA3 ) p.I148M variant accounts largest fraction inherited FLD variability. In present study, we show that there a specific multiplicative interaction...
Breast cancer consists of highly heterogeneous tumors, whose cell origin and driver oncogenes are difficult to be uniquely defined. Here we report that MYC acts as tumor reprogramming factor in mammary epithelial cells by inducing an alternative epigenetic program, which triggers loss identity activation oncogenic pathways. Overexpression induces transcriptional repression lineage-specifying transcription factors, causing decommissioning luminal-specific enhancers. MYC-driven...
Mesenchymal stem cells (MSC) are multipotent able to differentiate into several cell types, hence providing reservoirs for therapeutic applications. The absence of detectable MSC homing at injury sites suggests that paracrine functions could, least in part, be mediated by extracellular vesicles (EVs); EVs newly identified players studied mainly as predictive or diagnostic biomarkers. Together with their clinical interests, have recently come the fore role cell-to-cell communication. In this...
Stem cell identity and plasticity are controlled by master regulatory genes complex circuits also involving non-coding RNAs. Circular RNAs (circRNAs) a class of generated from protein-coding backsplicing, resulting in stable RNA structures devoid free 5' 3' ends. Little is known the mechanisms action circRNAs, let alone stem biology. In this study, for first time, we determined that circRNA controls mesenchymal (MSC) differentiation. High-throughput MSC expression profiling different tissues...
Non-alcoholic fatty liver disease (NAFLD) is the leading cause of disorders and has a strong heritable component. The aim this study was to identify new loci that contribute severe NAFLD by examining rare variants.
Myc family members are critical to maintain embryonic stem cells (ESC) in the undifferentiated state. However, mechanism by which they perform this task has not yet been elucidated. Here we show that directly upregulates transcription of all core components Polycomb repressive complex 2 (PRC2) as well ESC-specific PRC2-associated factors. By expressing protein fused with estrogen receptor (Myc-ER) fibroblasts, observed Myc, binding regulatory elements Suz12, Ezh2, and Eed, induces...
Steatotic liver disease (SLD) prevails as the most common chronic yet lack approved treatments due to incomplete understanding of pathogenesis. Recently, elevated hepatic and circulating interleukin 32 (IL-32) levels were found in individuals with severe SLD. However, mechanistic link between IL-32 intracellular triglyceride metabolism remains be elucidated. We demonstrate vitro that incubation IL-32β protein leads an increase synthesis, while downregulation IL32 by small interfering RNA...
Abstract Stem cell identity depends on the integration of extrinsic and intrinsic signals, which directly influence maintenance their epigenetic state. Although Myc transcription factors play a major role in stem self-renewal pluripotency, with signalling pathways regulators remains poorly defined. We addressed this point by profiling gene expression pattern ESCs whose growth conditional activity. Here we show that potentiates Wnt/β-catenin pathway, cooperates transcriptional regulatory...
Organoids are three-dimensional in vitro-grown cell clusters that recapitulate key features of native organs. In regenerative medicine, organoid technology represents a promising approach for the replacement severely damaged organs, such as pancreas patients with type 1 diabetes. Isolation human organoids (hPOs) chemically defined serum-free culture media would be major milestone this approach.Starting from discarded pancreatic tissues, we developed large-scale process obtaining clinically...
The loss of ovarian functions defining menopause leads to profound metabolic changes and heightens the risk developing dysfunction-associated steatotic liver disease (MASLD). Although estrogens primarily act on female through estrogen receptor alpha (ERα), specific contribution impaired ERα signaling in triggering MASLD after remains unclear.
Gliomas are the most common brain tumors, with diverse biological behaviour. Glioblastoma (GBM), aggressive and worst prognosis, is characterized by an intense aberrant angiogenesis, which distinguishes it from low-grade gliomas (LGGs) benign expansive lesions, as meningiomas (MNGs). With increasing evidence for importance of vascularization in tumor biology, we focused on isolation characterization endothelial cells (ECs) primary GBMs, LGGs MNGs. Gene expression analysis Real-Time PCR,...
Background and Aims: The common genetic variant rs641738 C>T is a risk factor for metabolic dysfunction–associated steatotic liver disease steatohepatitis (MASH), including fibrosis, associated with decreased expression of the phospholipid-remodeling enzyme MBOAT7 (LPIAT1). However, whether restoring in established dampens progression to fibrosis and, importantly, mechanism through which exacerbates MASH remain unclear. Approach Results: We first showed that hepatocyte restoration mice...
Human organoids have been proposed to be powerful tools mimicking the physiopathological processes of organs origin. Recently, human pancreatic (hPOs) gained increasing attention due potential theragnostic and regenerative medicine applications. However, cellular components hPOs not defined precisely. In this work, we finely characterized these structures, focusing first on morphology identity-defining molecular features under long-term culture conditions. Next, focused our cell type...
Mesenchymal stromal cells (MSC) play a critical role in the stem cell niche, specialized microenvironment where reside and interact with surrounding extracellular matrix components. Within MSC offer structural support, modulate inflammatory response, promote angiogenesis release specific signaling molecules that influence behavior, including self-renewal, proliferation differentiation. In epithelial tissues such as intestine, stomach liver, act an important source of cytokines growth...
Fatty liver disease is most frequently related to metabolic dysfunction (MAFLD) and associated comorbidities, heightening the risk of cardiovascular disease, with higher hepatic production IL32, a cytokine linked lipotoxicity endothelial activation. The aim this study was examine relationship between circulating IL32 concentration blood pressure control in individuals at high MAFLD. plasma levels were measured by ELISA 948 enrolled Liver-Bible-2021 cohort. Higher independently systolic...
The therapeutic potential of mesenchymal stem cell (MSC) extracellular vesicles (EV) is currently under investigation in many pathological contexts. Both adult and perinatal MSC are being considered as sources EV. Herein, we address antigen expression cord blood bone marrow released EV to define an identity quality parameter a medicinal product the context clinical applications. research focuses on EV-shuttled neural/glial 2 (NG2), which has previously been detected promising surface marker...
Background & Aims: Non-alcoholic steatohepatitis (NASH)-induced liver fibrosis is emerging as the most common cause of disease.For evaluation therapies, there a pressing need to identify non-invasive, mechanism-based biomarkers.A pro-fibrotic process relevant human NASH involves pathway in which transcriptional regulator called TAZ (WWTR1) hepatocytes induces secretion Indian hedgehog (IHH).We therefore reasoned that circulating IHH may be useful marker assess changes fibrosis.Methods:...
In the last few years, there has been a considerable increase in use of organoids, which is new three-dimensional culture technology applied scientific research. The main reasons for their extensive are plasticity and multiple applications, including regenerative medicine screening drugs. aim this study was to better understand these structures by focusing on choice best housekeeping gene (HKG) perform accurate molecular analysis such heterogeneous system. This feature should not be...
Metabolic dysfunction-associated steatotic liver disease (MASLD) is the leading cause of and its incidence increasing worldwide.1 In patients with MASLD excess accumulation fat linked to metabolic alterations such as insulin resistance, obesity type 2 diabetes.1 encompasses a wide spectrum hepatic ranging from uncomplicated steatosis severe lipotoxicity steatohepatitis (MASH), fibrosis cirrhosis becoming hepatocellular carcinoma then transplantation.1 heterogeneous condition strong heritable...