A5072069420- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Plant Reproductive Biology
- Genetic Mapping and Diversity in Plants and Animals
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Plant Molecular Biology Research
- Plant Genetic and Mutation Studies
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- Chemical and Physical Studies
- CRISPR and Genetic Engineering
- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant biochemistry and biosynthesis
- Biofield Effects and Biophysics
- Biofuel production and bioconversion
- Soybean genetics and cultivation
- Plant Virus Research Studies
- Plant Stress Responses and Tolerance
- Animal Genetics and Reproduction
- Bioenergy crop production and management
- Protist diversity and phylogeny
- Light effects on plants
- RNA Research and Splicing
Temasek Life Sciences Laboratory
2010-2021
National University of Singapore
2010-2021
Chinese Academy of Sciences
2010-2011
Institute of Botany
2010-2011
Bioinformatics Institute
2009
University of the Western Cape
2009
Fujian Academy of Agricultural Sciences
2000
WRKY transcription factors play important roles in the regulation of various biological processes. We have analyzed publicly available rice genome sequence databases and predicted 103 genes encoding factors. Among them, majority (77.7%) were located duplicated regions; 45.6% fragmentally 35% them tandemly duplicated. These results suggested that duplications might be regarded as a major mechanism for expansion this family genome. Subsequently, we their expression profiles under normal...
Abstract Background Sorghum ( bicolor ) is globally produced as a source of food, feed, fiber and fuel. Grain sweet sorghums differ in number important traits, including stem sugar juice accumulation, plant height well grain biomass production. The first whole genome sequence sorghum available, but additional sequences are required to study genome-wide intraspecific variation for dissecting the genetic basis these traits tailor-designed breeding this C 4 crop. Results We resequenced two one...
A two-element Activator/Dissociation (Ac/Ds) gene trap system was successfully established in rice (Oryza sativa ssp. japonica cv. Nipponbare) to generate a collection of stable, unlinked and single-copy Ds transposants. The germinal transposition frequency estimated as an average 51% by analyzing 4413 families. Study pattern siblings revealed that 79% had at least two different insertions, suggesting late during development. Analysis 2057 flanking sequences showed 88% them were unique,...
Plant bZIP transcription factors play crucial roles in multiple biological processes. However, little is known about the sorghum gene family although genome has been completely sequenced. In this study, we have carried out a genome-wide identification and characterization of sorghum. Our data show that encodes at least 92 factors. These genes expanded mainly by segmental duplication. Such an expansion mechanism also observed rice, arabidopsis many other plant organisms, suggesting common...
Lectins are a class of carbohydrate-binding proteins. They play roles in various biological processes. However, little is known about their evolutionary history and functions plant stress regulation. The availability full genome sequences from species makes it possible to perform whole-genome exploration for further understanding functions.Higher genomes encode large numbers lectin Based on domain structures phylogenetic analyses, new classification system has been proposed. In this system,...
Abstract Terpenes are organic compounds and play important roles in plant growth development as well mediating interactions of plants with the environment. Terpene synthases (TPSs) key enzymes responsible for biosynthesis terpenes. Although some species were employed genome-wide identification characterization TPS family, limited information is available regarding evolution, expansion, retention mechanisms occurring this gene family. We performed a family members 50 sequenced genomes....
Glutathione S-transferases (GSTs) exist in various eukaryotes and function detoxification of xenobiotics response to abiotic biotic stresses. We have carried out a genome-wide survey this gene family 10 plant genomes. Our data show that tandem duplication has been regarded as the major expansion mechanism both monocot dicot plants may practiced different evolutionary history. Non-synonymous substitutions per site (Ka) synonymous (Ks) analyses showed N- C-terminal functional domains GSTs...
Abstract Sucrose, as the main product of photosynthesis, plays crucial roles in plant development. Although studies on general metabolism pathway were well documented, less information is available genome-wide identification these genes, their expansion and evolutionary history biological functions. We focused four sucrose related gene families including synthase, phosphate phosphatase UDP-glucose pyrophosphorylase. These exhibited different host genomes experienced differentiated rates...
Abstract Rice grains are rich in starch but low protein with very level of both lysine and threonine. Thus, it is important to further improve quality quantity, especially increase threonine content rice grains. We artificially synthesized two new genes by fusing endogenous (K)/threonine (T) motif (TKTKK) coding sequences. They were designated as TKTKK1 TKTKK2 their encoded proteins consist 73.1% 83.5% lysine/threonine, respectively. These under the control 35S promoter independently...
Valine-glutamine (VQ) motif containing proteins play important roles in abiotic and biotic stress responses plants. However, little is known about the origin evolution as well comprehensive expression regulation of VQ gene family. In this study, we systematically surveyed family 50 plant genomes from algae, moss, gymnosperm angiosperm explored their presence other species animals, bacteria, fungi viruses. No VQs were detected all tested algae encode varying numbers VQs. Interestingly, some...
Both small GTPase and its activating protein (GAP) superfamilies exist in various eukaryotes. The GTPases regulate a wide variety of cellular processes by cycling between active GTP- inactive GAP-bound conformations. GAPs promote inactivation stimulating the GTP hydrolysis. In this study, we identified 111 85 rice, 65 Arabidopsis, 90 Drosophila melanogaster, 35 Saccharomyces cerevisiaeby genome-wide analysis. We then analyzed compared total 498 422 from these four eukaryotic human genomes....
Background Plant cytochromes P450 are involved in a wide range of biosynthetic reactions and play various roles plant development. However, little is known about the biological functions subfamily CYP96 plants. Methodology/Principal Findings Here, we report novel semi-dwarf rice mutant, which single copy transposon dissociator (Ds) was inserted into gene OsCYP96B4 (Oryza sativa Cytochrome 96B4). The mutant exhibits defects cell elongation pollen germination, can be complemented by wild type...
Tandem and segmental duplications significantly contribute to gene family expansion genome evolution. Genome-wide identification of tandem genes has been analyzed before in several plant genomes. However, comparative studies functional bias, expression divergence their roles species domestication are still lacking. We have carried out a genome-wide analysis the rice genome. A total 3,646 3,633 pairs genes, respectively, were identified They made up around 30% annotated (excluding...
Long terminal repeat (LTR) retrotransposons are the major class I mobile elements in plants. They play crucial roles gene expansion, diversification and evolution. However, their captured genes yet to be genome-widely identified characterized most of plants although many genomes have been completely sequenced. In this study, we 7,043 23,915 full-length LTR rice sorghum genomes, respectively. High percentages were distributed near centromeric region each chromosomes. contrast, not enriched...
Biological scientists have long sought after understanding how genes and their structural/functional changes contribute to morphological diversity. Though both grain (BT×623) sweet (Keller) sorghum lines originated from the same species Sorghum bicolor L., they exhibit obvious phenotypic variations. However, genome re-sequencing data revealed that exhibited limited functional diversity in encoding a genome-wide level. The result raises question variations between occurred relatively short...
Small-sized droplets/aerosol transmission is one of the factors responsible for spread COVID-19, in addition to large droplets and surface contamination (fomites). While can be relatively easier deal with (i.e., using mask proper hygiene measures), aerosol presents a different challenge due their ability remain airborne long time. This calls mitigation solutions that rapidly eliminate aerosol. Pre-COVID-19, air ionizers have been touted as effective tools small particulates. In this work, we...
Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. However, J. susceptible to the geminivirus Indian cassava mosaic virus (ICMV), frequent viral disease outbreaks severely limit productivity. Therefore development of carry on durable resistance remains crucial poses major biotechnological challenge.
Particulate matter (PM, especially PM2.5 with diameter 2.5 microns) has been regarded as the major air pollutant. Negative ions (NAIs) could electrically charge PM and remove it much more efficiently. In this study, a bio-generator of NAIs developed, which helps plants to generate at around 100 × 106 ions/cm3 under pulsed electrical field (PEF) treatment. By using bio-generator, concentration in growth chamber be reduced from 500 near 0 µg/m3 within 5 minutes. It also used continuously...