A5022938364- Caveolin-1 and cellular processes
- RNA Research and Splicing
- Central Venous Catheters and Hemodialysis
- Neuroscience of respiration and sleep
- Neonatal Respiratory Health Research
- Neonatal and fetal brain pathology
- Inflammatory Bowel Disease
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- RNA and protein synthesis mechanisms
- Venous Thromboembolism Diagnosis and Management
- Sports Performance and Training
- Cardiovascular and exercise physiology
- Anesthesia and Neurotoxicity Research
- Complement system in diseases
- Malaria Research and Control
- Obstructive Sleep Apnea Research
- Nanoparticles: synthesis and applications
- Colorectal Cancer Screening and Detection
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Nausea and vomiting management
- HER2/EGFR in Cancer Research
- Diet and metabolism studies
- Gastric Cancer Management and Outcomes
QIMR Berghofer Medical Research Institute
2018-2025
Sapporo Higashi Tokushukai Hospital
2025
IRYO Sosei University
2021-2025
The University of Queensland
2009-2024
Royal Brisbane and Women's Hospital
2021-2024
Shinshu University
2017-2023
University Hospitals Bristol and Weston NHS Foundation Trust
2021
University of Bristol
2017-2021
Mater Research
2019-2021
St Michael's Hospital
2021
In mammalian cells three closely related cavin proteins cooperate with the scaffolding protein caveolin to form membrane invaginations known as caveolae. Here we have developed a novel single-molecule fluorescence approach directly observe interactions and stoichiometries in complexes from cell extracts vitro synthesized components. We show that up 50 cavins associate on caveola. However, rather than forming single coat complex containing family members, analysis reveals an exquisite...
Abstract Titanium plates are widely used in clinical settings because of their high bone affinity. However, owing to elastic modulus, these not suitable for repair since proximity the surface prolonged periods can cause stress shielding, leading embrittlement. In contrast, titanium fiber prepared by molding fibers into simultaneously applying compression and shear at normal room temperature have an modulus similar that cortex, shielding will occur even when plate lies flush against bone's...
The generation of caveolae involves insertion the cholesterol-binding integral membrane protein caveolin-1 (Cav1) into membrane, however, precise molecular mechanisms are as yet unknown. We have speculated that caveolin scaffolding domain (CSD), a conserved amphipathic region implicated in interactions with signaling proteins, is crucial for caveola formation. now define core membrane-juxtaposed Cav1 and show oligomerization CSD protected by tight association both mature mammalian model...
Abstract Caveolae are specialized domains of the plasma membrane. Formation these invaginations is dependent on expression Caveolin-1 or -3 and proteins cavin family. In response to stress, caveolae disassemble cavins released from caveolae, allowing potentially interact with intracellular targets. Here, we describe (non-plasma membrane) interactome using biotin affinity proteomics mass spectrometry. We validate 47 potential cavin-interactor a cell-free system protein-protein binding assays....
Abstract Non-alcoholic fatty liver disease (NAFLD) affects 25% of the adult population globally. Since fibrosis is most important predictor liver-related complications in patients with NAFLD, identification advanced among at-risk individuals an issue clinical practice. Transient elastography best evaluated non-invasive method used referral centres to assess fibrosis, however serum-based tests, such as Enhanced Liver Fibrosis (ELF) score, have a practical advantage first-line tests due their...
Caveolin-1 (CAV1) is an important regulator of adipose tissue homeostasis. In the present study we examined impact CAV1 deficiency on properties mouse both in vivo and explant cultures during conditions metabolic stress. CAV1−/− mice fasting caused loss mass despite a lack hormone-sensitive lipase (HSL) phosphorylation. addition, resulted increased macrophage infiltration, enhanced deposition collagen, reduction level lipid droplet protein perilipin A (PLIN1a). Explant also showed PLIN1a...
Pluripotent stem cell-derived cardiomyocytes show great promise in regenerating the heart after myocardial infarction; however, several uncertainties exist that must be addressed before clinical trials. One practical issue is graft survival following transplantation. Although a pro-survival cocktail with Matrigel has been shown to enhance survival, use of may not clinically feasible. The purpose this study was test whether hyaluronan-based hydrogel, HyStem, could substitute for Matrigel....
T-follicular helper (Tfh) cells are key drivers of antibodies that protect from malaria. However, little is known regarding the host and parasite factors influence Tfh functional antibody development. Here, we use samples a large cross-sectional study children residing in an area high malaria transmission Uganda to characterize multiple parasites stages. We identify dramatic re-distribution cell compartment with age independent exposure, Th2-Tfh predominating early childhood, while Th1-Tfh...
CAV1 (caveolin 1) expression and secretion is associated with prostate cancer (PCa) disease progression, but the mechanisms underpinning release remain poorly understood. Numerous studies have shown can be secreted within exosome-like vesicles, antibody-mediated neutralization mitigate PCa progression; this suggestive of an inverted (non-exosomal) topology. Here we show that from specific types in vesicle-associated form consistent features bioactive secretion. Characterization isolated...
Peripheral nerve blocks can provide surgical anaesthesia as well excellent postoperative analgesia. When questioned postoperatively, however, some patients report low levels of satisfaction with their block experience. At our hospital, undergoing regional have patient characteristics, characteristics and feedback routinely recorded in a registry. We analysed data from 979 consecutive peripheral for orthopaedic surgery to identify factors associated satisfaction. The primary outcome was...
Abstract Background Central venous access devices (CVAD) are commonly employed in the management of cancer patients. While having several benefits they associated with significant risks. Aim To review incidence and risk factors for catheter‐related thrombosis (CRT) patients a CVAD. Methods We performed prospective observational cohort study adult requiring CVAD between 1 January 2004 29 June 2016. The rate of, development symptomatic CRT were evaluated. Results A total 4920 central lines was...
There is a lack of certainty in dietary prescription for individuals with inflammatory bowel disease (IBD) due to heterogeneity studies date. The aim this study was investigate the efficacy on activity modified anti-inflammatory pattern purposely designed reduce intake food additives (IBD-MAID), compared standard care, adults IBD.
[Purpose] To evaluate the validity of measurement sites for assessing blood lactic acid levels following unilateral upper-limb exercises. [Participants and Methods] Blood were measured at fingertips both hands in 40 healthy young men (mean age 20.9 ± 2.5 years). Measurements taken before, immediately after, 90-s intervals exercises involving palmar flexion dorsiflexion non-dominant hand. Exercise load was determined through maximum voluntary contraction testing. [Results] No significant...
Abstract One of the main goals human genome project was to identify all protein-coding genes. There are ∼ 20,500 genes annotated in reference databases. However, last few years, proteogenomics studies have predicted thousands novel regions including low molecular weight proteins encoded by small open reading frames (ORFs) untranslated messenger RNAs and non-coding RNAs. Most these predictions based on bioinformatics analysis ribosome footprints. The validity some ORF (sORF) (SEPs) has been...
Autoimmune glial fibrillary acidic protein (GFAP) astrocytopathy is a new spectrum of autoimmune inflammatory nervous system disorders associated with infection, neoplasm, or drug use. We present the first pediatric case GFAP caused by an influenza A infection. The patient manifested meningoencephalitis, central respiratory failure, quadriplegia, elimination disorders, and syndrome inappropriate antidiuretic hormone secretion. Methylprednisolone pulse therapy led to good prognosis. In cases...
The scarcity of effective biomarkers and therapeutic strategies for predicting disease onset progression in neurodegenerative diseases such as Alzheimer's (AD) is a major challenge. Conventional drug discovery approaches have been unsuccessful providing efficient interventions due to their 'one-size-fits-all' nature. As an alternative, personalised development holds promise pre-select responders identifying suitable indicators efficacy. In this exploratory study, we established pipeline with...
Management of malignant insulinoma (MI) presents dual management challenges hypoglycaemia and tumour control. This study aims to analyse long-term outcomes PRRT for treatment MI. We retrospectively reviewed consecutive patients with MI treated [177Lu]Lu-DOTATATE (LuTATE) at two Australian NET centres between 2004-2022. Follow-up hypoglycaemia, molecular imaging, radiologic biochemical responses, treatment-related side-effects, progression free- overall-survival were assessed. Of 15 (7...
Purpose: Although both anti-PD-1 antibody and treatments using anti-PD-L1 are currently in clinical use, their therapeutic effects vary according to cancer type.One of the factors accounting for this variability is expression level immune checkpoint molecule that differs between types; thus, it important clarify relationship outcomes molecules all types human cancer.The purpose study evaluate outcome osteosarcoma relation PD-L1, PRF, GZMB, IFNγ expression.Methods: Using 19 specimens...
Abstract Background Psychosocial assessment should be part of clinic visits for people with diabetes mellitus (DM). Aims To assess the usage and acceptance a psychosocial tool (DPAT) to profile clinical characteristics young diabetes. Methods Over 12‐month period, adults (18–25 years) attending were offered DPAT. The embeds validated screening tools including Problem Areas in Diabetes 20 (PAID‐20) questionnaire, Patient Health Questionnaire‐4 (PHQ‐4) World Organization Well‐Being Index‐5...
Abstract Background Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat heart diseases; however, the optimal maturity of hiPSC-CMs for effective regenerative medicine remains unclear. We aimed investigate benefits long-term cultured mature in injured rat hearts. Methods Cardiomyocytes were differentiated from hiPSCs via monolayer culturing, and cells harvested on day 28 or 56 (D28-CMs D56-CMs, respectively) after differentiation. transplanted D28-CMs...
Summary Background An episode of acute ulcerative colitis (UC) represents an important watershed moment in a patient's disease course. Aims To derive personalised algorithm for identifying patients at high risk corticosteroid non‐response from variables available hospital presentation using large prospectively collected UC patient database and machine learning‐based techniques. Methods We analysed data 682 consecutive presentations UC. used Akaike information criterion‐based elastic net...