A5077990905- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Lung Cancer Treatments and Mutations
- Traditional Chinese Medicine Analysis
- Horticultural and Viticultural Research
- Biochemical and biochemical processes
- Enzyme Production and Characterization
- Food Quality and Safety Studies
- Nephrotoxicity and Medicinal Plants
- Protist diversity and phylogeny
- Phytochemicals and Antioxidant Activities
- Traditional Chinese Medicine Studies
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Research Studies
- Drug-Induced Hepatotoxicity and Protection
- RNA modifications and cancer
- Tuberculosis Research and Epidemiology
- Medicinal Plant Pharmacodynamics Research
- Radiomics and Machine Learning in Medical Imaging
- Parasitic infections in humans and animals
- Congenital Anomalies and Fetal Surgery
Tianjin University of Science and Technology
2015-2024
Ningxia Center for Diseases Prevention and Control
2024
Ningxia Medical University
2015-2024
Chongqing Medical University
2018-2024
Xian Yang Central Hospital
2012-2024
Inner Mongolia People's Hospital
2017-2023
Beijing Chao-Yang Hospital
2023
Capital Medical University
2023
Harbin Medical University
2022-2023
Third Affiliated Hospital of Harbin Medical University
2022-2023
Limonene is an important biologically active natural product widely used in the food, cosmetic, nutraceutical and pharmaceutical industries. However, low abundance of limonene plants renders their isolation from plant sources non-economically viable. Therefore, engineering microbes into microbial factories for producing fast becoming attractive alternative approach that can overcome aforementioned bottleneck to meet needs industries make production more sustainable environmentally...
Summary The natural plant product bisabolene serves as a precursor for the production of wide range industrially relevant chemicals. However, low abundance in plants renders its isolation from sources non‐economically viable. Therefore, creation microbial cell factories supported by synthetic biology and metabolic engineering strategies presents more competitive environmentally sustainable method industrial bisabolene. In this proof‐of‐principle study, first time, we engineered oleaginous...
Limonene is an important plant natural product widely used in food and cosmetics production as well the pharmaceutical chemical industries. However, low efficiency of extraction high energy consumption synthesis limit sustainability industrial limonene production. Recently, advancement metabolic engineering synthetic biology has facilitated microbes into microbial cell factories for producing limonene. deleterious effects on cellular activity by toxicity major obstacle achieving high-titer...
2,3-Butanediol (2,3-BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, Klebsiella pneumoniae the most powerful 2,3-BD producer utilize wide range of substrates. However, many by-products are K. pneumoniae, such as ethanol, lactate, and acetate, negatively regulate yield increase costs downstream separation purification.In this study, we constructed mutants with lactate dehydrogenase (LDH), acetaldehyde (ADH),...
Itaconic acid (IA) is a high-value organic with plethora of industrial applications. In this study, we seek to develop microbial cell factory that could utilize waste cooking oil (WCO) as raw material for circular and cost-effective production the abovementioned biochemical. Specifically, expressed cis-aconitic decarboxylase (CAD) gene from Aspergillus terreus in either cytosol or peroxisome Yarrowia lipolytica assayed IA on WCO. To further improve yield, 10 genes involved pathway...
Establishing efficient synthetic pathways for microbial production of biochemicals is often hampered by competing and insufficient precursor supply.
Squalene is a highly sought-after triterpene compound in growing demand, and its production offers promising avenue for circular economy practices. In this study, we applied metabolic engineering principles to enhance squalene the nonconventional yeast
Abstract Background In biological cells, promoters drive gene expression by specific binding of RNA polymerase. They determine the starting position, timing and level expression. Therefore, rational fine-tuning to regulate levels target genes for optimizing biosynthetic pathways in metabolic engineering has recently become an active area research. Results this study, we systematically detected characterized common promoter elements unconventional yeast Yarrowia lipolytica , constructed...
Bisabolene is a bioactive sesquiterpene with wide range of applications in food, cosmetics, medicine, and aviation fuels. Microbial production offers green, efficient, sustainable alternative. In this study, we focused on improving the titers α-bisabolene Yarrowia lipolytica by applying two strategies, (i) optimizing metabolic flux biosynthetic pathway (ii) sequestering lipid droplet, thus alleviating its inherent toxicity to host cells. We showed that overexpression DGA1 OLE1 increase...
Silicon (Si) has an important function in reducing the damage of environmental stress on plants. Acid rain is a serious abiotic factor, and Si can alleviate induced by acid Based these assumptions, we investigated effects silicon growth, root phenotype, mineral element contents, hydrogen peroxide (H2O2) antioxidative enzymes rice (Oryza sativa L.) seedling roots under simulated (SAR) stress. The results showed that combined or single and/or SAR depend concentration pH SAR. low moderate (1.0...
The effect of shading during seed development on subsequent germination remains largely unknown. In this study, two soybean (Glycine max) production systems, monocropping (MC) and maize-soybean intercropping (IC), were employed to examine the effects mother plant germination. Compared MC seeds, which received light, developing IC seeds exposed shade resulting from taller neighboring maize plants. germinated faster than although there was no significant difference in thickness coat....
Abstract It’s a challenge for detecting the therapeutic targets of polypharmacological drug from variations in responsed networks differentiated populations with complex diseases, as stable coronary heart disease. Here, an adaptive, 31-center, randomized, double-blind trial involving 920 patients moderate symptomatic angina treated by 14-day Danhong injection(DHI), kind high quality control, or placebo (0.9% saline), 76-day following-up, we firstly confirmed that DHI could increase...
Abstract Background Immune checkpoint inhibitors (ICIs) are the standard treatment for advanced lung cancer, but immune‐related adverse events (irAEs) remain poorly understood, especially in a real‐world setting. Methods A multicenter observational study was conducted. Medical records of cancer patients treated with ICIs at 26 hospitals from January 1, 2015, to February 28, 2021, were retrieved. Types included antiprogrammed cell death 1 or ligand (PD‐L1) monotherapy, anticytotoxic...
Metabolic engineering of yeasts for terpenoid production has mostly focused on the cytoplasm, whereas harnessing their organelles as subcellular factories been overlooked. Herein, farnesyl diphosphate synthetic pathway and α-bisabolene synthase were compartmentalized into oleaginous yeast Yarrowia lipolytica's mitochondria to enable high-level production. Through comprehensive metabolic approaches, we exploited potential capability a factory achieve 257.4 mg/L from glucose. By combining...