A5081820555- Gut microbiota and health
- Diet and metabolism studies
- Spaceflight effects on biology
- Stress Responses and Cortisol
- Yersinia bacterium, plague, ectoparasites research
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Tryptophan and brain disorders
- Cancer-related molecular mechanisms research
- High Altitude and Hypoxia
- Anesthesia and Neurotoxicity Research
- RNA Research and Splicing
- Biochemical effects in animals
- Pesticide Exposure and Toxicity
- Posttraumatic Stress Disorder Research
- Molecular Biology Techniques and Applications
- Complementary and Alternative Medicine Studies
- Traumatic Brain Injury Research
- Antimicrobial Peptides and Activities
- Cell Adhesion Molecules Research
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Genetics and Physical Performance
- Dietary Effects on Health
- Adipose Tissue and Metabolism
Walter Reed Army Institute of Research
2020-2025
Oak Ridge Institute for Science and Education
2018-2022
Oak Ridge Associated Universities
2018-2022
Center for Environmental Health
2012-2019
United States Army
2013-2018
The Geneva Foundation
2015-2018
United States Army Medical Research and Development Command
2012
United States Army Corps of Engineers
2012
The magnitude, temporal dynamics, and physiological effects of intestinal microbiome responses to stress are poorly characterized. This study used a systems biology approach multiple-stressor military training environment determine the on microbiota composition metabolic activity, as well permeability (IP). Soldiers (n = 73) were provided three rations per day with or without protein- carbohydrate-based supplements during 4-day cross-country ski-march (STRESS). IP was measured before STRESS....
A social-stress mouse model was used to simulate features of post-traumatic stress disorder (PTSD). The involved exposure an intruder (male C57BL/6) a resident aggressor SJL) for 5 or 10 consecutive days. Transcriptome changes in brain regions (hippocampus, amygdala, medial prefrontal cortex and hemibrain), blood spleen as well epigenome the hemibrain were assayed after 1- 10-day intervals following 5-day trauma 42-day trauma. Analyses differentially expressed genes (common among brain,...
The bidirectional role of gut-brain axis that integrates the gut and central nervous system activities has recently been investigated. We studied "cage-within-cage resident-intruder" all-male model, where subject male mice (C57BL/6J) are exposed to aggressor (SJL albino), microbiota-derived metabolites were identified in plasma after 10 days exposure. assessed 16S ribosomal RNA gene from fecal samples collected daily these during 10-day study. Alpha diversity using Chao indices indicated no...
Gene expression profiling using blood samples is a valuable tool for biomarker discovery in clinical studies. Different whole RNA collection and processing methods are highly variable might confound comparisons of results across The main aim the current study to compare how storage, extraction methodologies, components themselves may influence gene profiling. Whole peripheral mononuclear cell (PBMC) were collected triplicate from five healthy donors. was RNAgard® PAXgene® Blood Tubes, as...
miRNAs act as important regulators of gene expression by promoting mRNA degradation or attenuating protein translation. Since are stably expressed in bodily fluids, there is growing interest profiling these miRNAs, it minimally invasive and cost-effective a diagnostic matrix. A technical hurdle studying miRNA dynamics the ability to reliably extract small sample volumes low RNA abundance create challenges for extraction downstream applications. The purpose this study was develop pipeline...
Abstract Post-traumatic stress disorder (PTSD) is a heterogeneous condition evidenced by the absence of objective physiological measurements applicable to all who meet criteria for as well divergent responses treatments. This study capitalized on biological diversity observed within PTSD group following epigenome-wide analysis well-characterized Discovery cohort ( N = 166) consisting 83 male combat exposed veterans with PTSD, and without in order identify patterns that might distinguish...
Metabolomics, proteomics and DNA methylome assays, when done in tandem from the same blood sample analyzed together, offer an opportunity to evaluate molecular basis of post-traumatic stress disorder (PTSD) course pathogenesis. We performed separate metabolomics, proteomics, assays on samples two well-characterized cohorts 159 active duty male participants with relatively recent onset PTSD (<1.5 years) 300 veterans chronic (>7 years). Analyses multi-omics datasets these independent were used...
Abstract Sleep restriction alters gut microbiota composition and intestinal barrier function in rodents, but whether similar effects occur humans is unclear. This study aimed to determine the of severe, short-term sleep on permeability healthy adults. Fecal composition, measured by 16S rRNA sequencing, were 19 men (mean ± SD; BMI 24.4 2.3 kg/m 2 , 20 years) undergoing three consecutive nights adequate (AS; 7–9 h sleep/night) restricted (SR; random order with controlled diet physical...
There is an escalating need to comprehend the long-term impacts of nuclear radiation exposure since permeation ionizing has been frequent in our current societal framework. A system evaluation microbes that reside inside a host's colon could meet this knowledge gap play major roles response stress. Indeed, past study suggested these might break their symbiotic association with moribund hosts form pro-survival condition exclusive themselves. In study, we undertook metagenomics and...
Post-traumatic stress disorder (PTSD) is a complex, debilitating condition prevalent among military personnel exposed to traumatic events, necessitating biomarkers for early detection and intervention. Using data from the Millennium Cohort Study, largest longest-running health study initiated in 2001, our objective was identify specific microRNA (miRNA) expression patterns associated with distinct PTSD symptom trajectories service members veterans assess their potential predicting resilience...
Prolonged residence of mice in spaceflight is a scientifically robust and ethically ratified model muscle atrophy caused by continued unloading. Under the Rodent Research Program National Aeronautics Space Administration (NASA), we assayed large-scale mRNA metabolomic perturbations quadriceps C57BL/6j male that lived (FLT) or on ground (control CTR) for approximately 4 weeks. The wet weights were significantly reduced FLT mice. Next-generation sequencing untargeted mass spectroscopic assays...
Spaceflight presents a spectrum of stresses very different from those associated with terrestrial conditions. Our previous study (BMC Genom. 15: 659, 2014) integrated the expressions mRNAs, microRNAs, and proteins results indicated that microgravity induces an immunosuppressive state can facilitate opportunistic pathogenic attack. However, existing data are not sufficient for elucidating molecular drivers given immunosuppressed state. To meet this knowledge gap, we focused on metabolite...
The association between the shift in fecal resident microbiome and social conflicts with long-term consequences on psychological plasticity, such as development of post-traumatic stress disorder (PTSD), is yet to be comprehended. We developed an aggressor-exposed (Agg-E) (SS) mouse model mimic warzone-like conflicts, where random life-threatening interactions took place naïve intruder mice aggressive mice. Gradually these Agg-E distinct characteristics simulating PTSD-like aspects, whereas...
Pneumonic plague is caused by the aerosolized form of Yersinia pestis and a highly virulent infection with complex clinical consequences, without treatment, fatality rate approaches 100%. The exact mechanisms disease progression are unclear, limited work done using metabolite profiling to study progression.The aim this pilot was profile plasma metabolomics in an animal model Y. infection.In study, African Green monkeys were challenged virulent, strain CO92, untargeted performed liquid gas...
Microorganisms that colonize in or on a host play significant roles regulating the host's immunological fitness and bioenergy production, thus controlling stress responses. Radiation elicits pro-inflammatory bioenergy-expensive state, which could influence gut microbial compositions and, therefore, host-microbe bidirectional relationship. To test this hypothesis, young adult mice were exposed to total body irradiation (TBI) at doses of 9.5 Gy 11 Gy, respectively. The irradiated euthanized...
The experimental pathophysiology of organophosphorus (OP) chemical exposure has been extensively reported. Here, we describe an altered fecal bacterial biota and urine metabolome following intoxication with soman, a lipophilic G class warfare nerve agent. Nonanesthetized Sprague-Dawley male rats were subcutaneously administered soman at 0.8 (subseizurogenic) or 1.0 (seizurogenic) the 50% lethal dose (LD50) evaluated for signs toxicity. Animals stratified based on seizing activity to evaluate...
Introduction Sequelae from traumatic brain injuries (TBIs) and post-traumatic stress disorder (PTSD) are major career-limiting factors for combat soldiers. Overlap between TBI PTSD symptoms alongside other common comorbidities complicate the diagnosis treatment. Systems-level high-throughput approaches key in understanding underlying biomolecular mechanisms differentiating these conditions. Methods The present study identifies dietary proposes behind psychological TBI, using established...
Abstract Emerging evidence suggests a significant role of gut microbiome in bone health. Aging is well recognized as crucial factor influencing the microbiome. In this study, we investigated whether age-dependent microbial change contributes to age-related loss CB6F1 mice. The phenotype 24-month-old germ-free (GF) mice was indistinguishable compared their littermates colonized by fecal transplant at 1-month-old. Moreover, from 3 comparable between GF and specific pathogen-free (SPF) Thus,...
Rehabilitation from musculoskeletal injuries (MSKI) complicate healing dynamics typically by sustained disuse of bone and muscles. Microgravity naturally allows limb thus an effective model to understand MSKI. The current study examined epigenetic changes in a segmental defect (SBD) mouse prolonged unloading condition after spaceflight (FLT). We further connected potential miRNA–mRNA regulatory pathways impacting healing. Here, SBD surgery was performed on nine-week-old male mice that were...
Yersinia pestis , the etiological agent of plague, is a potential biothreat agent. Upon inhalation, Gram‐negative bacterium causes pneumonic rapidly progressing, severe lung infection that usually fatal. Only limited work using metabolite profiles to study progression disease has been done, and exact mechanisms facilitate are not well‐characterized. In this study, African Green monkeys were exposed aerosolized highly virulent Y. CO92 metabolomic profiling plasma samples was conducted....