A5102976619- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Gastrointestinal motility and disorders
- Mitochondrial Function and Pathology
- Eosinophilic Disorders and Syndromes
- Clinical Nutrition and Gastroenterology
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Congenital gastrointestinal and neural anomalies
- X-ray Diffraction in Crystallography
- thermodynamics and calorimetric analyses
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Diabetes Management and Research
- Psoriasis: Treatment and Pathogenesis
- Microfluidic and Capillary Electrophoresis Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Retinal and Macular Surgery
- Diet and metabolism studies
- Cardiac electrophysiology and arrhythmias
- Gut microbiota and health
- Pickering emulsions and particle stabilization
- Ion channel regulation and function
- Cellular Mechanics and Interactions
- Nanocomposite Films for Food Packaging
KU Leuven
2020-2024
Children's Oncology Group
2022
University of Vermont
2022
Hasselt University
2022
VIB-KU Leuven Center for Brain & Disease Research
2022
Montavid Thermodynamic Research Group
2022
Ghent University
2016
Correct spatiotemporal distribution of organelles and vesicles is crucial for healthy cell functioning regulated by intracellular transport mechanisms. Controlled bulky mitochondria especially important in polarized cells such as neurons that rely on these to locally produce energy buffer calcium. Mitochondrial requires depends microtubules fill much the available axonal space. How mitochondrial affected their position within microtubule bundles not known. Here, we found anterograde...
Abstract Background Gastrointestinal (GI) function is critically dependent on the control of enteric nervous system (ENS), which situated within gut wall and organized into two ganglionated nerve plexuses: submucosal myenteric plexus. The ENS optimally positioned together with intestinal epithelium, well‐equipped to monitor luminal contents such as microbial metabolites coordinate appropriate responses accordingly. Despite heightened interest in microbiota its influence physiology...
Abstract Three-dimensional tissue spheroids are a key building block in biofabrication, yet the link between their material properties and active mechanics of individual cells is not fully understood. We study small human periosteum-derived as they effect spheroid fusion, an elementary operation for constructing large structures. use two-photon confocal microscopy to measure cell-cell tension cell motility throughout fusion. Cytoskeletal inhibition through Y-27632 (ROCKi) results more...
Eosinophils were previously reported to play a role in intestinal inflammation and fibrosis. Whether this is as bystander or an active participant still up for debate. Moreover, data describing causal relationship between eosinophils fibrosis are scarce. We here aimed elucidate the of pathogenesis Therefore, we stimulated fibroblasts with (active) Eosinophil Cationic Protein (ECP), assessed fibroblast activation via flow cytometry immunocytochemistry. observed decreased when co-cultured...
Abstract Despite the importance of cell characterization and identification for diagnostic therapeutic applications, developing fast label‐free methods without (bio)‐chemical markers or surface‐engineered receptors remains challenging. Here, we exploit natural cellular response to mild thermal stimuli propose a label‐ receptor‐free method facile characterization. Cell suspensions in dedicated sensor are exposed temperature gradient, which stimulates synchronized spontaneous cell‐detachment...
Summary Correct development and maturation of the enteric nervous system (ENS) is critical for survival. Early in life, ENS requires significant refinement order to adapt evolving needs tissue, changing from milk solid food at time weaning. Here, we demonstrate that resident macrophages muscularis externa, MMϕ, refine early life by pruning synapses phagocytosing abundant neurons. After weaning, MMϕ continue closely interact with ENS, acquire a microglia-like phenotype are crucial survival Of...
Abstract Objectives To investigate whether physical activity interferes with joint homeostasis in the presence of distant inflammation originating at barrier tissues such as skin or gut. Methods Eight-week-old male C57/Bl6 mice were treated imiquimod cream on a shaved area back dextran sodium sulphate dissolved drinking water to induce psoriasis-like inflammatory bowel disease-like gut inflammation. Afterwards, one group was subjected 4-week forced running routine (n = 10 per group)....
Abstract Background In several disease areas, such as in the liver and lungs, a pro-fibrotic role for eosinophils has been suggested. However, information about of development intestinal fibrosis is scarce. We therefore aimed to assess functional mechanistic fibroblast activation. Methods Chronic DSS colitis (3 cycles 1 week administration followed by 2 weeks recovery (1.75% - 2.25% 2.25%)) was introduced 6-8-week-old C57BL/6 RAG-/- mice induce (Figure 1A). Mice were injected...
Background: Eosinophils were previously reported to play a role in intestinal inflammation and fibrosis. Whether this is as bystander or an active participant still up for debate. Moreover, data describing causal relationship between eosinophils fibrosis are scarce. Therefore, we aimed elucidate the of pathogenesis fibrosis.Methods: In that context, stimulated fibroblasts with Eosinophil Cationic Protein assessed fibroblast activation via flow cytometry immunocytochemistry. Furthermore,...
Summary Intravitreal injection is an interesting delivery route for the administration of nanoparticles treatment a variety retinopathies. To reach retina, need to diffuse through vitreous and travel across vitreoretinal interface. We have recently optimized ex vivo assay that allows measure mobility fluorescent in intact humour on single‐particle level. With this model, we optimize surface coating strategies assure good vitreal mobility, while maintaining normal transfection efficiency....
Abstract Correct spatiotemporal distribution of organelles and vesicles is crucial for healthy cell functioning regulated by intracellular transport mechanisms. Controlled bulky mitochondria especially important in polarized cells such as neurons that rely on these to locally produce energy buffer calcium. Mitochondrial requires depends microtubules which fill much the available axonal space. How mitochondrial affected their position within microtubule bundles not known. Here, we found...