A5043701805- Receptor Mechanisms and Signaling
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Protein Kinase Regulation and GTPase Signaling
- Fungal Biology and Applications
- Photoreceptor and optogenetics research
- Retinal Development and Disorders
- Natural product bioactivities and synthesis
- Kruppel-like factors research
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Mast cells and histamine
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Signaling Pathways in Disease
- Microbial Natural Products and Biosynthesis
- Bone Metabolism and Diseases
- Hippo pathway signaling and YAP/TAZ
- Cytokine Signaling Pathways and Interactions
- Histone Deacetylase Inhibitors Research
- Muscle Physiology and Disorders
- Plant Surface Properties and Treatments
- Endometriosis Research and Treatment
Fudan University
2017-2024
South Central Minzu University
2017-2019
South China Agricultural University
2019
Yale University
2009-2013
Center for Vascular Biology Research
2011
MRC Laboratory of Molecular Biology
2008
Chinese Academy of Sciences
1999-2006
Center for Excellence in Molecular Cell Science
1999-2006
Shanghai Institutes for Biological Sciences
2000-2006
University of Oklahoma Health Sciences Center
2005
In canonical Wnt signaling, Dishevelled (Dvl) is a critical cytoplasmic regulator that releases β-catenin from degradation. Here, we find Dvl and c-Jun form complex with β-catenin–T-cell factor 4 (TCF-4) on the promoter of target genes regulate gene transcription. The forms via two interactions nuclear β-catenin, respectively, both which bind to TCF. Disrupting interaction either or suppresses signaling–stimulated transcription, reduction diminished β-catenin–TCF-4 association promoters in...
mTORC2 (mammalian target of rapamycin complex 2) plays important roles in signal transduction by regulating an array downstream effectors, including protein kinase AKT. However, its regulation upstream regulators remains poorly characterized. Although phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P(3)) is known to regulate the phosphorylation AKT Ser(473), hydrophobic motif (HM) site, mTORC2, it not clear whether PtdIns(3,4,5)P(3) can directly activity. Here, we used two...
The nuclear translocation of YAP1 is significantly implicated in the proliferation, stemness, and metastasis cancer cells. Although molecular basis underlying subcellular distribution has been extensively explored, it remains to be elucidated how localization signal guides pass through pore complex. Here, we define a globular type composed folded WW domains, named as WW-NLS. It directs import heterodimeric transport receptors KPNA−KPNB1, bypassing canonical that well documented...
Poly-ubiquitination-mediated RUNX2 degradation is an important cause of age- and inflammation-related bone loss. NEDD4 family E3 ubiquitin protein ligases are thought to be the major regulators poly-ubiquitination. However, we observed a mono-ubiquitination that was catalyzed by WWP2, member ligases. WWP2 has been reported catalyze Goosecoid in chondrocytes, facilitating craniofacial skeleton development. In this study, found osteogenic differentiation mesenchymal stem cells promoted...
There are a large number of Rho guanine nucleotide exchange factors, most which have no known functions. Here, we carried out short hairpin RNA-based functional screen Rho-GEFs for their roles in leukocyte chemotaxis and identified Arhgef5 as an important factor macrophage phage-like RAW264.7 cell line. can strongly activate RhoA RhoB weakly RhoC RhoG, but not Rac1, RhoQ, RhoD, or RhoV, transfected human embryonic kidney 293 cells. In addition, Gβγ interacts with stimulate Arhgef5-mediated...
Wnt/beta-catenin signaling plays a critical role in embryonic myogenesis. Here we show that, P19 carcinoma stem cells, initiates the myogenic process depends on beta-catenin-mediated relief of I-mfa (inhibitor MyoD Family a) suppression regulatory factors (MRFs). We found that beta-catenin interacted with and interaction was enhanced by Wnt3a. In addition, between able to attenuate MRFs, relieve I-mfa-mediated transcriptional activity cytosolic sequestration initiate myogenesis model system...
Two new Tricholoma terpenoids, tricholopardins A and B, were isolated from the fruiting bodies of basidiomycetes pardinum. Their structures elucidated by spectroscopic methods, as well electronic circular dichroism optical rotatory dispersion calculations. Tricholopardin potently inhibited nitric oxide production in lipopolysaccharide-induced RAW264.7 macrophages with an IC50 0.08 μM. Its anti-inflammatory effects on three inflammatory mediators also evaluated. plausible biosynthetic pathway...
Osteocalcin has recently been shown to regulate energy homeostasis through multiple pathways. Adipose tissue is a main organ of metabolism, and administration recombinant osteocalcin in mice promoted consumption, thus counteracting obesity glucose intolerance. The regulation islet β cells well documented; however, it unknown whether can also act on adipocytes and, if does, how functions. Here, we provide evidence demonstrate specific role for brown adipocyte thermogenesis. Importantly,...
Loquat (Eriobotrya japonica) is a subtropical tree bearing fruit that ripens during late spring and early summer, which the off-season for production. The specific flowering habit of loquat, starts in fall ends winter, has attracted an increasing number researchers who believe it may represent ideal model studying shift adaptations to climate change Rosaceae. These studies require understanding gene expression patterns within other tissues this plant. Although ACTINs (ACTs) have previously...
In the past decade, cleavage protein irisin derived from fibronectin type III domain-containing 5 (FNDC5) in exercise-stimulated skeletal muscle has increasingly become a biomarker associated with metabolic syndrome and osteoporosis humans. However, it is unclear how this facilitates muscle-adipose-bone connectivity homeostasis. study, we unexpectedly observed that FNDC5 gene can be markedly activated during differentiation of brown adipocytes but not white adipocytes, specifically expressed...
We previously characterized a Gα12-specific signaling pathway that stimulates the transcription of E3 ligase RFFL via protein kinase ARAF and ERK. This leads to persistent PKC activation is important for sustaining fibroblast migration. However, questions remain regarding how Gα12 specifically activates ARAF, which factor involved in Gα12-mediated expression, whether cell migration stimulated by other mechanisms can activate In this study, we show replacement residue Arg-264 with Gln,...
To investigate functions of the consensus amino terminus G protein-coupled receptor kinases (GRKs), two terminus-truncated mutants (delta30 or delta15) and single-amino-acid conserved acidic residues (D2A E7A) human GRK1 were constructed expressed in embryonic kidney 293 cells. It was shown that truncated mutations one single-point mutation (E7A) greatly decreased GRK1's activity to phosphorylate photoactivated rhodopsin (Rho*), whereas abilities these a synthetic peptide substrate...
G protein-coupled receptor kinases (GRKs) are a family of serine/threonine that phosphorylate many activated receptors (GPCRs) and play an important role in GPCR desensitization. Our previous work has demonstrated the C-terminal conserved region (CC) GRK-2 participates interaction with rhodopsin this is necessary for GRK-2-mediated phosphorylation (Gan, X. Q., Wang, J. Y., Yang, Q. H., Li, Z., Liu, F., Pei, G., L. (2000) Biol. Chem. 275, 8469-8474). In report, we further investigated whether...
We have previously shown that beta-catenin interacts with a transcription suppressor I-mfa and, through this interaction, canonical Wnt signaling could relieve I-mfa-mediated suppression of myogenic regulatory factors (MRFs). In study, we found that, based on the factor T-cell factor/lymphoid enhancing factor-1 (TCF/LEF-1) can also be relieved. Our work showed knocking down endogenous expression mimics treatment by inducing myogenesis and increasing reporter gene activity, target MRFs in P19...
Three new podocarpane diterpenoids, namely anemhupehins A-C (1-3), together with four known analogues (4-7), have been isolated from aerial parts of Anemone hupehensis. Their structures were characterized based on extensive spectroscopic data. Compounds 1 and 4 showed certain cytotoxicities against human cancer cell lines.
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