A5011058303- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- ATP Synthase and ATPases Research
- Mass Spectrometry Techniques and Applications
- Alzheimer's disease research and treatments
- Photosynthetic Processes and Mechanisms
- Metabolism and Genetic Disorders
- Biotin and Related Studies
- Fatty Acid Research and Health
- Radiomics and Machine Learning in Medical Imaging
- Parkinson's Disease Mechanisms and Treatments
- Neuroblastoma Research and Treatments
- RNA and protein synthesis mechanisms
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Signaling Pathways in Disease
- Gene expression and cancer classification
- Neuroscience and Neural Engineering
- Artificial Intelligence in Healthcare and Education
- Ion channel regulation and function
- Glycosylation and Glycoproteins Research
- COVID-19 Clinical Research Studies
- TGF-β signaling in diseases
- Single-cell and spatial transcriptomics
- Advanced Biosensing Techniques and Applications
Ruhr University Bochum
2012-2025
Technische Universität Dresden
2025
University Hospital Carl Gustav Carus
2025
University Hospital Frankfurt
1995-2004
Goethe University Frankfurt
1995-2004
Klinik und Poliklinik für Urologie
1997
Friedrich Schiller University Jena
1997
Cardiolipin stabilized supercomplexes of Saccharomyces cerevisiae respiratory chain complexes III and IV (ubiquinol:cytochrome c oxidoreductase cytochrome oxidase, respectively), but was not essential for their formation in the inner mitochondrial membrane because they were found also a cardiolipin-deficient strain. Reconstitution with cardiolipin largely restored wild-type stability. The putative interface comprises transmembrane helices cytochromes b c1 tightly bound cardiolipin. Subunits...
The ratios of the oxidative phosphorylation complexes NADH:ubiquinone reductase (complex I), succinate:ubiquinone II), ubiquinol:cytochrome c III), cytochrome oxidase IV), and F1F0-ATP synthase V) from bovine heart mitochondria were determined by applying three novel independent approaches that gave consistent results: 1) a spectrophotometric-enzymatic assay making use differential solubilization II III parallel assays spectra catalytic activities in samples before after ultracentrifugation...
Vacuolar proton-translocating ATPase (holoATPase and free membrane sector) was isolated from bovine chromaffin granules by blue native polyacrylamide gel electrophoresis. A 5-fold excess of sector over holoenzyme determined in granule membranes. M9.2, a novel extremely hydrophobic 9.2-kDa protein comprising 80 amino acids, detected the sector. It shows sequence structural similarity to Vma21p, yeast required for assembly vacuolar ATPase. second sector-associated (M8-9) identified...
Stable supercomplexes of bacterial respiratory chain complexes III (ubiquinol:cytochrome c oxidoreductase) and IV (cytochrome oxidase) have been isolated as early 1985 (Berry, E. A., Trumpower, B. L. (1985) J. Biol. Chem. 260, 2458-2467). However, these assemblies did not comprise complex I (NADH:ubiquinone oxidoreductase). Using the mild detergent digitonin for solubilization Paracoccus denitrificans membranes we could isolate NADH oxidase, assembled from I, III, in a 1:4:4 stoichiometry....
Currently data-dependent acquisition (DDA) is the method of choice for mass spectrometry-based proteomics discovery experiments, but data-independent (DIA) steadily becoming more important. One most important requirements to perform a DIA analysis availability suitable spectral libraries peptide identification and quantification. Several studies were performed addressing evaluation library performance protein in measurements. But so far only few experiments estimate effect these on...
Background: Polyunsaturated fatty acids in particular omega-3 acids, such as docosahexaenoic acid (DHA), are essential nutrients and components of the plasma membrane. They involved various processes, including synaptic development, functionality, integrity, plasticity, therefore thought to have general neuroprotective properties. Considerable research evidence further supports beneficial effects specifically on mitochondria, through their antioxidant anti-apoptotic properties, making them...
Evidence has shown that protein supplementation following resistance exercise training (RET) helps to further enhance muscle mass and strength. Studies have demonstrated collagen peptides containing mostly non-essential amino acids increase fat-free (FFM) strength in sarcopenic men. The aim of this study was investigate whether peptide combination with RET influences the composition skeletal muscle. Twenty-five young men (age: 24.2 ± 2.6 years, body (BM): 79.6 5.6 kg, height: 185.0 5.0 cm,...
Analysis of cerebrospinal fluid (CSF) is important for diagnosis neurological diseases. Especially neurodegenerative diseases, abnormal protein abundance in CSF an biomarker. However, the quality a key factor analytic outcome. Any external contamination has tremendous impact on analysis and reliability results. In this study, we evaluated effect blood with respect to biomarker identification. We compared three distinct measures: Combur10-Test® strips, specific hemoglobin ELISA, bottom-up...
The subunit composition of the mitochondrial ATP synthase from Saccharomyces cerevisiae was analyzed using blue native gel electrophoresis and high resolution SDS-polyacrylamide electrophoresis. We report here identification a novel molecular mass 6,687 Da, termed j (Su j). An open reading frame 127 base pairs (ATP18), which encodes for Su j, identified on chromosome XIII. does not display sequence similarity to subunits other organisms. Data searches, however, potential homolog...
Rapid technological advancements have made it possible to generate single-cell data at a large scale. Several laboratories around the world can now transcriptomic from different tissues. Unsupervised clustering, followed by annotation of cell type identified clusters, is crucial step in analyses. However, there no consensus on marker genes use for annotation, and celltype currently mostly done manual inspection genes, which irreproducible, poorly scalable. Additionally, patient-privacy also...
The yeast F1F0-ATP synthase forms dimeric complexes in the mitochondrial inner membrane and a manner that is supported by F0-sector subunits, Su e g. Furthermore, it has recently been demonstrated binding of F1F0-ATPase natural inhibitor protein to purified bovine F1-sectors can promote their dimerization solution (Çabezon, E., Arechaga, I., Jonathan P., Butler, G., Walker J. E. (2000) Biol. Chem. 275, 28353–28355). It was unclear until now whether F1 domains contributes process intact...
Cytochrome -c oxidase subunit VIaH (COXVIaH) has been implicated in the modulation of COX activity. A gene-targeting strategy was undertaken to generate mice that lacked COXVIaH determine its role regulation oxidative energy production and mechanical performance cardiac muscle. Total activity decreased hearts from mutant mice, which appears be a consequence altered assembly holoenzyme COX. However, total myocardial ATP not significantly different wild-type mice. Myocardial examined using...
LUHMES cells share many characteristics with human dopaminergic neurons in the substantia nigra, cells, demise of which is responsible for motor symptoms Parkinson's disease (PD). can, therefore, be used bona fide as a model to study pathophysiological processes involved PD. Previously, we showed that degenerate after 6 days upon overexpression wild-type alpha-synuclein. In present study, performed transcriptome and proteome expression analysis alpha-synuclein-overexpressing GFP-expressing...
Recent studies investigated the potential of large language models (LLMs) for clinical decision making and answering exam questions based on text input. developments LLMs have extended these with vision capabilities. These image processing are called vision-language (VLMs). However, there is limited investigation applicability VLMs their capabilities content. Therefore, aim this study was to examine performance publicly accessible in 2 different surgical question sets consisting questions....
Central hallmark of Alzheimer's disease are senile plaques mainly composed β-amyloid, which is a cleavage product the amyloid precursor protein (APP). The physiological function APP and its family members APLP1 APLP2 poorly understood. In order to fill this gap, we established cell-culture based model with simultaneous knockdown all family. A comprehensive proteome study APP/APLP1/APLP2 cell lysates versus controls revealed significant abundance changes more than 30 proteins. Targeted...
The amount of oxidative phosphorylation enzymes in mitochondrial encephalomyopathy patients has been studied by two‐dimensional electrophoresis (blue native PAGE/Tricine‐SDS‐PAGE). Only 20 mg muscle was required to identify and analyse complexes I, III, IV, V after Coomassie staining. In most cases reduced amounts the involved complex(es) correlated well with decreased enzyme activities. reliability method reflected constant mutual ratio found all controls. Deviations from normal ratios were...
The TrkA and TrkB tyrosine kinases are members of the neurotrophin receptor family mediate survival, differentiation, growth, apoptosis neurons in response to stimulation by their ligands, NGF BDNF, respectively. Expression levels TrkA/TrkB important prognostic factors a variety embryonal tumors including neuroblastoma, most common solid tumor childhood. Because exhibit high level sequence similarity use overlapping pathways for signal transduction, existence specific effector molecules...
The intracellular domain of the amyloid precursor protein (AICD) is generated following cleavage by γ-secretase complex and involved in membrane to nucleus signaling, for which binding AICD adapter FE65 essential. Here we show that knockdown causes a down regulation BLM MCM family elevated nuclear levels result stabilization mobile spheres. These spheres are able grow fuse, potentially correspond 10. plays role DNA replication repair mechanisms was also shown play cell's response damage. A...
The amount of oxidative phosphorylation enzymes in mitochondrial encephalomyopathy patients has been studied by two-dimensional electrophoresis (blue native PAGE/Tricine-SDS-PAGE). Only 20 mg muscle was required to identify and analyse complexes I, III, IV, V after Coomassie staining. In most cases reduced amounts the involved complex(es) correlated well with decreased enzyme activities. reliability method reflected constant mutual ratio found all controls. Deviations from normal ratios were...
A two-dimensional electrophoretic technique combining blue native polyacrylamide gel electrophoresis (BN-PAGE) with Tricine sodium dodecyl sulfate (SDS)-PAGE was previously used for the localization of oxidative phosphorylation (OXPHOS) defects in human diseases starting from biopsy or autopsy tissues (Schägger, H., Electrophoresis 1995, 16, 763-770). In present work extended resolution OXPHOS enzymes platelets and tissue-cultured cells. Silver staining is required to detect protein subunits...
Abstract The Human Proteome Organisation Brain Project aims at coordinating neuroproteomic activities with respect to analysis of development, aging, and evolution in human mice analysing normal aging processes as well neurodegenerative diseases. Our group participated the mouse pilot study this project using two different 2‐DE systems, find out optimal conditions for comprehensive gel‐based differential proteome analysis. Besides assessment best methodical question “How many biological...