Objective: Research suggests that Puerarin may protect against sepsis-induced myocardial damage. However, the mechanisms responsible for Puerarin’s cardioprotective effect remain largely unclear. In this study, our objective is to investigate role of Puerarin-induced AMPK-mediated ferroptosis signaling in protecting injury. Methods: 48 male Sprague-Dawley rats were randomly divided into four groups: control group, LPS + Pue Era (Erastin, activator) or CC (compound C, AMPK inhibitor) group....

Background and objective Stress hyperglycemia is common in critically ill patients associated with poor prognosis. Whether this association exists pulmonary hypertension (PH) unknown. The present cohort study investigated the of stress 90-day all-cause mortality intensive care unit (ICU) PH. Methods Data population were extracted from Medical Information Mart for Intensive Care IV (MIMIC-IV) database. A new index, ratio admission glucose to HbA1c (GAR), was used evaluate hyperglycemia....

The distinct honeybee (Apis mellifera) worker and queen castes have become a model for the study of genomic mechanisms phenotypic plasticity. Here we performed nanopore-based direct RNA sequencing with exceptionally long reads to compare mRNA transcripts between workers at three points during their larval development. We found thousands significantly differentially expressed transcript isoforms (DEIs) larvae. These DEIs were formatted by flexible splicing system. showed that poly(A) tails...

This study aims to evaluate the impact of colony stimulating factor-1 (CSF-1) expression on recurrence and survival patients with clear-cell renal cell carcinoma (ccRCC) following surgery.We retrospectively enrolled 267 (195 in training cohort 72 validation cohort) ccRCC undergoing nephrectomy at a single institution. Clinicopathologic features, cancer-specific (CSS) recurrence-free (RFS) were recorded. CSF-1 levels assessed by immunohistochemistry tumor tissues. Kaplan-Meier method was...

Activated Cdc42-associated kinase1 (ACK1), a non-receptor tyrosine kinase, has been considered as an oncogene and therapeutic target in various cancers. However, its contribution to cancer immunity remains uncertain. Here, we first compared the profiles of immune cells cancerous normal tissues The Cancer Genome Atlas (TCGA) lung cohorts. Next, found that cell infiltration levels were associated with ACK1 gene copy numbers cancer. Consistently, our RNA-seq data unveiled silencing upregulated...

Tumor-associated macrophages (TAMs) are the most prominent immune cell population in glioblastoma (GBM) tumor microenvironment (TME) and play critical roles promoting progression immunosuppression. Here we identified that TAM-derived legumain (LGMN) exhibited a dual role regulating biology of TAMs GBM cells. LGMN promoted macrophage infiltration cell-autonomous manner by activating GSK3b-STAT3 pathway. Moreover, activated integrin aV-AKT-P65 signaling to drive proliferation survival....

Abstract Background Epidemiological studies indicate that some characteristics of lung cancer among never-smokers significantly differ from those smokers. Aberrant promoter methylation and mutations in oncogenes tumor suppressor genes are frequent tumors smokers but rare never-smokers. In this study, we analyzed the ras-association domain isoform A (RASSF1A) death-associated protein kinase (DAPK) patients with primarily non-small cell (NSCLC) Western Pennsylvania region. We compare results...

<b><i>Introduction:</i></b> Obesity has been believed to be closely linked with many kinds of diseases including atherosclerosis, hypertension, cerebrovascular thrombosis, and diabetes. Ghrelin Homeobox transcript antisense RNA (HOTAIR) were involved in the regulation myocardial injury. <b><i>Methods:</i></b> The obesity mice model was established through feeding (C57BL/6J, male, eight-week-old) high-fat diet palmitate (PA)-induced...

Although the use of pleural effusion samples for epidermal growth factor receptor (EGFR) testing in lung cancer is increasing, accuracy rate and effectiveness identifying EGFR mutations using these samples, rather than primary tumor tissue not established.One hundred ninety-two advanced, non-small cell patients were enrolled into this study. All had corresponding we employed Amplification Refractory Mutation System to detect gene samples.The number detected was 119 (61.98%) 113 (58.85%),...

Carboxypeptidase E (CPE) is one of the most important carboxypeptidases involved in biosynthesis numerous peptide hormones and neurotransmitters has an role endocrine regulation. A splice variant CPE (CPE-ΔN) been detected mechanism CPE-ΔN action tumorigenesis studied many different cancers. The aim this study was to examine expression human colorectal cancer (CRC) evaluate its possible use as a potential prognostic marker. Two hundred nineteen primary tumors corresponding normal tissues...

Lung cancer is the leading cause of death in patients. Based on a modular and comprehensive analysis method, it intended to identify their common pathogenesis. We downloaded data analyzed differences lung adenocarcinoma samples, squamous cell carcinoma normal samples. Co-expression analysis, enrichment hypergeometric testing were used predict transcription factors, ncRNAs, retrospective target genes. get 4596 differentially expressed genes high multiples clustered into 14 modules...

To evaluate the effects of ZnO NPs on bone growth in rats and explore possible mechanisms action.Three-week-old male received ultrapure water or 68, 203, 610 mg/kg zinc oxide nanoparticles (ZnO NPs) for 28 days, orally.The high-dosage groups caused significant differences weight rate, body length, tibia length (P<0.05), all decreasing with increased NP dosage. There were no mass index (BMI) (P>0.05). The concentration liver tissue significantly dosage (P<0.05). Clearly aspartate...

This study aimed to determine the functional relationship between levels of dachshund homolog 1 (DACH1) expression and different subtypes ovarian cancer investigate possible prognostic value DACH1 in cancer.Immunohistochemical staining was deployed protein DACH1. Staining performed on patient samples, for whom detailed follow-up data have been acquired during last 10 years. Normal, benign, borderline, cancer, metastatic samples were included this study.The results our show that increase with...

Objective: To explore three novel fasting blood glucose (FBG)-based indicators, including FBG-to-albumin ratio (FAR), FBG-to-lymphocytes (FLR), and FBG-to-hemoglobin (FHR), in predicting prognosis detecting metastasis for patients with pancreatic neuroendocrine neoplasms after resection. Materials methods: A total of 178 pNEN underwent surgical resection were included this study. Receiver operating characteristic (ROC) curves used to evaluate the diagnosis values FAR, FLR, FHR, cut-off...