A5004610453- Analytical Chemistry and Chromatography
- Analytical chemistry methods development
- Mass Spectrometry Techniques and Applications
- Pharmaceutical and Antibiotic Environmental Impacts
- Advanced Proteomics Techniques and Applications
- Antibiotic Resistance in Bacteria
- Microfluidic and Capillary Electrophoresis Applications
- Electrochemical Analysis and Applications
- Evolution and Genetic Dynamics
- Antimicrobial Resistance in Staphylococcus
- Chemical Synthesis and Analysis
- Atmospheric chemistry and aerosols
- Pharmacological Effects and Assays
- X-ray Diffraction in Crystallography
- Analytical Chemistry and Sensors
- Bacterial Identification and Susceptibility Testing
- Crystallization and Solubility Studies
- Click Chemistry and Applications
- Electrocatalysts for Energy Conversion
- Protein purification and stability
- Dietary Effects on Health
- Bioinformatics and Genomic Networks
- Protein Structure and Dynamics
- Cannabis and Cannabinoid Research
- Complex Systems and Decision Making
University of Bath
2012-2021
Applied Mathematics (United States)
2015
Engineering and Physical Sciences Research Council
2015
Bruker (United Kingdom)
2004-2006
This is the first study presenting a multi-residue method allowing for comprehensive analysis of several chiral pharmacologically active compounds (cPACs) including beta-blockers, antidepressants and amphetamines in wastewater digested sludge at enantiomeric level. Analysis both liquid solid matrices within treatment crucial to being able carry out mass balance these systems. The developed comprises filtration, microwave assisted extraction phase followed by chromatography coupled with...
It is now apparent that each of the known, naturally occurring polyphosphoinositides, phosphatidylinositol monophosphates (PtdIns3P, PtdIns4P, PtdIns5P), bisphosphates [PtdIns(3,4)P(2), PtdIns(3,5)P(2), PtdIns(4,5)P(2)], and trisphosphate [PtdIns(3,4,5)P(3)], have distinct roles in regulating many cellular events, including intracellular signaling, migration, vesicular trafficking. Traditional identification techniques require [(32)P]inorganic phosphate or [(3)H]inositol radiolabeling,...
Here presented for the first time is enantioselective biodegradation of amphetamine and methamphetamine in river microcosm bioreactors. The aim this investigation was to test hypothesis that mechanisms governing fate environment are mostly stereoselective biological nature. Several bioreactors were studied over duration 15 days (i) both biotic abiotic conditions, (ii) dark or exposed light (iii) presence absence suspended particulate matter. Bioreactor samples analysed using...
The study of highly air- and moisture-sensitive organometallic complexes by ESI-MS is greatly facilitated interfacing the instrument to an inert-atmosphere glovebox, a modification that requires no special precautions beyond proximity.
Neutrophil influx into the intestinal lumen is a critical response to infectious agents, but also associated with severe damage observed in idiopathic inflammatory bowel disease. The chemoattractant hepoxilin A3, an eicosanoid secreted from epithelial cells by apically restricted efflux pump multidrug resistance protein 2 (MRP2), mediates this neutrophil influx. Information about possible counterbalance pathway that could signal lack of or resolution apical signal, however, has yet be...
Fitness costs imposed on bacteria by antibiotic resistance mechanisms are believed to hamper their dissemination. The scale of these is highly variable. Some, including Staphylococcus aureus the clinically important mupirocin, have been reported as being cost-free, which suggests that there few barriers preventing global spread. However, this not supported surveillance data in healthy communities, indicate mechanism relatively unsuccessful. Epistasis analysis two collections MRSA provides an...
Abstract. The composition of organic aerosol formed from the gas phase ozonolysis cyclohexene has been investigated in a smog chamber experiment. Comprehensive chromatography with time flight mass spectrometric detection was used to determine that dicarboxylic acids and corresponding cyclic anhydrides dominated small reaction products found sampled during first hour after initial formation. Structural analysis larger more polar molecules performed using liquid ion trap tandem spectrometry....
Abstract Liquid chromatography combined with electrospray ionization mass spectrometry (LC/ESI‐MS) has been used successfully for the characterization of biomolecules in proteomics last few years. This methodology relied largely on use reversed‐phase chromatography, particular C18‐based resins, which are suitable separation peptides. Here we show that polymeric [polystyrene divinylbenzene] monolithic columns can be to separate peptide mixtures faster and at a higher resolution. For 500 fmol...
Product ion spectra produced by collision-induced dissociation (CID) in tandem mass spectrometry experiments can differ markedly between instruments. There have been a number of attempts to standardise the production product spectra; however, consensus on most appropriate approach reproducible has yet be reached. We previously reported comparison different types instruments - triple quadrupole, two traps and Fourier transform cyclotron resonance spectrometer (Bristow AWT, Webb KS, Lubben AT,...
Abstract The results of the comparison product‐ion tandem mass (MS/MS) spectra recorded on three ion trap spectrometers, a triple quadrupole spectrometer and Fourier transform cyclotron resonance are reported. were in accordance with simple experimental protocol, which involved collision‐induced dissociation (CID) attenuation abundance [M+H] + to between 10 50% its original abundance. degree similarity from four spectrometers was calculated off‐line by comparing five most abundant ions...
Deoxyribose-1-phosphate (dRP) is a proangiogenic paracrine stimulus released by cancer cells, platelets, and macrophages acting on endothelial cells. The objective of this study was to clarify how dRP stimulates angiogenic responses in human Live cell imaging, electron paramagnetic resonance, pull-down dRP-interacting proteins, followed immunoblotting, gene silencing different NADPH oxidases (NOXs), their regulatory cosubunits small interfering RNA (siRNA) transfection, experiments with...
Interactions between naturally occurring proteins are highly specific, with protein-network imbalances associated numerous diseases. For designed protein-protein interactions (PPIs), required specificity can be notoriously difficult to engineer. To accelerate this process, we have derived peptides that form heterospecific PPIs when combined. This is achieved using software generates large virtual libraries of peptide sequences and searches within the resulting interactome for preferentially...
An enzyme-triggered catalytic signal amplification cascade is described through the design of a novel enzyme substrate that selectively activates an organometallic transfer hydrogenation catalyst once triggered.
Abstract This manuscript presents the development, validation and application of a multi-residue supercritical fluid chromatography coupled with tandem mass spectrometry method for analysis 140 chiral non-chiral chemicals emerging concern in environmental samples, 81 compounds being fully quantitative, 14 semi-quantitative 45 qualitative, validated according to European Medicine Agency (EMA) guidelines (European Medicines 2019). One unified LC-MS was used analyse all analytes, which were...
Selective bioconjugation remains a significant challenge for the synthetic chemist due to stringent reaction conditions required by biomolecules coupled with their high degree of functionality. The current trailblazer transition-metal mediated chemistry involves use Pd(II) complexes prepared via an oxidative addition process. Herein, preparation cysteine facile C-H activation process is reported. These show efficiency competitive what seen in literature, user-friendly synthesis, common...
Two types of generator-collector electrode systems, (i) a gold-gold interdigitated microband array and (ii) dual-plate microtrench, are compared for nitrobenzene electroanalysis in aerated aqueous 0.1 M NaOH. The complexity the reduction conjunction with presence ambient levels oxygen analysis solution provide challenging problem which feedback-amplified steady state currents analytical signal. In contrast to more openly accessible geometry electrode, where voltammetric response is less...
Abstract Liquid|liquid interfaces provide a natural boundary and reactive interface where an organic phase is in contact with aqueous analyte. The selectivity of ion transfer processes at liquid|liquid can help to sensitivity, introduce reagents, or allow analyte accumulation the electrode surface. In this study, microdroplet deposits liquid 4‐(3‐phenylpropyl)‐pyridine (PPP) ferrocenylmethyl‐dodecyldimethylammonium + (FDA ) redox system are deposited onto basal plane pyrolytic graphite...
Peter J. Schoenmakers opened discussion of the paper by Ljiljana Paša-Tolić: You state as your objective that you want to achieve spatial resolution on a patch soil bringing sample size down 1 mm3. However, given time required for extraction and (LC) separation, wouldn't it req
The fraction of intact monomer in a sample (moles/moles), the monomeric purity, is measured as quality control therapeutic monoclonal antibodies but often unknown research samples and remains major source variation quantitative antibody-based techniques such immunoassay development. Here, we describe novel multiplex technique for estimating purity antigen affinity grade antibody samples. Light scattering was used to simultaneously observe mass binding biosensor surfaces functionalised with...
An electrochemical flow cell with a boron-doped diamond dual-plate microtrench electrode has been developed and demonstrated for hydroquinone injection electroanalysis in phosphate buffer pH 7. Using the generator-collector feedback detector improves sensitivity by one order of magnitude (when compared to single working detector). The diffusion process is switched from an analyte consuming "external" regenerating "internal" benefits selectivity sensitivity.
Illumination of visible light photocatalytic water oxidations using continuous or intermittent was investigated a range earth abundant metal oxides. Brief cessations allowed reduced harvester degradation and gave an approximate doubling yield reaction rate.
Gianluca Trifirò opened discussion of the paper by Johan Trygg: With regard to multivariate analysis data with different sizes, structures and sources, could kind transformation on raw affect final distribution, possibly misleading interpretation correlatio