A5058053423- Parasitic Infections and Diagnostics
- Coccidia and coccidiosis research
- Helminth infection and control
- Amoebic Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Meat and Animal Product Quality
- Parasites and Host Interactions
- Pharmacological Effects and Assays
South China Agricultural University
2023-2024
China Agricultural University
2018-2020
Eimeria species are pathogenic protozoa with a wide range of hosts and the cause poultry coccidiosis, which results in huge economic losses to industry. These parasites encode genome ∼8000 genes that control highly coordinated life cycle asexual replication sexual differentiation, transmission, virulence. However, function physiological importance large majority these remain unknown mostly due lack tools for systematic analysis gene functions. Here, we report first application CRISPR-Cas9...
Biliary cryptosporidiosis caused by Cryptosporidium is life-threatening in immunocompromised individuals, particularly those with acquired immune deficiency syndrome (AIDS), resulting AIDS cholangiopathy. However, studies of biliary have lagged due to the lack vitro models that allow complete pathogen development. Here we describe procedures for generation mouse cholangiocyte organoids (Chol-orgs). The Chol-orgs, which expressed stem and cell markers, could be clonally expanded three months...
Chicken coccidiosis, caused by the infection of Eimeria species, leads to important economic losses poultry industry. Vaccination with attenuated live parasites seems be best way control this disease. Attenuated eimerian shortened prepatent times show great changes in intracellular development compared their parent strains but mechanisms involved these biological differences are still unclear. In study, we obtained a precocious line E. maxima sequential selection 22 generations early shed...
ABSTRACT Cryptosporidium hominis and parvum are major causes of severe diarrhea in humans. Comparative studies them hampered by the lack effective cultivation cryopreservation methods, especially for C. . Here, we described adapted murine enteroids one IId subtype nonhuman primate-adapted Ib, Im, In subtypes, which allowed complete development pathogens, producing oocysts infectious to mice. Using system, developed a novel method isolates. comparative RNA-seq analyses cultures, enteroid...
Cryptosporidium hominis and parvum are major causes of severe diarrhea in humans. Comparative studies them hampered by the lack effective cultivation cryopreservation methods, especially for C. hominis. Here, we described adapted murine enteroids one IId subtype nonhuman primate-adapted Ib, Im, In subtypes, which allowed complete development pathogens, producing oocysts infectious to mice. Using system, developed a novel method isolates. comparative RNA-seq analyses cultures, enteroid system...
A major bottleneck in the progress of Cryptosporidium research is lack accessible cryopreservation oocysts. Here, we present a protocol for isolates using enteroids. We describe steps establishment enteroid cultures and C. parvum-infected HCT-8 cultures. then detail procedures recovery propagation frozen parasites For complete details on use execution this protocol, please refer to Deng et al.1
Eimeria stiedae is one of the few eukaryotic pathogens that exclusively infect liver and serves as a good model to study host–pathogen interactions in this vital organ. In study, we show rabbits infected with E. develop severe but self‐healing cholangitis. RNA‐seq analysis gene expression landscapes over long course infection identified 912 differentially expressed genes (DEGs) prepatent period (794 up‐ 118 downregulated genes), 2889 DEGs early oocyst shedding (1870 1019 2859 peak (1923 936...