A5013040792- Redox biology and oxidative stress
- Photosynthetic Processes and Mechanisms
- Metal-Catalyzed Oxygenation Mechanisms
- BRCA gene mutations in cancer
- Genetic Associations and Epidemiology
- Metalloenzymes and iron-sulfur proteins
- Estrogen and related hormone effects
- Mass Spectrometry Techniques and Applications
- Glutathione Transferases and Polymorphisms
- HER2/EGFR in Cancer Research
- Genetic factors in colorectal cancer
- Mitochondrial Function and Pathology
- Cytokine Signaling Pathways and Interactions
- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Cervical Cancer and HPV Research
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Neuroscience of respiration and sleep
- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- Sulfur Compounds in Biology
- Hepatitis C virus research
Medizinische Hochschule Hannover
2016-2025
Boston Children's Hospital
2025
Utrecht University
2025
University of Manchester
2021
St Mary's Hospital
2021
Edinburgh Cancer Research
2021
Cancer Research Center
2021
University of Neuchâtel
2004-2018
Pomeranian Medical University
2015
Klinik für Frauenheilkunde
2014
In contrast to a detailed understanding of antiviral cellular immune responses, the impact neutralizing antibodies for resolution acute hepatitis C is poorly defined. The analysis responses has been hampered by fact that patient cohorts as well virus (HCV) strains are usually heterogeneous, and clinical data from acute-phase long-term follow-up after infection not readily available. Using an infectious retroviral HCV pseudoparticle model system, we studied cohort women accidentally exposed...
Thioredoxins are small multifunctional redox active proteins widely if not universally distributed among living organisms. In chloroplasts, two types of thioredoxins ( f and m ) coexist play central roles in regulating enzyme activity. Reduction chloroplasts is catalyzed by an iron-sulfur disulfide enzyme, ferredoxin-thioredoxin reductase, that receives photosynthetic electrons from ferredoxin, thereby providing a link between light Chloroplast function the regulation Calvin cycle associated...
A three-stage genome-wide association study recently identified single nucleotide polymorphisms (SNPs) in five loci (fibroblast growth receptor 2 (FGFR2), trinucleotide repeat containing 9 (TNRC9), mitogen-activated protein kinase 3 K1 (MAP3K1), 8q24, and lymphocyte-specific 1 (LSP1)) associated with breast cancer risk. We investigated whether the associations between these SNPs risk varied by clinically important tumor characteristics up to 23,039 invasive cases 26,273 controls from 20...
Thioredoxins, the ubiquitous small proteins with a redox active disulfide bridge, are important regulatory elements in plant metabolism. Initially recognized as reversible light activation of key photosynthetic enzymes, they have subsequently been found cytoplasm and mitochondria. The various thioredoxins different structure function. Depending on their intracellular location reduced enzymatically by an NADP-dependent or ferredoxin (light)-dependent reductase transmit signal to selected...
The prion protein (PrP C ) is a copper-binding of unknown function that plays an important role in the etiology transmissible spongiform encephalopathies. Using morphological techniques and synaptosomal fractionation methods, we show PrP predominantly localized to synaptic membranes. Atomic absorption spectroscopy was used identify -related changes copper concentration transgenic mouse lines. transmission presence H 2 O , which known be decomposed highly reactive hydroxyl radicals iron or...
Mitochondria contain thioredoxin (Trx), a regulatory disulfide protein, and an associated flavoenzyme, NADP/Trx reductase, which provide link to NADPH in the organelle. Unlike animal yeast counterparts, function of Trx plant mitochondria is largely unknown. Accordingly, we have applied recently devised proteomic approaches identify soluble Trx-linked proteins isolated from photosynthetic (pea spinach leaves) heterotrophic (potato tubers) sources. Application mitochondrial extracts mutant...
The 2-cysteine peroxiredoxins (2-Cys Prx) constitute an ancient family of peroxide detoxifying enzymes and have acquired a plant-specific function in the oxygenic environment chloroplast. Immunocytochemical analysis work with isolated intact chloroplasts revealed reversible binding oligomeric form 2-Cys Prx to thylakoid membrane. enzyme was enhanced under stress. has broad substrate specificity activity toward hydrogen peroxides complex alkyl hydroperoxides. During reduction reaction, is...
Abstract The hydrolytic cleavage of Pi from fructose diphosphate by the diphosphatase (FDPase) system spinach chloroplasts was found to require (a) an alkaline FDPase component insensitive inhibition AMP or diphosphate; (b) reduced ferredoxin; (c) a protein factor; and (d) Mg++. Reduced ferredoxin could be replaced methyl viologen dithiothreitol but not bacterial ferredoxin. Activation nonphysiological substitutes required factor. in isolated intact also activated light. factor has been...
A growing number of processes throughout biology are regulated by redox via thiol–disulfide exchange. This mechanism is particularly widespread in plants, where almost 200 proteins have been linked to thioredoxin (Trx), a widely distributed small regulatory disulfide protein. The current study extends regulation Trx amyloplasts, organelles prevalent heterotrophic plant tissues that, among other biosynthetic activities, catalyze the synthesis and storage copious amounts starch. Using...
Abstract A recent genome-wide association study found that genetic variants on chromosomes 3, 6, 7, 10, 11, 19 and X were associated with prostate cancer risk. We evaluated the most significant single-nucleotide polymorphisms (SNP) in these loci using a worldwide consortium of 13 groups (PRACTICAL). Blood DNA from 7,370 cases 5,742 male controls was analyzed by genotyping assays. Odds ratios (OR) each genotype estimated unconditional logistic regression. Six seven SNPs showed clear evidence...
Light generates reducing equivalents in chloroplasts that are used not only for carbon reduction, but also the regulation of activity chloroplast enzymes by reduction regulatory disulfides via ferredoxin:thioredoxin reductase (FTR) system. FTR, key electron/thiol transducer enzyme this pathway, is unique it can reduce an iron-sulfur cluster, a property explained tight contact its active-site disulfide and center. The thin, flat FTR molecule makes two-electron possible forming on one side...
The potato tuber (<i>Solanum tuberosum</i>L.) ADP-glucose pyrophosphorylase (ADP-GlcPPase) catalyzes the first committed step in starch biosynthesis. main type of regulation this enzyme is allosteric, and its activity controlled by ratio activator, 3-phosphoglycerate to inhibitor, P<sub>i</sub>. It was reported (Fu, Y., Ballicora, M. A., Leykam, J. F., Preiss, (1998) <i>J. Biol. Chem</i>. 273, 25045–25052) that activated reduction Cys<sup>12</sup>disulfide linkage present catalytic subunits....
To identify prognostic factors in medulloblastoma, a common malignant brain tumor of childhood, expression the oncogene c-myc was examined at mRNA level by situ hybridization. observed 30 72 tumors (42%). The gene copy number determined quantitative PCR from genomic DNA paraffin-embedded tumors. amplification present 5 62 cases (8.3%). Therefore, obviously not cause most samples. Kaplan-Meier estimation revealed significant correlation between and survival (total mean follow-up 4.6 +/- 3.6...
Oxidation-reduction midpoint potentials were determined, as a function of pH, for the disulfide/dithiol couples spinach and pea thioredoxins f, Chlamydomonas reinhardtii m, ferredoxin:thioredoxin reductase (FTR), two enzymes regulated by thioredoxin phosphoribulokinase (PRK) fructose-1,6-bisphosphatases (FBPase) from spinach. Midpoint oxidation-reduction potential (Em) values at pH 7.0 −290 mV both −300 C. −320 FTR, PRK, −315 FBPase, −330 FBPase obtained. With exception titrations showed...
The recently discovered potato tuber ( Solanum tuberosum ) α-glucan, water dikinase (GWD) (formerly known as R1) catalyzes the phosphorylation of starch by a dikinase-type reaction mechanism in which β-phosphate ATP is transferred to either C-6 or C-3 position glucosyl residue starch. In present study, we found that GWD enzyme inactive oxidized form, accompanied formation specific intramolecular disulfide bond determined disulfide-linked peptide mapping. regulatory properties this linkage...
Mutations in the NBS1 gene have been identified as disease-causing mutations patients with Nijmegen Breakage Syndrome (NBS), but their clinical impact on breast cancer susceptibility has remained uncertain. We determined frequency of 2 NBS mutations, 657del5 and R215W, two large series cases controls from Northern Germany Republic Belarus. The 5-bp-deletion was 15/1,588 (0.9%) Belarus 1/1,076 (0.1%) only 1/1,014 population 0/1017 German (p < 0.01). missense substitution R215W observed...