A5086999307- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Immune responses and vaccinations
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Brucella: diagnosis, epidemiology, treatment
- Salmonella and Campylobacter epidemiology
- Quinazolinone synthesis and applications
- Thallium and Germanium Studies
- Veterinary medicine and infectious diseases
- Ginseng Biological Effects and Applications
- vaccines and immunoinformatics approaches
- Urban Green Space and Health
- Korean Urban and Social Studies
- Cytokine Signaling Pathways and Interactions
- Galectins and Cancer Biology
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
- Probiotics and Fermented Foods
- Antibiotics Pharmacokinetics and Efficacy
International Vaccine Institute
2023-2024
Yonsei University
2014-2017
Seoul National University
2009-2013
Synthetic double-stranded RNA analogs recognized by Toll-like receptor 3 (TLR3) are an attractive adjuvant candidate for vaccines, especially against intracellular pathogens or tumors, because of their ability to enhance T cell and antibody responses. Although poly(I:C) is a representative dsRNA with potent adjuvanticity, its clinical application has been limited due heterogeneous molecular size, inconsistent activity, poor stability, toxicity. To overcome these limitations, we developed...
// Han-Gyu Choi 1 , Seunga Yong Woo Back Seungwha Paik Hye-Soo Park Sik Kim 2 Hongmin Seung Bin Cha Chul Hee Sung Jae Shin Hwa-Jung Department of Microbiology, and Medical Science, College Medicine, Chungnam National University, Daejeon, Republic Korea Institute for Immunology Immunological Diseases, Brain 21 PLUS Project Yonsei University Seoul, Correspondence to: Kim, email: hjukim@cnu.ac.kr Shin, sjshin@yuhs.ac Keywords: tuberculosis, DC maturation, toll-like receptor 4, multifunctional T...
Identification of vaccine target antigens (Ags) that induce Ag-specific Th1 immunity is the first step toward development a tuberculosis vaccine. Here, we evaluated Mycobacterium (Mtb) protein Rv3628, soluble inorganic pyrophosphatase, as and characterized molecular details its interaction with dendritic cells (DCs). Rv3628 activated DCs, increasing their expression cell surface molecules augmenting production TNF-α, IL-1β, IL-6, IL-12p70. mediated these effects by binding to TLR2 activating...
A better understanding of the kinetics accumulated immune cells that are involved in pathophysiology during Mycobacterium tuberculosis (Mtb) infection may help to facilitate development vaccines and immunological interventions. However, innate adaptive associated with pathogenesis Mtb their relationship virulence not clearly understood. In this study, we used a mouse model compare bacterial burden, inflammation aerogenic lung between laboratory-adapted strains (Mtb H37Rv H37Ra) K strain,...
Fifteen nonrepetitive ampicillin-resistant Salmonella spp. were identified among 91 sp. isolates during nationwide surveillance of in waste from 131 chicken farms 2006 and 2007. Additional phenotyping genetic characterization these 15 by using indicator cephalosporins demonstrated that resistance to ampicillin reduced susceptibility cefoxitin three was caused TEM-1 DHA-1 beta-lactamases. Plasmid profiling Southern blot analysis DHA-1-positive serovar Indiana previously reported unrelated...
In this study, we focused on determining the distribution and prevalence of major plasmid replicons in β-lactam-resistant Escherichia fergusonii Enterobacteriaceae animal human origin. A high degree variability multiple were observed among isolates. The IncF IncI1 most prevalent E. Salmonella enterica serovar Indiana isolated from swine poultry South Korea, respectively. presence broad-host-range such as IncN, IncA/C, IncHI1, IncHI2 that are associated with important virulence genes toxins...
We evaluated effect of enterotoxigenic Escherichia coli (ETEC) specific lytic phage CJ12 in ETEC infected pigs. Phage was mixed with feed at a ratio 1:1,000 (0.1%). One week after initially providing feed, pigs were challenged orally 1011 CFU and body weight, diarrhea score, bacterial PFU the feces measured. Pigs treated groups C (106 PFU/g) D (108 showed more resistance to due infection compared positive control group B on third day initial challenge. Moreover, during quantitation feces,...
Recent studies have demonstrated the therapeutic potential of mesenchymal stem cells (MSCs) for treatment acute inflammatory injury and bacterial pneumonia, but their applications in mycobacterial infections not been investigated. In this study, we use MSCs as a novel strategy against Mycobacterium abscessus (M. abscessus), which is most drug-resistant difficult-to-treat pathogen. The systemic intravenous injection only improved mouse survival also enhanced clearance lungs spleen....
Brucella abortus is a facultative intracellular bacteria that replicates within macrophage without producing any classical virulence factors. It can become internalized to cells by zipper-like and/or swimming internalization mechanisms. However, the bacterial proteins involved in remain unclear. To define these proteins, random insertion mutants of B. were generated Tn5 transposome complexes. In all, 132 screened, cellular internalization-defective selected, and genomic envelope proteomic...
The latency and reactivation of Mycobacterium tuberculosis infection has been well studied. However, there have few studies the avium complex (MAC), most common etiological non-tuberculous species next to M. in humans worldwide. We hypothesized that latent MAC infections can be reactivated following immunosuppression after combination chemotherapy with clarithromycin rifampicin under experimental conditions. To this end, we employed a modified Cornell-like murine model investigated six...
Adherence factors and enterotoxins are major virulence of enterotoxigenic Escherichia coli (ETEC). Antibiotics have been used frequently for the treatment prevention ETEC infection in piggeries worldwide, including Korea. Therefore, data on both profiles antibiotic resistance patterns useful epidemiological study ETEC. A total number 198 E. field isolates were examined. The most prevalent pathotype was F1, followed by a combination F1 EAST1. All 71 resistant to more than 2 antibiotics disk...
Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia, a highly contagious pulmonary disease in pigs with major economic losses for pig producers worldwide. Whereas A. isolates are divided into 15 serotypes, secrete 4 types exotoxins (ApxI, ApxII, ApxIII, and ApxIV), which known as virulence factors. In current study, ApxIA, ApxIIA, ApxIIIA genes were amplified their recombinant proteins expressed Escherichia coli M15 cells. The antigenicity each protein was...
Abstract Background Brucella abortus is an intracellular zoonotic pathogen which causes undulant fever, endocarditis, arthritis and osteomyelitis in human abortion infertility cattle. This bacterium able to invade replicate host macrophage instead of getting removed by this defense mechanism. Therefore, understanding the interaction between virulence bacteria cell important control brucellosis. Previously, we generated internalization defective mutants analyzed envelope proteins. The present...
Although Mycobacterium abscessus (M. abscessus) is becoming more prevalent in patients without overt immunodeficiency, little known about the factors that contribute to disease susceptibility. This study was undertaken investigate how Toll-like receptor 2 (TLR2) functionally contributes generation of protective immunity against M. a morphotype-specific manner. We found Tlr2-/- mice were extremely susceptible an intravenous (i.v.) model infection by rough variants, displaying uncontrolled...
Mycobacterium bovis bacillus Calmette-Guerin (BCG), the only licensed vaccine, shows limited protection efficacy against pulmonary tuberculosis (TB), particularly hypervirulent (Mtb) strains, suggesting that a logistical and practical vaccination strategy is urgently required. Boosting BCG-induced immunity may offer potentially advantageous for advancing TB vaccine development, instead of replacing BCG completely. Despite improved airway immunization by using live BCG, use as an boosting...
ABSTRACT Florfenicol resistance was analyzed in 230 enteric pig isolates collected between 1998 and 2006. PCR, plasmid profiling, Southern blot hybridization, a mixed-broth conjugation assay suggested the intra- interspecies plasmid-mediated transfer of florfenicol among that exhibited MICs for 4 to 128 mg/liter.
Swine hepatitis E virus (HEV) is widespread throughout pigs in both developing and industrialized countries. This an important zoonotic agent a public concern worldwide. Infected are asymptomatic, so diagnosing swine HEV relies on detection of the or antibodies against virus. However, several obstacles need to be overcome for effective practical serological diagnosis. In this study, we developed enzyme-linked immunosorbent assay (ELISA) that used purified recombinant capsid protein HEV. The...
Abstract Background The aim of this study was to analyze the significance leucine proline substitution at position 138(Leu138Pro) on hydrolysis penicillin and ampicillin that we identified in bla SHV gene clinical Escherichia coli swine isolate. Results Kinetic analysis mutant proteins showed K m value purified L138P comparatively higher than SHV-1, SHV-33 SHV-33(L138P) enzyme for ampicillin. Docking simulation SHV-1 SHV-(L138P) enzymes also confirmed β-lactamases preferred had a binding...
Tuberculosis (TB) is the second leading infectious cause of mortality worldwide with about two million deaths per year. The only licensed TB vaccine, Mycobacterium bovis bacillus Calmette-Guerin (BCG) shows limited protection efficacy suggesting an improved vaccination strategy required. Recently, several vaccine candidates have entered clinical trials. These are live mycobacterial vaccines designed to replace BCG or subunit boost immunity induced by BCG. Vaccines different such as...