A5100672329- Receptor Mechanisms and Signaling
- Hormonal Regulation and Hypertension
- Cancer-related molecular mechanisms research
- Nitric Oxide and Endothelin Effects
- Ion Transport and Channel Regulation
- Birth, Development, and Health
- Renin-Angiotensin System Studies
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Blood Pressure and Hypertension Studies
- Electrolyte and hormonal disorders
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Neuropeptides and Animal Physiology
- Pregnancy and preeclampsia studies
- Extracellular vesicles in disease
- Ophthalmology and Visual Impairment Studies
- Sodium Intake and Health
- Congenital heart defects research
- Acute Kidney Injury Research
- Lipoproteins and Cardiovascular Health
- Ion channel regulation and function
- Adenosine and Purinergic Signaling
- Genetics and Neurodevelopmental Disorders
Army Medical University
2016-2025
Daping Hospital
2016-2025
University of Chinese Academy of Sciences
2020-2025
Nanjing University of Posts and Telecommunications
2025
Shanghai Ninth People's Hospital
2025
Shanghai Jiao Tong University
2025
Shanghai Institute of Hematology
2025
Chongqing Medical University
2022-2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2024
Chinese PLA General Hospital
2024
Extracellular vesicles (EVs) carry signals within or at their limiting membranes, providing a mechanism by which cells can exchange more complex information than what was previously thought. In addition to mRNAs and microRNAs, there are DNA fragments in EVs. Solexa sequencing indicated the presence of least 16434 genomic (gDNA) EVs from human plasma. Immunofluorescence study showed direct evidence that acridine orange-stained EV DNAs could be transferred into localize inside nuclear...
Long non-coding RNAs (lncRNAs) have been reported to be involved in the pathogenesis of cardiovascular disease (CVD), but whether circulating lncRNAs can serve as a coronary artery (CAD), biomarker is not known. The present study screened by microarray analysis plasma from CAD patients and control individuals found that 265 were differentially expressed. To find specific possible candidates, we used following criteria for 174 up-regulated lncRNAs: signal intensity ≥8, fold change >2.5...
Long non-coding RNAs (lncRNAs) have been found to be involved in the pathogenesis of coronary artery disease (CAD). However, it remains established whether or not circulating lncRNAs can serve as biomarkers CAD. Using a microarray-based lncRNA expression profiling, we 86 that were differentially expressed peripheral blood monocytes and plasma from 15 CAD patients control subjects. After choosing consistent criterion (average normalized intensity ≥7 with significance <0.005) confirmed by...
Ischemic heart disease (IHD) is the leading cause of death among noncommunicable diseases worldwide, but data on current epidemiological patterns and associated risk factors are lacking.
Several studies have reported an association between enteric bacteria and atherosclerosis. Bacterial 16S ribosomal RNA (rRNA) gene belong to Enterobacteriaceae been detected in atherosclerotic plaques. How intestinal go into blood is not known. Zonulin reversibly modulate permeability (IP), the circulating zonulin levels were increased diabetes, obesity, all of which are risk factors for It unclear whether changed coronary artery disease (CAD) patients IP. The rRNA sample was checked by 454...
Vascular smooth muscle cells (VSMCs) phenotype switch from contractile to proliferative is a pathological hallmark in various cardiovascular diseases. Recently, subset of long noncoding RNAs was identified produce functional polypeptides. However, the impact and regulatory mechanisms VSMCs switching remain be fully elucidated.
Adverse environmental exposure during the prenatal period can lead to diseases in offspring, including hypertension. Whether or not hypertensive phenotype be transgenerationally transmitted is known.Pregnant Sprague Dawley rats were intraperitoneally injected with lipopolysaccharide (LPS) on gestation days 6, 8, 10, and 12 generate LPS model. Blood pressure was monitored by both telemetry tail-cuff method. RNA sequencing performed analyze transcriptome alteration kidney of third generation....
Aims: Smooth muscle cell (SMC) remodeling poses a critical feature in the development and progression of atherosclerosis.Although fate mapping silicon approaches have expanded SMC phenotypes atherosclerosis, it still remains elusive about contributions individual molecular dynamics to advanced atherosclerotic plaque.Methods: Using single-cell transcriptome, we investigated cellular compositions human carotid plaque laden with core, followed by vivo experiments utilizing SMC-lineage tracing...
The dopaminergic and renin angiotensin systems interact to regulate blood pressure. Disruption of the D(3) dopamine receptor gene in mice produces renin-dependent hypertension. In rats, D(2)-like receptors reduce II binding sites renal proximal tubules (RPTs). Because major RPTs is receptor, we examined whether type 1 (AT(1)) rat RPT cells. effect on AT(1) was studied vitro vivo. agonist PD128907 decreased protein mRNA WKY cells increased it SHR but had no D(3)/AT(1) colocalized cells;...
Long noncoding RNA (lncRNA) can regulate various physiological and pathological processes through multiple molecular mechanisms in cis trans. However, the role of lncRNAs cardiac hypertrophy is yet to be fully elucidated. A mouse lncRNA microarray was used identify differentially expressed hearts following transverse aortic constriction-induced pressure overload comparing sham-operated samples. The direct impact one lncRNA, Ahit, on cardiomyocyte characterized neonatal rat cardiomyocytes...
Background Insulin resistance is associated with atrial remodeling as well fibrillation (AF). However, there was limited evidence on the relationship of triglyceride-glucose index (TyG) index, a simple, valuable marker insulin resistance, AF. Thus, we aimed to investigate association between TyG and AF among hospitalized patients. Methods A retrospective observational study conducted in Daping Hospital, which included 356 patients from Department Cardiology. Clinical biochemical parameters...
Abnormal vascular smooth muscle cell (VSMC) proliferation is involved in the development of diseases. However, mechanisms by which insulin exerts this effect are not completely known. We hypothesize that microRNAs might be insulin-induced VSMC proliferation.VSMC was determined [H]-thymidine incorporation; were microRNA chips and real-time PCR; p21expression immunoblotting.In study, we found increased miR-208 expression. Overexpression basal insulin-mediated proliferation. Although a...
To investigate long noncoding RNA NONHSAT112178 (LncPPARδ) as a biomarker for coronary artery disease (CAD) in peripheral blood monocyte cells, RT-qPCR was performed to validate the microarray results, receiver operating characteristic curve applied study potential of LncPPARδ biomarker. Diagnostic models from alone or combination risk factors were constructed by Fisher criteria. The expression genes neighboring gene examined with THP-1 cell line treated siRNA. Using diagnostic model...
Abstract Aims Exposure to maternal diabetes is associated with increased prevalence of hypertension in the offspring. The mechanisms underlying prenatal programming remain unclear. Because endoplasmic reticulum (ER) stress plays a key role vascular endothelial dysfunction hypertension, we investigated whether aberrant ER causes and high blood pressure offspring dams diabetes. Methods results Pregnant Sprague-Dawley rats were intraperitoneally injected streptozotocin (35 mg/kg) or citrate...
Abnormalities in D1 dopamine receptor function the kidney are present some types of human essential and rodent genetic hypertension. We hypothesize that increased activity G protein-coupled kinase type 4 (GRK4) causes impaired renal measured GRK4 serine-phosphorylated receptors determined effect decreasing protein by chronic cortical interstitial infusion (4 weeks) antisense oligodeoxynucleotides (As-Odns) conscious- uninephrectomized spontaneously hypertensive rats (SHRs) their normotensive...
Dopamine is an important modulator of blood pressure, in part, by regulating vascular resistance. To test the hypothesis that D(1) and D(3) receptors interact smooth muscle cells, we studied A10 a rat aortic cell line, mesenteric arteries express both dopamine receptor subtypes. Fenoldopam, D(1)-like agonist, increased protein time-dependent concentration-dependent manner cells. The effect fenoldopam was specific because antagonist, SCH23390 (10(-7) M/24 h), completely blocked stimulatory h)...