A5040284746- Parathyroid Disorders and Treatments
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Dialysis and Renal Disease Management
- Chemical Synthesis and Analysis
- Muscle Physiology and Disorders
- Renal and related cancers
- Birth, Development, and Health
- Tissue Engineering and Regenerative Medicine
- Vitamin D Research Studies
- Ubiquitin and proteasome pathways
- Mesenchymal stem cell research
- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Bone health and treatments
- Bone health and osteoporosis research
- Histone Deacetylase Inhibitors Research
- Antimicrobial Peptides and Activities
- Liver physiology and pathology
- Advanced MRI Techniques and Applications
- Body Composition Measurement Techniques
- Multiple Myeloma Research and Treatments
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Renin-Angiotensin System Studies
National Institute of Health Sciences
2020-2025
Yokohama City University
2022-2025
Akita Prefectural University
2019
Japan Broadcasting Corporation (Japan)
2019
Shionogi (Japan)
2015-2017
Futaba (Japan)
2015
Keio University Hospital
2015
Osaka University
2003-2014
Osaka National Hospital
2012-2013
Hokkaido University
2012
Accumulation of adipocytes and collagen type-I-producing cells (fibrosis) is observed in muscular dystrophies. The origin these had been largely unknown, but recently we identified mesenchymal progenitors positive for platelet-derived growth factor receptor alpha (PDGFRα) as the skeletal muscle. However, muscle fibrosis remains unknown. In this study, clonal analyses show that PDGFRα+ also differentiate into cells. fact, accumulated fibrotic areas diaphragm mdx mouse, a model Duchenne...
ABSTRACT. The renal glomerulus, whose cellular components are developmentally derived from the mesenchyme, plays a pivotal role in filtratating plasma. Irretrievable changes of glomerular responsible for initiation and progression impaired function. Recently, it has been shown that functional stem cells exist bone marrow adult bodies they can reconstitute damaged tissues mesenchymal origin. To examine whether provides to glomeruli, transgenic rats carrying enhanced green fluorescence protein...
Objective— Obesity is recognized increasingly as a major risk factor for kidney disease. We reported previously that plasma adiponectin levels were decreased in obesity, and had defensive properties against type 2 diabetes hypertension. In this study, we investigated the role of disease subtotal nephrectomized mouse model. Methods Results— Subtotal (5/6) nephrectomy was performed adiponectin-knockout (APN-KO) wild-type (WT) mice. The procedure resulted significant accumulation glomeruli...
Satellite cells, which are skeletal muscle stem divide to provide new myonuclei growing fibers during postnatal development, and then maintained in an undifferentiated quiescent state adult muscle. This is considered be essential for the maintenance of satellite but their molecular regulation unknown. We show that Hesr1 (Hey1) Hesr3 (Heyl) (which known Notch target genes) expressed simultaneously only cells. In single-knockout mice, no obvious abnormalities cells or regenerative potentials...
ABSTRACT. Renal proximal tubular cells activated by reabsorption of protein are thought to play significant roles in the progression kidney diseases. It was hypothesized that signal transducer and activator transcription (STAT) proteins may be proteinuria cells. To test this hypothesis, murine were treated with albumin (30 mg/ml medium) for various lengths time. The results showed could activate Stat1 Stat5 within 15 min activation STATs mediated mostly Jak2 required no synthesis. In...
ABSTRACT. The foot processes forming the slit diaphragm are disrupted in diseases associated with proteinuria. Although they often repairable, regulators for repairing process remain unknown. By extrapolating from fact that vitamin A is essential nephrogenesis, this study examined whether or not injured podocytes middle of require retinaldehyde dehydrogenase type 2 (RALDH2), one key enzymes to produce all-trans-retinoic acid (ATRA). RALDH2 was dramatically upregulated puromycin...
The renoprotective potential of the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonist pioglitazone was explored in an immune model progressive nephropathy, passive Heymann nephritis (PHN), compared with that angiotensin II receptor antagonist, taken as standard therapy for renoprotection. PHN rats received orally vehicle, (10 mg/kg twice daily), or candesartan (1 daily) from months 2 to 8. Pioglitazone reduced proteinuria effectively and limited renal functional...
Recent studies have demonstrated that podocyte injury, which results in proteinuria, leads to tubulointerstitial fibrosis. Although some revealed vitamin D administration protects renal structure and function mesangial cell proliferative and/or excessive matrix productive models, the effects of on injury remained uncertain.In this study, we examined whether active (calcitriol) or its analogue, 22-oxacalcitriol (maxacalcitol), is preventative using puromycin aminonucleoside nephrosis model...
A group of muscular dystrophies, dystroglycanopathy is caused by abnormalities in post-translational modifications dystroglycan (DG). To understand better the pathophysiological roles DG modification and to establish effective clinical treatment for dystroglycanopathy, we here generated two distinct conditional knock-out (cKO) mice fukutin, first gene identified Fukuyama congenital dystrophy. The model—myofiber-selective fukutin-cKO [muscle creatine kinase (MCK)-fukutin-cKO] mice—showed mild...
Targeted protein degradation by proteolysis-targeting chimera (PROTAC) is one of the exciting modalities for drug discovery and biological discovery. It important to select an appropriate linker, E3 ligase ligand, a target ligand in development; however, it necessary synthesize large number PROTACs through trial error. Herein, using docking simulation ternary complex hematopoietic prostaglandin D synthase (H-PGDS) degrader, H-PGDS, cereblon, we have succeeded developing PROTAC(H-PGDS)-7 (6),...
Antimicrobial peptides (AMPs) are promising therapeutic agents against bacteria. We have previously reported an amphipathic AMP Stripe composed of cationic L-Lys and hydrophobic L-Leu/L-Ala residues, exhibited potent antimicrobial activity Gram-positive Gram-negative Gramicidin A (GA), repeating sequences L- D-amino acids, has a unique β
Muscle satellite cells are indispensable for muscle regeneration, but the functional diversity of their daughter is unknown. Here, we show that many Pax7+MyoD− locate both beneath and outside basal lamina during myofiber maturation. A large majority these not self-renewed cells, have different potentials proliferation differentiation from Pax7+MyoD+ myoblasts (classical cells), specifically marked by expression doublecortin (Dcx) gene. Transplantation lineage-tracing experiments demonstrated...
Although hematopoietic prostaglandin D synthase (H-PGDS) is an attractive target for treatment of a variety diseases, including allergic diseases and Duchenne muscular dystrophy, no H-PGDS inhibitors have yet been approved these diseases. Therefore, the development novel agents having other modes action to modulate activity required. In this study, chimeric small molecule that degrades via ubiquitin-proteasome system,
Mid-sized peptide therapeutics have gained significant attention for their potential to overcome the limitations of small molecules and biologics. However, clinical application is often hindered by poor stability low cellular permeability. In this study, we designed a palindromic foldamer composed L-leucine L-arginine residues investigate its structural functional properties. CD spectroscopy confirmed that adopts stable α-helical conformation, even under denaturing conditions. Cellular...