A5107925174- Genomics and Phylogenetic Studies
- Respiratory Support and Mechanisms
- Inflammation biomarkers and pathways
- Machine Learning in Bioinformatics
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- S100 Proteins and Annexins
- Single-cell and spatial transcriptomics
- Metabolism and Genetic Disorders
- Fractal and DNA sequence analysis
- Ferroptosis and cancer prognosis
- SARS-CoV-2 and COVID-19 Research
- Antibiotic Resistance in Bacteria
- Respiratory viral infections research
- Antifungal resistance and susceptibility
- Sepsis Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- interferon and immune responses
- RNA Research and Splicing
- Bacterial Genetics and Biotechnology
- Public Health and Nutrition
- Cardiac Arrest and Resuscitation
- Microbial Community Ecology and Physiology
- Virus-based gene therapy research
- Metabolomics and Mass Spectrometry Studies
Institute of Animal Sciences
2024-2025
Chinese Academy of Agricultural Sciences
2015-2025
Capital Normal University
2025
First Affiliated Hospital of Guangzhou Medical University
2014-2024
State Key Laboratory of Respiratory Disease
2010-2024
Guangzhou Medical University
2014-2024
Mianyang Central Hospital
2024
University of Electronic Science and Technology of China
2024
Eighth Affiliated Hospital of Sun Yat-sen University
2024
Sun Yat-sen University
2024
Corona virus disease 2019 (COVID-19) patients with severe immune abnormalities are at risk of cytokine release syndrome (CRS). The definition, prevention, and treatment symptoms CRS in critically ill COVID-19 important problems. We report a single-center case series 11 acute respiratory distress from First Affiliated Hospital Guangzhou Medical University China 26 January 2020 to 18 February 2020. termination date follow-up was 19 Eight were determined have characteristics CRS, including...
Abstract Background COVID-19 (coronavirus disease 2019) has caused a major epidemic worldwide; however, much is yet to be known about the epidemiology and evolution of virus partly due scarcity full-length SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) genomes reported. One reason that challenges underneath sequencing directly from clinical samples have not been completely tackled, i.e., with low viral load often results in insufficient reads for analyses. Methods We applied...
Haloferax mediterranei, an extremely halophilic archaeon, has shown promise for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated cheap carbon sources. Here we report the complete genome (3,904,707 bp) H. mediterranei CGMCC 1.2087, consisting one chromosome and three megaplasmids.
Abstract Background A significant proportion of septic patients with acute lung injury (ALI) are recognized late due to the absence an efficient diagnostic test, leading postponed treatments and consequently higher mortality. Identifying biomarkers may improve screening identify at high risk ALI earlier provide potential effective therapeutic drugs. Machine learning represents a powerful approach for making sense complex gene expression data find robust biomarkers. Methods The datasets were...
Genomic islands (GIs) that are associated with microbial adaptations and carry sequence patterns different from of the host sporadically distributed among closely related species. This bias can dominate signal interest in GI detection. However, variations still exist segments host, although no uniform standard exists regarding best methods discriminating GIs rest genome terms compositional bias. In present work, we proposed a robust software, MTGIpick, which used regions pattern showing...
Abstract Background Investigating cell fate decision and subpopulation specification in the context of neural lineage is fundamental to understanding neurogenesis neurodegenerative diseases. The differentiation process neural-tube-like rosettes vitro representative tube structures, which are composed radially organized, columnar epithelial cells give rise functional cells. However, underlying regulatory network commitment during early remains elusive. Results In this study, we investigated...
Understanding the mechanism underlying thermostabilization in naturally stable enzymes and enhancing thermostability of unstable are crucial aspects enzyme engineering. Despite development various engineering methods, there remains substantial scope for improvement. In this study, a novel concept termed as "short board" theory is proposed, which conceptualizes proteins barrels with each component representing jagged board. Notably, optimizing modifications to shortest board yields optimal...
Acute respiratory distress syndrome (ARDS) remains a high morbidity and mortality disease entity in critically ill patients, despite decades of numerous investigations into its pathogenesis. To obtain global protein expression changes acute lung injury (ALI) tissues, we employed high-throughput proteomics method to identify key components which may be involved the pathogenesis ALI. In present study, analyzed tissue proteomes Pseudomonas aeruginosa -induced ALI rats identified eighteen...
Background Immunosuppression is common even in the early stage of severe sepsis. Interleukin-10 (IL-10) secretion and lymphocyte exhaustion are main features sepsis-induced immunosuppression. However, relationship between IL-10 still unclear. We investigated if IL-10/lymphocyte ratio (IL10LCR) were associated with mortality septic patients. Methods Adult patients sepsis admitted to ICU First Affiliated Hospital Guangzhou Medical University identified from October 2012 August 2013. Within 24...
Advances in DNA sequencing technologies have transformed our capacity to perform life science research, decipher the dynamics of complex soil microbial communities and exploit them for plant disease management. However, is a conglomerate, which makes functional metagenomics studies very challenging.Metagenomes were assembled by long-read (PacBio, PB), short-read (Illumina, IL), mixture PB IL (PI) samples compared. Ortholog analyses annotation revealed that PI approach significantly increased...
Abstract Background While multiple replication origins have been observed in archaea, considerably less is known about their evolutionary processes. Here, we performed a comparative analysis of the predicted (proved part) orc/cdc6 -associated 15 completely sequenced haloarchaeal genomes to investigate diversity and evolution halophilic Archaea. Results Multiple were all analyzed following identification putative ORBs (origin recognition boxes) that are associated with genes. Five these...
Abstract The use of multiple origins for chromosome replication has been demonstrated in archaea. Similar to the dormant eukaryotes, some potential archaea appear be inactive during genome replication. We have comprehensively explored origin utilization Haloferax mediterranei . Here we report three active chromosomal by genome-wide profiling, and demonstrate that when these are deleted, a becomes activated. Notably, this cannot further deleted other already absent vice versa Interestingly,...
Cytochrome P450 enzymes (P450s) are promising candidates for the biosynthesis of 25-hydroxyvitamin D3 (25(OH)VD3). However, their industrial application is limited by challenges, such as low stability, inefficient catalysis, and uncoupling reactions. The construction self-sufficient P450s offers a strategic solution to these limitations, but requires linker optimization regulate interdomain conformational dynamics. In this study, we integrated whole-cell biocatalyst screening with systematic...
Imipenem-resistant Acinetobacter baumannii (IRAB) is an important cause of hospital-acquired infection. We aimed to describe outbreak IRAB infection and investigate its possible source in intensive care unit.An environmental investigation was undertaken. Antimicrobial susceptibility testing performed by microdilution. These isolates were genotyped use repetitive extragenic palindromic polymerase chain reaction (rep-PCR; DiversiLab). The study included 11 patients infected with 14 control...
Extracorporeal membrane oxygenation (ECMO) is increasingly being applied as life support for acute respiratory distress syndrome (ARDS) patients. However, the outcomes of this procedure have not yet been characterized in severe ARDS The aim study was to evaluate patients supported with ECMO and identify potential predictors mortality these A total 38 (aged 51.39±13.27 years, 32 males) who were treated specialized medical intensive care unit Guangzhou Institute Respiratory Diseases from July...
ABSTRACT COVID-19 has caused a major epidemic worldwide, however, much is yet to be known about the epidemiology and evolution of virus. One reason that challenges underneath sequencing HCoV-19 directly from clinical samples have not been completely tackled. Here we illustrate application amplicon hybrid capture (capture)-based sequencing, as well ultra-high-throughput metatranscriptomic (meta) in retrieving complete genomes, inter-individual intra-individual variations covering range sample...
Wnt/β-Catenin pathway plays an important role in the occurrence and progression of malignant tumors, especially PD-L1 -mediated tumor immune evasion. However, TCF/LEF , member Wnt/β-catenin pathway, immunosuppressive microenvironment lung adenocarcinoma (LUAD) remains unknown. LUAD tissue-coding RNA expression data from The Cancer Genome Atlas TIMER databases were used to analyze transcription factors their correlation with various cell infiltration. Immunohistochemistry immunofluorescence...