A5047865987- Infection Control and Ventilation
- Poxvirus research and outbreaks
- Bacillus and Francisella bacterial research
- Herpesvirus Infections and Treatments
- COVID-19 and healthcare impacts
- SARS-CoV-2 detection and testing
- Infection Control in Healthcare
- COVID-19 epidemiological studies
- SARS-CoV-2 and COVID-19 Research
- Healthcare and Environmental Waste Management
- Inhalation and Respiratory Drug Delivery
- Particle Dynamics in Fluid Flows
- Rabies epidemiology and control
- COVID-19 Pandemic Impacts
- Recycling and Waste Management Techniques
UK Health Security Agency
2022-2024
Public Health England
2020-2021
The transmission of SARS-CoV-2 is likely to occur through a number routes, including contact with contaminated surfaces. Many studies have used reverse transcription-PCR (RT-PCR) analysis detect RNA on surfaces, but seldom has viable virus been detected. This paper investigates the viability over time dried onto range materials and compares copies recovered whether concentration dependent. Viable persisted for longest surgical mask material stainless steel, 99.9% reduction in by 122 114 h,...
The survival of newer variants SARS-CoV-2 on a representative surface has been compared to the established UK circulating isolate determine whether enhanced environmental stability could play part in their increased transmissibility. Stainless steel coupons were inoculated with liquid cultures three variants, recovered over seven days and processed for recoverable viable virus using plaque assay. After drying, there was no significant difference inactivation rates between indicating that is...
Little is understood about the impact of nebulisation on viability SARS-CoV-2. In this study, a range nebulisers with differing methods aerosol generation were evaluated to determine SARS-CoV-2 following aerosolization. The particle size distribution was assessed using an sizer (APS) and determined after collection into liquid media All-Glass Impingers (AGI). Viable particles further characterised Collison 6-jet nebuliser in conjunction novel sample techniques Andersen size-fractioning...
Abstract Background An unprecedented outbreak of monkeypox virus (MPXV) infections in non-endemic countries has been recognised since 12 May 2022. More than 6000 cases have identified globally with more 1500 the UK by July Transmission MPXV is believed to be predominantly through direct contact lesions or infected body fluids, possible involvement fomites and large respiratory droplets. Importantly, a case healthcare worker 2018 was suspected due exposure while changing bedding. Methods We...
An outbreak of SARS-CoV-2 (1 March to 10 May 2021) with an attack rate 26.5% among approximately 1150 workers at a storage and distribution centre in England prompted multidisciplinary investigation (5 6 August 2021), the aim better understanding worker- workplace-related risk factors for viral transmission warehousing sector. Overall, environmental (e.g., ventilation, humidity temperature) were assessed be appropriate facility. Nevertheless, 39 (51.3%) surface samples from across site...
Most studies on surface persistence of SARS-CoV-2 have been conducted at temperatures between 20°C and 30°C. There is limited data the survival low temperatures. In this study, stability Alpha Delta variants stainless steel was investigated two (4°C 24°C). The results show that both decayed more rapidly 24°C compared with 4°C. At 24°C, showed reductions 0.33 log
To utilize environmental surface sampling to evaluate areas of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contamination within workplaces identify trends and improve local disease 2019 (COVID-19) control measures.Surface was undertaken at 12 that experienced a cluster COVID-19 cases in the workforce between March 2021 2022. A total 7.4% (61/829) samples collected were positive for SARS-CoV-2 RNA by quantitative PCR (qPCR) with only 1.8% (15/829) identified crossing...
The public order and safety (POS) sector remains susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreaks, as workplace attendance is typically compulsory close physical contact often needed. Here, we report on a SARS-CoV-2 outbreak with an attack rate of 39% (9/23), which occurred between 19 29 June 2021 among cohort new POS recruits participating in mandatory 18-week training programme England.
ABSTRACT Aims To utilise environmental surface sampling to evaluate areas of SARS-CoV-2 contamination within workplaces identify trends and improve local COVID-control measures. Methods Results Surface was undertaken at 12 that experienced a cluster COVID-19 cases in the workforce between March 2021 2022. 7.4% (61/829) samples collected were positive for RNA by qPCR with only 1.8% (15/829) identified crossing threshold (Ct) values below 35.0. No sample returned whole genome sequence...
Surfaces contaminated with infectious SARS-CoV-2 particles have the potential to cause human infection and any increase in surface survivability of a variant may its prevalence over other variants. This study investigated whether there were differences persistence between Delta Omicron variants leading Omicron's dominance globally. Stainless steel coupons inoculated suspensions either or exposed typical environmental conditions within containment level 3 laboratory. Coupons recovered at...
Abstract Understanding how Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is spread within the hospital setting essential if staff are to be adequately protected, effective infection control measures implemented and nosocomial transmission prevented. The presence of SARS-CoV-2 in air on environmental surfaces around hospitalised patients, with without respiratory symptoms, was investigated. Environmental sampling carried out eight hospitals England during first wave COVID-19...
Abstract The transmission of SARS-CoV-2 is likely to occur through a number routes, including contact with contaminated surfaces. Many studies have used RT-PCR analysis detect RNA on surfaces but seldom has viable virus been detected. This paper investigates the viability over time dried onto range materials and compares copies recovered, whether concentration dependant. Viable persisted for longest surgical mask material stainless steel 99.9% reduction in by 124 113 hours respectively....
The transport of infectious substances is a highly regulated process. European Agreement for International Carriage Dangerous Goods by Road instructs that specimens must be transported in triple packaging system containing leakproof receptacles. Despite regulatory efforts, leakages occur. Current evidence Parafilm usage lacking. This study evaluates the use preventing leakage from commonly used primary receptacles during ground transport. Two test methods, manual inversion and rocker, were...
Abstract In May 2022, an office worker attended their place of work while experiencing prodromal symptoms monkeypox infection. Environmental sampling performed four days later identified only low levels virus DNA contamination the worker’s desk, and no elsewhere within office. Replication-competent was not identified.
Environmental sampling played an important role in evaluating levels of environmental contamination present hospitals and outpatient settings during the mpox 2022 outbreak This allowed validation infection prevention control (IPC) measures identification potential routes transmission when caring for infected patients. Investigations typically focussed on high-risk settings, using quantitative polymerase chain reaction (qPCR) to identify presence virus (MPXV) DNA ( 1 Marimuthu K. Wong J.C.C....
Summary The survival of newer variants SARS-CoV-2 on a representative surface has been compared to the established UK circulating isolate determine whether enhanced environmental stability could play part in their increased transmissibility. Stainless-steel coupons were inoculated with liquid cultures three variants, recovered over seven days and processed for recoverable viable virus using plaque assay. After drying, there was no significant difference inactivation rates between variants....