A5100745160- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Diabetes and associated disorders
- COVID-19 Clinical Research Studies
- Circular RNAs in diseases
- Monoclonal and Polyclonal Antibodies Research
- Rheumatoid Arthritis Research and Therapies
- COVID-19 and Mental Health
- Diabetes Management and Research
- Long-Term Effects of COVID-19
- COVID-19 epidemiological studies
- Liver Disease Diagnosis and Treatment
- Tuberculosis Research and Epidemiology
- Immunotherapy and Immune Responses
- Mycobacterium research and diagnosis
- Radio Astronomy Observations and Technology
- Dermatological and COVID-19 studies
- Simulation-Based Education in Healthcare
- Healthcare professionals’ stress and burnout
- Cancer, Stress, Anesthesia, and Immune Response
- Cancer Mechanisms and Therapy
- CAR-T cell therapy research
- Phytochemicals and Antioxidant Activities
- Nursing education and management
- Cancer-related molecular mechanisms research
Laboratoire de Physique Nucléaire et de Hautes Énergies
2024
Linyi People's Hospital
2019-2021
Affiliated Hospital of Jining Medical University
2012-2021
Jining Medical University
2012-2014
The present study was targeted at investigating the effects of long intergenic non-protein coding RNA 1094 on breast cancer (BC) cell proliferation, apoptosis, and cycle its related mechanism. In this study, Western blot quantitative real-time polymerase chain reaction (qRT-PCR) were conducted to detect expressions LINC01094, microRNA (miRNA, miR)-340-5p, E2F transcription factor 3 (E2F3) in BC tissues cells. With transfection, LINC01094 miR-340-5p selectively up-regulated or down-regulated...
Background: Cervical cancer (CC) is one of the most common cancers among women worldwide. Circular RNAs (circRNAs) are recently identified as important gene regulators with critical roles in biology. In this study, we explored effects circ_0000388 on malignant phenotypes CC cells and its mechanism. Materials Methods: Circ_0000388 expression miR-337-3p tissue samples were measured using quantitative real time polymerase chain reaction. CCK-8 was adopted to assess effect cell line...
Background: Accumulating evidence has revealed that an increasing number of microRNAs (miRNAs) are dysregulated in papillary thyroid cancer (PTC) and their dysregulation plays important role PTC onset progression. Reportedly, miRNA-564 (miR-564) is downregulated several types human cancer. However, its expression profile specific functions remain unclear to date. Methods: In this study, we used reverse transcription-quantitative polymerase chain reaction detect miR-564 tissues cell lines....
The purpose of the article is to compare whole blood interferon-γ release assay (IGRA) with traditional methods for detecting Mycobacterium tuberculosis (MTB) infection in children. Fifteen childhood patients and 15 healthy children were recruited. Sputa samples venous collected, according different procedures, IGRA, sputum smear, colloidal gold (CGA), fluorescence quantitation polymerase chain reaction (FQ-PCR), skin test (TST) were, respectively, performed. Thirty vaccinated Bacillus...
To date, the complete mechanism of rheumatoid arthritis (RA) remain unclear, T cells have been proposed to play an important role in disease initiation and progression. Presently, some researchers reported that there were skewed TCR Vβ different samples experimental animals or RA patients, such as peripheral blood, joints synovial fluid, however, most results not coincident even conflict with each other. In this article, real-time fluorescence quantitative PCR DNA melting curving technique,...
To compare the sequences and crystal structures of variable region beta chain 7 (Vβ7) T cell receptor (TCR) two patients with type 1 diabetes mellitus (T1DM).The skewness TCR Vβ7 T1DM were detected real-time florescence quantitative polymerase reaction (FQ-PCR) deoxyribonucleic acid (DNA) melting curve analysis technique followed by being sequenced, them simulated according to CPH models 2.0 Server, IMGT database, RasMol 2 software.The whole patient-1 "CASRTAGQYEQYFGPGTR", that patient-2...
<b>Aim:</b> To detect the skewness of TCR Vα and Vβ patients with type 1 diabetes (T1D). <b>Methods:</b> The heparinized venous blood was collected from ten T1D. peripheral lymphocytes (PBL) were isolated used to extract mRNA. Reverse amplyfication performed for cDNA synthesis. detected real-time florescence quantitative polymerase chain reaction (RQ-PCR) analyzed by DNA melting-curve analysis technique, respectively. <b>Results:</b> Among genes, frequency rate (SFR) Vα22 30%; both Vα5 Vα24...
Objective. The objective of this study was to prepare, characterize, and determine immunological activities specific transfer factor (STF) human sperm antigen (HSA) for the preparation antisperm contraceptive vaccine that can be used as an immunocontraceptive. Methods. HSA-STF prepared using spleens rabbits vaccinated with HSA. were examined by lymphocyte proliferation test (LPT), leukocyte adhesion inhibition (LAIT), determining concentrations IL-4, γ -IFN, IL-21. a helveolous substance,...
Green tea (GT) is one of the most popular beverages in world, which contains numerous polyphenols known as catechins. Because high rate GT consumption worldwide populations, even small effects on an individual basis could have a large public health impact. Therefore, more and researches focus for human health. To date, identified important modifier diabetes, lipid metabolism, some haematological indices. Based previous reports, this study tries to investigate green partial serological...
The objective of the study was to explore methods for preparing transfer factor specific Staphylococcus aureus (SA-STF) in vitro. Under optimum conditions, spleen cells rabbits were immunized with SA vitro prepare SA-STF, and immune activities identified phagocytosis sterilization, skin delayed-type hypersensitivity, protection tests. concentration polypeptide 2.26 ± 0.27 mg/mL, ribose 0.684 0.094 mg/mL. sterilization rates STF group 70.9 12.4% 62.1 12.2%, respectively, compared non-specific...
Background: Severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) is still a severe threaft for human health currently, and the researches about it focus topic worldwide.
 Aim of study: In this study, we will collect some laboratory results patients with disease (COVID-19) to assess function liver, heart, kidney even pancreas. 
 Subjects Methods: Laboratory COVID-19 are collected. The biochemical indices classified used according kidney; meantime, blood glucose also observed...
<b>Objective: </b> To analyze the laboratory results of a Sjögrenâs syndrome combining autoimmune hepatitis (SS-AIH) with double negative SSA and SSB (SSA<SUP>-</SUP>SSB<SUP>-</SUP>). <b>Methods:</b><b> </b>10 ml venous blood was collected from patients, routine, biochemical immunological indices were respectively detected. <b>Results:</b> The values WBC, neutrophiles, ESR, ALT, AST, CRP, IgA IgG beyond corresponding reference ranges, respectively. antinuclear antibody pattern nuclear...
In this paper, we reanalyzed the data of ten reports related to variable region beta chain (Vβ) T cell receptors (TCR) patients with leukemia, and attempted further find their respective characters from a different point view. results, found that there were predominant usages TCR Vβ gene families. According usage frequencies (PUFs) Vβ, histograms drawn analyzed. The columns heights represented PUFs combination looked like key, every column was as same kit key. kits in leukemia. our opinions,...
The clinical education of nursing trainees should be an important task for the teaching hospital. However, this work is always ignored to some extent. In study, continuous quality improvement (CQI) approach was applied in trainees. result indicated that CQI could raise training trainees, including levels professionalism, communicative competence, theory and operation. Conclusion: Based on present results, we suggested used practice during period nurses.
<b>Obj</b><b>ective</b><b>: </b>To explore a fast and sensitive method for screening T cell receptor (TCR) biases through comparison of silver staining polyacrylamide gel electrophoresis ethidium bromide agarose electrophoresis. <b>M</b><b>ethods: </b>The lymphocytes were isolated from peripheral blood which collected ten patients with T1DM; the total RNA was extracted followed by transformed to cDNA, then carried out on simultaneously, finally stained dying respectively.<b> Results:...
T follicular helper (Tfh) cell is a new subpopulation of CD4+ family, whose differentiation affected by Bcl-6, Blimp-1, STAT3, STAT5 and so on, it could affect or decide the development other subsets cells. The important function Tfh to help B mediate humoral immunity, many researches have proved that cells participate in autoimmune disease, immunodeficient tumor infectious diseases. DOI: http://dx.doi.org/10.3329/jemc.v2i2.12843 J Enam Med Col 2012; 2(2): 85-91
Introduction. Chronic hepatitis B (CHB) is still a public health problem and its mechanism remains unclear. In this study, we detect the skewness of T cell receptor beta chain variable gene (TCR Vβ) in peripheral blood lymphocytes (PBL) liver infiltrating (LIL) patients with CHB; hope to provide information for further research on pathogenic CHB. Material methods. Fifteen CHB, ten healthy volunteers three cysts were recruited as subjects. The usage TCR Vβ PBL LIL measured compared;...
Objective To explore the key influencing factors of public protective action from angle risk perception, cognition and authority trust so as to build decision model in city with corona virus disease (COVID-19). Methods From 2ed February 2020 6th 2020, we carried out cross-sectional investigation among 1 201 publics selected by convenience sampling Wen Juan Xing electronic questionnaire collection system. The tool included general information COVID-19 decision-making scale. SPSS 22.0...