A5066990458- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Mitochondrial Function and Pathology
- Air Quality and Health Impacts
- Neuroscience and Neuropharmacology Research
- Hemoglobinopathies and Related Disorders
- Cancer, Hypoxia, and Metabolism
- Air Quality Monitoring and Forecasting
- Neonatal Health and Biochemistry
- Ferroelectric and Piezoelectric Materials
- Pregnancy and preeclampsia studies
- Peroxisome Proliferator-Activated Receptors
- S100 Proteins and Annexins
- Ion Transport and Channel Regulation
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- Venomous Animal Envenomation and Studies
- Signaling Pathways in Disease
- Microwave Dielectric Ceramics Synthesis
- Ion Channels and Receptors
- Neuroscience of respiration and sleep
- Complement system in diseases
- Gyrotron and Vacuum Electronics Research
- Thermal Regulation in Medicine
- Immune Response and Inflammation
Duke University
2020-2024
Central South University
2009-2024
Xiangya Hospital Central South University
2009-2024
Shaanxi Normal University
2023-2024
Duke University Hospital
2020-2023
Duke Medical Center
2020-2023
Durham University
2023
University of South Florida
2018-2022
Shanxi Normal University
2021
Peking University
2016-2021
Human trophoblast organoids (TOs) are a three-dimensional ex vivo culture model that can be used to study various aspects of placental development, physiology and pathology. However, standard culturing TOs does not recapitulate the cellular orientation chorionic villi in given multi-nucleated syncytiotrophoblast (STB) develops largely within inner facing surfaces these (STBin). Here, we developed method under conditions vivo. We show STBin suspension with gentle agitation leads development...
Abstract. To control severe air pollution in China, comprehensive strategies have been implemented throughout the country recent years. evaluate effectiveness of these strategies, influence meteorological conditions on levels needs to be determined. Using intensive during Asia-Pacific Economic Cooperation Forum 2014 (APEC 2014) and 2015 China Victory Day Parade (Victory 2015) as examples, we estimated role reducing Beijing. Atmospheric particulate matter aerodynamic diameter ≤ 2.5 µm (PM2.5)...
Renal ischemia-reperfusion injury is an important contributor to the development of delayed graft function after transplantation, which associated with higher rejection rates and poorer long-term outcomes. One earliest impairments during ischemia Na + /K -ATPase (Na/K pump) dysfunction due insufficient ATP supply, resulting in subsequent cellular damage. Therefore, strategies that preserve or maintain Na/K pump may limit extent renal ischemia-reperfusion. Here, we applied a synchronization...
TMEM16F, a Ca 2+ -activated phospholipid scramblase (CaPLSase), is critical for placental trophoblast syncytialization, HIV infection, and SARS-CoV2-mediated however, how TMEM16F activated during cell fusion unclear. Here, using trophoblasts as model fusion, we demonstrate that influx through the permeable transient receptor potential vanilloid channel TRPV4 activation plays role in subsequent human fusion. GSK1016790A, specific agonist, robustly activates trophoblasts. We also show are...
TMEM16F (also known as ANO6), a Ca2+-activated lipid scramblase (CaPLSase) that dynamically disrupts asymmetry, plays crucial role in various physiological and pathological processes, such blood coagulation, neurodegeneration, cell-cell fusion viral infection. However, the mechanisms through which it regulates these processes remain largely elusive. Using endothelial cell-mediated angiogenesis model, here we report previously unknown intracellular signaling function of TMEM16F. We...
PIEZO1, a mechanosensor 1,2 in endothelial cells, plays critical role fetal vascular development during embryogenesis 3,4 . However, its expression and function placental trophoblasts remain unexplored. Here, we demonstrate that PIEZO1 is expressed villus trophoblasts, where it essential for trophoblast fusion development. Mice with trophoblast-specific knockout exhibit embryonic lethality without obvious defects. Instead, deficiency disrupts the formation of syncytiotrophoblast layer...
Abstract Background The mutations of KCNMA1 BK‐type K + channel have been identified in patients with various movement disorders. underlying pathophysiology and corresponding therapeutics are lacking. Objectives To report our clinical biophysical characterizations a novel de novo variant, as well an effective therapy for the patient's dystonia‐atonia spells. Methods Combination phenotypic characterization, therapy, characterization patient her mutation. Results had >100 spells per day...
Although heat shock protein 70 (Hsp70) has been shown to markedly inhibit H(2)O(2)-induced apoptosis in C2C12 cells, and nucleolin/C23 also implicated apoptosis, the relationship of these two molecules is still largely unknown. The aim current study was investigate potential involvement antiapoptotic mechanism Hsp70. We found that primary cultures neonatal rat cardiomyocytes underwent upon H(2)O(2) treatment, cells highly unstable had a half-life less than 4 h. However, transfection with...
ABSTRACT The calcium-activated TMEM16 proteins and the mechanosensitive/osmolarity-activated OSCA/TMEM63 belong to Transmembrane Channel/Scramblase (TCS) superfamily. Within superfamily, proteins, as well TMEM16A TMEM16B, are thought function solely ion channels. However, most members, including TMEM16F, maintain an additional scramblases, rapidly exchanging phospholipids between leaflets of membrane. Although recent studies have advanced our understanding TCS structure-function...
In this work, a traditional solid-state method was adopted to prepare dense Ba[Formula: see text]Ca[Formula: text][(Zr[Formula: text]Ti[Formula: text])[Formula: text]–(Nb[Formula: text]La[Formula: text]) x ]O 3 (BCZT–xNL) lead-free relaxation ferroelectrics with excellent energy storage performance (ESP). Remarkably, large breakdown field strength of BDS ([Formula: text][Formula: text]kV/cm) resulting from decreased grain size by double ions doping at the B-site achieved in BCZT–xNL...
The calcium-activated TMEM16 proteins and the mechanosensitive/osmolarity-activated OSCA/TMEM63 belong to Transmembrane Channel/Scramblase (TCS) superfamily. Within superfamily, proteins, as well TMEM16A TMEM16B, are thought function solely ion channels. However, most members, including TMEM16F, maintain an additional scramblases, rapidly exchanging phospholipids between leaflets of membrane. Although recent studies have advanced our understanding TCS structure–function relationships,...
The calcium-activated TMEM16 proteins and the mechanosensitive/osmolarity-activated OSCA/TMEM63 belong to Transmembrane Channel/Scramblase (TCS) superfamily. Within superfamily, proteins, as well TMEM16A TMEM16B, likely function solely ion channels. However, remaining members, including TMEM16F, maintain an additional scramblases, rapidly exchanging phospholipids between leaflets of membrane. Although recent studies have advanced our understanding TCS structure-function relationships,...
TMEM16F, a dual-function phospholipid scramblase and ion channel, is important in blood coagulation, skeleton development, HIV infection, cell fusion. Despite advances understanding its structure activation mechanism, how TMEM16F regulated by intracellular factors remains largely elusive. Here we report that lipid scrambling channel activities are strongly influenced pH (pHi). We found low pHi attenuates, whereas high potentiates, under physiological concentrations of Ca2+ ([Ca2+]i). further...
Abstract. Vehicle emissions are a major source of air pollution in urban areas and thus greatly impact quality the megacity Beijing. Various vehicle emission control policies have been implemented at great cost, but there is lack appropriate methods to evaluate effectiveness such policies. Here we developed wavelet transform method (WTM) during 2014 Asia-Pacific Economic Cooperation (APEC) summit, taking advantage high-time-resolution mobile measurements NO, NOx, BC, CO, SO2, O3 made around...
Ambient particulate matter (PM) is often used as a proxy of personal exposure in epidemiological studies PM-induced health effects, yet whether this biases the estimates effects still unknown. On basis panel study Beijing, China, we investigated dependence 24 h concentration to fine particles (PM2.5) and its carbonaceous components on corresponding ambient concentration. The associated changes inflammatory biomarkers with were further examined using linear mixed-effect models. At PM2.5...
TMEM16 Ca2+-activated phospholipid scramblases (CaPLSases) mediate rapid transmembrane flip-flop and as such play essential roles in various physiological pathological processes blood coagulation, skeletal development, viral infection, cell-cell fusion, ataxia. Pharmacological tools specifically targeting CaPLSases are urgently needed to understand these novel membrane transporters their contributions health disease. Tannic acid (TA) epigallocatechin gallate (EGCG) were recently reported...