A5000481953- Cardiac electrophysiology and arrhythmias
- Cardiac Arrest and Resuscitation
- HIV Research and Treatment
- Atrial Fibrillation Management and Outcomes
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Ultrasound in Clinical Applications
- Analytical Chemistry and Chromatography
- Mechanical Circulatory Support Devices
- Immune Cell Function and Interaction
- Education, Psychology, and Social Research
- Hemodynamic Monitoring and Therapy
- Cardiac Arrhythmias and Treatments
- vaccines and immunoinformatics approaches
- Pleural and Pulmonary Diseases
- Sepsis Diagnosis and Treatment
- Scientific Computing and Data Management
- Renal function and acid-base balance
- Herpesvirus Infections and Treatments
- Radiation Dose and Imaging
- Cardiovascular Health and Disease Prevention
- Environmental Monitoring and Data Management
- Remote Sensing and LiDAR Applications
- RNA and protein synthesis mechanisms
- Trauma, Hemostasis, Coagulopathy, Resuscitation
Charles University
2004-2024
General University Hospital in Prague
2017-2024
Ghent University Hospital
2024
Institute of Political Studies
2024
Czech Academy of Sciences, Institute of Biotechnology
2018-2023
Czech Academy of Sciences
2018-2023
University Hospital Kralovske Vinohrady
2023
Czech Academy of Sciences, Institute of Physiology
2023
National Institute of Public Health
2006-2016
Comenius University Bratislava
2013
Definitive guidelines for anticoagulation management during veno-venous extracorporeal membrane oxygenation (VV ECMO) are lacking, whereas bleeding complications continue to pose major challenges.
Abstract The linear theory of electromigration, including the first‐order nonlinear approximation, is generalized to systems with any equilibria fast enough be considered instantaneous in comparison timescale peak movement. For example, this practically applied electrokinetic chromatography (EKC) mode CZE. model enables calculation positions and shapes analyte system peaks without restricting number selectors, complexation stoichiometry, or simultaneous acid–base equilibria. latest version...
Abstract Thermodynamic acidity constants (acid or acid‐base dissociation constants, sometimes called also as ionization constants) and limiting ionic mobilities (both of them at defined temperature, usually 25°C) are the fundamental physicochemical characteristics a weak electrolyte, that is, acid base ampholyte. We introduce novel method for determining data electrolyte by nonlinear regression effective electrophoretic mobility versus buffer composition dependence when measured in set BGEs...
A detailed description of the dnatco.datmos.org web server implementing universal structural alphabet nucleic acids is presented. It capable processing any mmCIF- or PDB-formatted files containing DNA RNA molecules; these can either be uploaded by user supplied as wwPDB PDB-REDO database access code. The performs an assignment acid conformations and presents results for intuitive annotation, validation, modeling refinement acids.
Abstract Fourteen low molecular mass UV absorbing ampholytes containing 1 or 2 weakly acidic and basic functional groups that best satisfy Rilbe's requirement for being good carrier (Δp K a = p monoanion ‒ monocation < 2) were selected from large group of commercially readily available in computational study using two software packages (ChemSketch SPARC). Their electrophoretic mobilities measured 10 mM ionic strength BGEs covering the pH 12 range. Using our Debye‐Hückel Onsager‐Fuoss...
Multiple molecular targets have been identified to mediate membrane-delimited and nongenomic effects of natural synthetic steroids, but the influence steroid metabolism on neuroactive signaling is not well understood. To begin address this question, we set out identify major metabolites a neuroprotective 20-oxo-5β-pregnan-3α-yl l-glutamyl 1-ester (pregnanolone glutamate, PAG) characterize their GABAA NMDA receptors (GABARs, NMDARs) zebrafish behavior. Gas chromatography–mass spectrometry was...
Abstract The article proposes a solution for object classification using multiple views generated from 3D data rendering and convolutional neural networks. For presentation purposes easier verification of the solution, an application was developed to create objects, classify them selected CNN, evaluate performance CNN. evaluation is based on metrics characteristics described in article. Seven testing objects were used verify proposed solution; five CNNs tested each.
The echocardiography parameters may predict the maintenance of sinus rhythm after cardioversion a supraventricular arrhythmia (SVA).
Interleukin 17 (IL-17) and its cognate receptor A (IL-17RA) play a crucial role in Th17 cells-mediated pro-inflammatory pathway pathogenesis of several autoimmune disorders including psoriasis. IL-17 is mainly produced by activated Th-17 helper cells upon stimulation IL-23 and, via binding to receptors, mediates IL-17-driven cell signaling keratinocytes. Hyper-proliferation keratinocytes belongs major clinical manifestations To modulate IL-17-mediated inflammatory cascade, we generated...
Water plays an important role in stabilizing the structure of DNA and mediating its interactions. Here, hydration was analyzed terms dinucleotide fragments from ensemble 2727 nonredundant chains containing 41 853 dinucleotides 316 265 associated first-shell water molecules. The were classified into categories based on their 16 sequences previously determined structural classes known as nucleotide conformers (NtCs). construction hydrated building blocks allowed to be calculated probability...
Abstract A simple, sensitive, specific, and cost‐effective analytical methodology was developed for the analysis of human plasma samples spiked with imatinib by CZE on‐line UV detection in context Therapeutic Drug Monitoring. Several conditions such as ionic strength ( I ) pH BGE composed citric acid ε‐amino caproic were studied regards presence sodium chloride (NaCl) (1% m/v). Computer simulations (Simul software) used to confirm experimental results understand electrophoretic behavior...
Introduction Supraventricular arrhythmias contribute to haemodynamic compromise in septic shock. A retrospective study generated the hypothesis that propafenone could be more effective than amiodarone achieving and maintaining sinus rhythm (SR). Certain echocardiographic parameters may predict a successful cardioversion help decision on or rate control strategy. Methods analysis The trial includes shock patients with new-onset arrhythmia, but without severe impairment of left ventricular...
The development of an effective vaccine preventing HIV-1 infection is hindered by the enormous antigenic variability and unique biochemical immunological properties Env glycoprotein, most promising target for neutralizing antibody. Functional studies rare elite neutralizers led to discovery broadly antibodies.We employed a highly complex combinatorial protein library derived from 5 kDa albumin-binding domain scaffold, fused with support total 38 kDa, screen binders antibody VRC01 paratope....