A5027507423- Neuroscience of respiration and sleep
- Animal Genetics and Reproduction
- melanin and skin pigmentation
- Sleep and Wakefulness Research
- Developmental Biology and Gene Regulation
- Congenital gastrointestinal and neural anomalies
- Neurobiology and Insect Physiology Research
- Retinal Development and Disorders
- Connexins and lens biology
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Silk-based biomaterials and applications
- Neonatal Respiratory Health Research
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
- Gastrointestinal motility and disorders
- Congenital limb and hand anomalies
- dental development and anomalies
- Adipose Tissue and Metabolism
- Ubiquitin and proteasome pathways
- Nuclear Receptors and Signaling
- Animal Vocal Communication and Behavior
- Obstructive Sleep Apnea Research
- Cellular Mechanics and Interactions
- Neonatal and fetal brain pathology
Collège de France
1994-2024
Centre National de la Recherche Scientifique
1998-2024
Centre Interdisciplinaire de Recherche en Biologie
2021-2024
Inserm
2010-2024
Université Paris Sciences et Lettres
2022-2024
Institut des Neurosciences Paris-Saclay
2022
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2022
Institut Curie
2001-2010
Howard Hughes Medical Institute
1998
University of Tsukuba
1998
ABSTRACT Interstitial cells of Cajal (ICC) aroused much interest among neuroanatomists at the beginning century. These small cells, organized into networks, are intercalated between nerve fibers and muscle now considered by many authors to be responsible for pacemaker activity gut. Renewed in these arose recently when receptor tyrosine kinase, ckit, was shown associated with their functional activity. The embryonic origin interstitial has remained a controversial issue ever since discovery....
Genetic data in the mouse have shown that endothelin 3 (ET3) and its receptor B (ETRB) are essential for development of two neural crest (NC) derivatives, melanocytes enteric nervous system. We report here effects ET3 vitro on differentiation quail trunk NC cells (NCC) mass clonal cultures. Treatment with is highly mitogenic to undifferentiated NCC population, which leads expansion population melanocytic, a lesser extent, glial lineages. The effect these derivatives was confirmed by...
Disruptions of the genes encoding endothelin 3 (EDN3) and its receptor endothelin-B (EDNRB) in mouse result defects two neural crest (NC)-derived lineages, melanocytes, enteric nervous system. To assess mechanisms through which EDN3/EDNRB signaling pathway can selectively act on these NC derivatives, we have studied spatiotemporal expression pattern EDNRB gene avian embryo, a model development has been extensively studied. For this purpose, cloned quail homologue mammalian cDNA. transcripts...
Endothelin 3 (EDN 3) and the endothelin receptor B (EDNRB) are involved in development of neural crest particularly melanocytes enteric nervous system. We reported previously that avian EDNRB gene is expressed fold before cell migration later on all derivatives except, at any developmental stage, melanocytic lineage. However, quail melanoblasts proliferate response to EDN stimulation vitro . These observations prompted us search for another type (EDNR). report here cloning by reverse...
Prostaglandin D2 (PGD2) is one of the most potent endogenous sleep-promoting molecules. However, cellular and molecular mechanisms PGD2-induced activation neurons in ventrolateral preoptic nucleus (VLPO), major nonrapid eye movement (NREM)-sleep center, still remains unclear. We here show that PGD2 receptors (DP1) are not only expressed leptomeninges but also astrocytes from VLPO. further demonstrate, by performing real-time measurements extracellular adenosine using purine enzymatic...
Basic‐leucine zipper transcription factors of the Maf family are key regulators various developmental and differentiation processes. We previously reported that phosphorylation status MafA is a critical determinant its biological functions. Using Western blot mass spectrometry analysis, we now show phosphorylated by p38 MAP kinase identify three phosphoacceptor sites: threonine 113 57, evolutionarily conserved residues located in activating domain, serine 272. Mutation these severely...
Abstract During brain maturation, astrocytes establish complex morphologies unveiling intense structural plasticity. Connexin 30 (Cx30), a gap‐junction channel‐forming protein expressed postnatally, dynamically regulates during development astrocyte morphological properties by controlling ramification and extension of fine processes. However, the underlying mechanisms remain unexplored. Here, we found in vitro that Cx30 interacts with actin cytoskeleton inhibits its reorganization dynamics...
Abstract We describe here the expression of c ‐ kit and Steel ( Sl ) genes during development melanocytes in normally pigmented strains chick quail compared to unpigmented (White Leghorn) hyperpigmented (Silky Fowl) chickens. By using quail/chick chimera system, we found that neural crest cells, which migrate dorso‐laterally subectodermal mesenchyme give rise melanocytes, express as early E4, is about 2 days after they have left primordium. The gene expressed from E4 onward epidermis but not...
Dorsoventral pattern formation of the optic cup is essential for vertebrate eye morphogenesis and retinotectal topographic mapping. Dorsal ventral aspects are distinct at early stages development; cVax homeodomain protein expression confined to cup, whereas Tbx5 (T-box transcription factor) domain becomes restricted dorsal region. Misexpression or induces profound defects in morphology abnormal visual projections. In Pax6-/- mutant fails be expressed, Vax1 -2 abnormally present entire...
Astroglial release of molecules is thought to actively modulate neuronal activity, but the nature, pathway, and cellular targets these neuroactive are still unclear. Pannexin 1, expressed by neurons astrocytes, form nonselective large pore channels that mediate extracellular exchange molecules. The functional relevance has been mostly studied in brain tissues, without considering their specific role different cell types, or neurons. Thus, our knowledge astroglial pannexin 1 regulation its...
The pattern of feather buds in a tract is thought to result from the relative ratios between activator and inhibitor signals through lateral inhibition process.We analyse role Drm/Gremlin, BMPs antagonist expressed during formation, dermal precursor, dense dermis, interbud dermis posterior condensation.We have altered activity Drm embryonic chick skin using retroviral vectors expressing drm/ gremlin bmps.We show that expression endogenous drm under control feedback loop induced by BMP...
Dorsal root ganglia proceed from the coalescence of cell bodies sensory neurons, which have migrated dorsoventrally delaminating neural crest. They are composed different neuronal subtypes with specific functions, including nociception, thermal sensation, proprioception, and mechanosensation. In contrast to proprioceptors thermonociceptors, little is known about molecular mechanisms governing early commitment later differentiation into mechanosensitive neurons. This mainly due absence...
In the Silky Fowl (SF) breed of chicken, most internal organs are infiltrated with melanocytes. Previous studies have shown that this generalized mesodermal pigmentation is not due to a cell autonomous abnormality melanocytes but environmental factors able promote both homing pigment precursors in abnormal embryonic sites and their proliferation differentiation. To analyse mode these cues, we tested effect SF embryo extract (SFEE) on cultured quail neural crest cells as compared EE from...
While apneas are associated with multiple pathological and fatal conditions, the underlying molecular mechanisms remain elusive. We report that a mutated form of transcription factor Mafa (Mafa4A) prevents phosphorylation protein leads to an abnormally high incidence breath holding death in newborn Mafa4A/4A mutant mice. This apneic breathing is phenocopied by restricting mutation central GABAergic inhibitory neurons activation while reversed inhibiting transmission centrally. find activates...