A5004996148- Ocular Infections and Treatments
- Orthopedic Infections and Treatments
- Medical Device Sterilization and Disinfection
- Antibiotic Resistance in Bacteria
- Antibiotics Pharmacokinetics and Efficacy
- Surgical Sutures and Adhesives
- Ocular Surface and Contact Lens
- Essential Oils and Antimicrobial Activity
- Bacterial Identification and Susceptibility Testing
- Orthopaedic implants and arthroplasty
- Bee Products Chemical Analysis
- Nosocomial Infections in ICU
- Surgical site infection prevention
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Infection Control in Healthcare
- Antimicrobial agents and applications
- Neonatal and Maternal Infections
- Phytochemistry Medicinal Plant Applications
- Dental Research and COVID-19
- Diatoms and Algae Research
- Cardiac pacing and defibrillation studies
- Pharmaceutical and Antibiotic Environmental Impacts
- CAR-T cell therapy research
- Elder Abuse and Neglect
Faculdade de Ciências Médicas da Santa Casa de São Paulo
2011-2021
Irmandade da Santa Casa de Misericórdia de São Paulo
2009-2021
Czech Academy of Sciences, Institute of Microbiology
2019
Santa Casa Hospital
2019
Universidade de São Paulo
2015
Novo Mel (Brazil)
2011
ABSTRACT Previous studies have shown that sonication fluid cultures from removed orthopedic devices improved the microbiological diagnosis of implant-associated infections; however, few these investigations applied to fracture fixation evaluate its utility for osteosynthesis-associated infection (OAI). We compared conventional tissue 180 subjects with different sizes plates and screws ( n = 156), spinal implants 26), intramedullary nails 3), whom 125 55 had OAI noninfected osteosynthesis...
Abstract Gram-negative bacteria (GNB) are a major cause of neonatal sepsis in low- and middle-income countries (LMICs). Although the World Health Organization (WHO) reports that over 80% these deaths could be prevented through improved treatment, efficacy currently recommended first- second-line treatment regimens for this condition is increasingly affected by high rates drug resistance. Here we assess three well known antibiotics, fosfomycin, flomoxef amikacin, combination as potential...
To compare cultured microorganisms identified on endotracheal tubes biofilms through sonication technique with traditional tracheal aspirate collected at extubation of pediatric intensive care unit patients.Demographic and epidemiological data were analyzed to identify factors possibly related the microbiological profile two collection methods. Associations between categorical continuous variables using chi-square or Fisher's exact test, Student's t test. p-Value <0.05 considered...
Staphylococcus aureus (S. aureus) is one of the most frequent causes hospital acquired infections. With increase in multiple drug resistant strains, natural products such as propolis are a stratagem for new product discovery. The aims this study were: to determine vitro antimicrobial activity an ethanol extract propolis; define MIC50 and MIC90 (Minimal Inhibitory Concentration - MIC) against 210 strains S. aureus; characterize crude sample respective presence predetermined chemical markers....
The clinical utility of sonication as an adjunctive diagnostic tool for the microbial diagnosis cardiac implantable device-associated infections (CIDAIs) was investigated.The implants 83 subjects were investigated, 15 with a CIDAI and 68 without infection. Clinical data analyzed prospectively fluid cultures (83 patients, 100%) traditional (31 37.4%) performedGenerator pocket infection device-related endocarditis found in 13 (86.7%) four (26.7%) subjects, respectively. mean numbers previous...
Background In the absence of a gold standard criterion for diagnosing prosthetic joint infections (PJI), sonication removed implant may provide superior microbiological identification to synovial fluid and peri-implant tissue cultures. The aim this retrospective study was assess role culture compared cultures PJI, using different consensus international guidelines PJI definition. Methods Data 146 patients undergoing removal hip or knee arthroplasties between 2010 2018 were retrospectively...
Microbial identification of orthopedic implant-associated infections using sonication fluid (SF) submitted to a concentration step by membrane filtration (SMF) was compared with the standard centrifugation (SC) method. Among 33 retrieved infected implants, identified microorganisms in 26 (78.8%). The sensitivity SC higher than that SMF (78.8% versus 30.3%; P < 0.001).
To investigate the binding affinity, stability, and sterility of aflibercept ziv-aflibercept to vascular endothelial growth factor (Holash et al. in Proc Natl Acad Sci USA 99(17):11393–11398, 2002. https://doi.org/10.1073/pnas.172398299 ) after compounding storage for up 28 days at 4 °C − 8 °C. Tuberculin-type 1-mL syringes were prepared containing (40 mg/mL) (25 mg/mL). Samples stored 0, 14, evaluated affinity anti-VEGF VEGF stability using enzyme-linked immunosorbent assays. The evaluation...
To analyze the antimicrobial properties of silicon oil (Óleo de Silicone®, Ophthalmos, Brazil) on in vitro bacterial growth different microorganisms related to endophthalmitis.The following were analyzed: (1) Pseudomonas aeruginosa (ATCC 27583); (2) Escherichia coli 25922); (3) Staphylococcus aureus 25923); (4) epidermidis 12228); (5) Candida albicans 10231); (6) Klebsiella pneumoniae 13883); and (7) Streptococcus 49619). The plates incubated at 35 ± 2ºC its examined after 24 hours. An empty...
To describe the microbiological findings from bandage contact lenses in patients who work a hospital environment submitted to photorefractive keratectomy (PRK).This prospective comparative case series enrolled 43 eyes of 22 volunteers (28.05 ± 3.50 years). Fourteen individuals (n = 27) were health care professionals facilities or community physician's offices. Eight 16) do not environment. Photorefractive was performed using standard technique, and silicone hydrogel lens placed on cornea...
Hospital-acquired infections account for high mortality rates and hospital costs. We analyzed pediatric data from a tertiary teaching found that most of the cases occurred in intensive care unit had significant association with invasive devices. Bloodstream were main site infection, Gram-negative bacteria predominant etiology.
Abstract: Relevance: This study demonstrated the synergism of various natural preparations in combination with antibiotics, which is a matter high potential therapeutic interest due to de- velopment bacterial resistance these antibiotic drugs. very relevant because it demonstrates that extracts and their association antibiotics may be viable thera- peutic options against pathogens are responsible for incidence nosocomial community infections. Aims: The investigated antibacterial activity...
To evaluate the efficacy of disinfecting solutions in hydrophilic contact lenses (CL).Two multi-use denominated solution A (0.001% polyquaternium-1 and 0.0005% myristamidopropyl dimethylamine) B (0.0001% polyaminopropyl biguanide) were used. The tested infected with Pseudomonas aeruginosa (ATCC27583), Staphylococcus epidermidis (ATCC1226), Klebsiella pneumoniae (ATCC13883), aureus (ATCC25923) Candida albicans (ATCC 10231) decrease microorganisms growth after cleaned respective was verified....
Purpose: To analyze the antibacterial effects in vitro of ethyl-cyanoacrylate (EC) tissue adhesive different application volumes. Methods: Volumes 4, 6, 8, 10, 12, 14, 25, and 35 μ l EC were applied onto surface monolayer cultures Staphylococcus aureus (ATCC25924), Streptococcus pneumoniae (ATCC49619), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), Klebsiella (ATCC13883). The diameter each drop was measured, area circle (formed after its culture) calculated. inhibitory...
ABSTRACT Objective: assess the safety of steam sterilization assembled laparoscopic instruments with challenge contamination. Method: a laboratory experimental study, using as test samples trocars and graspers. Geobacillus stearothermophillus ATCC-7953 was used, microbial population 106UFC/Filter paper substrate, removed from biological indicator. Three them were introduced into each instrument at time assembly, sterilized pressurized saturated steam, 134oC for 5 minutes. After...
To compare the antimicrobial effect in vitro of a short-chain cyanoacrylate with long-chain (Dermabond, Ethicon, Johnson and Johnson, USA) against bacterial strains.The following strains were analysed: Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia Pseudomonas aeruginosa. For each microorganism, standardised sterile discs (6 mm) containing 10 µL ethyl-cyanoacrylate 2-octyl applied to plate. All plates received blank filter-paper disc no adhesive (control). incubated for 24...
Objetivos: Avaliar a eficácia de um procedimento operacional padrão para limpeza fresas intramedulares flexíveis, bem como o alcance da esterilidade, e evidenciara citotoxicidade sujidade residual uma fresa flexível utilizada na prática assistencial. Métodos: Fresas flexíveis foram pesadas antes do processamento,após contaminação desafio depois limpeza. Elas contaminadas com Soil Test™, suspensão Geobacillus stearothermophilus, concentração de106 UFC/mL, farinha osso bovino. Após...
From July 2009 to 2015, Staphylococcus aureus isolated from pediatric sterile sites were selected. Polymerase chain reaction was used detect mecA and lukS-PV/lukF-PV genes. The rate of methicillin-resistant 37.7%. Ten isolates had the genes, 2 which . Skin soft tissues infections significantly associated with positive isolates, P = 0.008.
To evaluate in vitro the antimicrobial influence of corneal storage media containing antibiotics using a closed chamber study model under closest simulated environment preservation process.Samples cornea streptomycin and gentamicin (Optisol-GS) were analyzed at different moments after its contamination with Staphylococcus aureus (ATCC25923). Samples 1, 2, 3, 4, 5, 6, 24, 48, 72 hours; 7 days; 14 days contamination. The samples new system that consists two coupled chambers. upper contained...