A5059983180- Genomics and Phylogenetic Studies
- Plant Ecology and Taxonomy Studies
- Genetic diversity and population structure
- CRISPR and Genetic Engineering
- Neuroblastoma Research and Treatments
- Plant and Fungal Species Descriptions
- Landslides and related hazards
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Cellular Mechanics and Interactions
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Hemoglobinopathies and Related Disorders
- Cancer, Hypoxia, and Metabolism
- Fire effects on ecosystems
- Advanced Fluorescence Microscopy Techniques
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Amino Acid Enzymes and Metabolism
- Plant Diversity and Evolution
Chinese Academy of Sciences
2014-2025
University of Chinese Academy of Sciences
2014-2025
Henan Agricultural University
2025
Beijing Normal University
2023-2025
Shandong University
2024-2025
Institute of Geology and Geophysics
2023-2025
Renji Hospital
2025
Shanghai Jiao Tong University
2025
Center for Life Sciences
2025
Cancer Institute of New South Wales
2024-2025
A novel variant of the SARS-CoV-2 virus carrying a point mutation in Spike protein (D614G) has recently emerged and rapidly surpassed others prevalence. This is linkage disequilibrium with an ORF1b (P314L), making it difficult to discern functional significance D614G from population genetics alone. Here, we perform site-directed mutagenesis on wild-type human-codon-optimized introduce variant. Using multiple human cell lines, including lung epithelial cells, found that lentiviral particles...
The family Brassicaceae encompasses diverse species, many of which have high scientific and economic importance. Early diversifications phylogenetic relationships between major lineages or clades remain unclear. Here we re-investigate phylogeny with complete plastomes from 51 species representing all four 5 6 (A, B, C, E F) as identified in earlier studies. Bayesian maximum likelihood analyses using a partitioned supermatrix 77 protein coding genes resulted nearly identical tree topologies...
The recent characterization of RNA-targeting CRISPR nucleases has enabled diverse transcriptome engineering and screening applications that depend crucially on prediction selection optimized guide RNAs (gRNAs). Previously, we developed a computational model to predict RfxCas13d gRNA activity for all human protein-coding genes. Here, extend this framework six organisms (human, mouse, zebrafish, fly, nematode, flowering plants) genes noncoding (ncRNAs) also four RNA virus families (severe...
Estrogen receptor α (ERα) plays a pivotal role in the proliferation, differentiation, and migration of breast cancer (BC) cells, aromatase (ARO) is crucial enzyme estrogen synthesis. Hence, it necessary to inhibit production or activity ERα for treatment receptor-positive (ER
The mcr-1 gene was detected in 5.11% (58/1136) of Escherichia coli isolates chicken origin from 13 provinces China. A novel variant, named mcr-1.3, encoding an Ile-to-Val functional variant MCR-1 identified a sequence type 155 (ST155) strain. An mcr-1.3-containing IncI2 plasmid, pHeN867 (60,757 bp), identified. transfer led to 32-fold increase the MIC colistin recipient, exhibiting effect on resistance that similar mcr-1.
Objective: The purpose of the current study is to investigate whether texture analysis-based machine learning algorithms could help devise a non-invasive imaging biomarker for accurate classification meningiomas using algorithms. Method: cohort was established from hospital database by reviewing medical records. Patients were selected if they underwent meningioma resection in neurosurgery department between January 2015 and December 2018. A total number 40 parameters extracted pretreatment...
Phylogenetic relationships and lineage diversification of the family Salicaceae sensu lato (s.l.) remain poorly understood. In this study, we examined phylogenetic between 42 species from six genera based on complete plastomes. analyses 77 protein coding genes plastomes produced good resolution interrelationships among most sampled recovered clades. Of family, Flacourtia was identified as basal successive clades comprised both Itoa Poliothyrsis, Idesia, two stricto (s.s.) (Populus Salix)....
Genetic variations from few chloroplast DNA fragments show lower discriminatory power in the delimitation of closely related species and less resolution ability discerning interspecific relationships than nrITS. Here we use Orychophragmus (Brassicaceae) as a model system to test hypothesis that whole genomes (plastomes), with accumulation more despite slow evolution, can overcome these weaknesses. We used Illumina sequencing technology via reference-guided assembly construct complete...
A key limitation of the widely used CRISPR enzyme S. pyogenes Cas9 is strict requirement an NGG protospacer-adjacent motif (PAM) at target site. This constraint can be limiting for genome editing applications that require precise positioning. Recently, two variants with a relaxed PAM (NG) have been developed (xCas9 and Cas9-NG), but their activity has measured only small number endogenous sites. Here, we devise high-throughput pooled competition screen to compare performance thousands...
Abstract The energy metabolic rearrangement of triple‐negative breast cancer (TNBC) from oxidative phosphorylation to aerobic glycolysis is a significant biological feature and can promote the malignant progression. However, there little knowledge about functional mechanisms methyltransferase‐like protein 14 (METTL14) mediated contributes TNBC Our study found that METTL14 expression was significantly upregulated in tissues cell lines. Silencing inhibited growth invasion vitro, as well...
Abstract Atherosclerotic cardiovascular disease remains a preeminent cause of morbidity and mortality globally. The onset atherosclerosis underpins the emergence ischemic diseases, including coronary heart (CHD). Its pathogenesis entails multiple factors such as inflammation, oxidative stress, apoptosis, vascular endothelial damage, foam cell formation, platelet activation. Furthermore, it triggers activation diverse signaling pathways Phosphatidylinositol 3‐kinase/protein kinase B...
Using an Atomic Force Microscope (AFM) with a 5.3 μm diameter spherical probe, we determined mechanical properties of individual human mammary epithelial cells. The cells were derived from pair cell lines that mimic progression through four phases neoplastic transformation: normal (non-transformed), immortal, tumorigenic, and metastatic. Measurements on in all taken over both the cytoplasmic nuclear regions. Moreover, measurements made for different microenvironments as related to cell–cell...
OSCA/TMEM63 channels, which have transporter-like architectures, are bona fide mechanosensitive (MS) ion channels that sense high-threshold mechanical forces in eukaryotic cells. The activation mechanism of these is not fully understood. Here we report cryo-EM structures a dimeric pore mutant OSCA1.1-F516A with sequentially extracellular dilated detergent environment. These suggest the sequential dilation resembles flower blooming and couples to contraction each monomer subunit towards dimer...
Three-dimensional (3D) NAND flash memory has become quite popular and is now widely used in data centers mobile devices due to its outstanding storage density cost-effectiveness. Larger capacity made possible by 3D quad-level cell (QLC) with the charge-trap (CT) structure, which stores four bits each cell. However, reliability sacrificed exchange for greater capacity. The lifespan of retention crucial non-volatile storage. Thus, an important role played Arrhenius model, prediction...
In the age of artificial intelligence, large language models (LLM) require rapid development along with massive volumes training data and parameter storage. Over past decade, 3D NAND flash memory has emerged as dominant non-volatile technology due to its high bit density capacity. However, because vertical stacking technique array designs, more complicated loss mechanisms compared 2D memory. As densities rise Quad-level-cells (QLC), small read margins will further complicate make situation...
The effects of three types feed, purchased from online stores and having similar prices, on the growth performance culture environment crucian carp (Carassius auratus) were studied in this experiment, which aimed to provide a reference for evaluation selection fish feed. results showed that feeding different feeds had significant effect carp, growth-promoting HD feed (feed produced by Haida Company Limited) was significantly better than other two feeds. For example, weight gain rate (WGR)...
In this study, a quantum mechanics/molecular mechanics (QM/MM) framework combined with the CASPT2//CASSCF approach was used to investigate excited-state decay and isomerization of rsKiiro green fluorescent protein (GFP) from its neutral "OFF" trans state. Upon irradiation at 400 nm, conformation is initially excited bright S1 A rapid state then occurs ultimately leads molecule ground Notably, clockwise counterclockwise rotations C8C9C11N12 [or C5C8C9C11] dihedral angle are asymmetric or...
The evolution of the landslide erosion bank slopes in reservoir regions is crucial for disaster prevention and mitigation hydropower projects, it also an important topic to investigate impact anthropogenic activities on environment. A dispute exists under impoundment. In this paper, we chose Xiluodu as a typical case analysis. located eastern Qinghai–Tibetan Plateau ranks fourth largest station world. Firstly, identified landslides using multi-temporal image interpretation method before...
Abstract Cancer cells are addicted to polyamines, polycations essential for cellular function. While dual targeting of polyamine biosynthesis and uptake is under clinical investigation in solid cancers, preclinical studies into its potential haematological malignancies lacking. Here we investigated the efficacy depletion acute leukaemia. The inhibitor difluoromethylornithine (DFMO) inhibited growth a molecularly diverse panel leukaemia cell lines, while non‐malignant were unaffected....