A5062637395- Vascular Malformations and Hemangiomas
- Respiratory Support and Mechanisms
- Vascular Malformations Diagnosis and Treatment
- Tumors and Oncological Cases
- Acute Kidney Injury Research
- Vascular Tumors and Angiosarcomas
- Nosocomial Infections in ICU
- Pediatric health and respiratory diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Sepsis Diagnosis and Treatment
- Electrolyte and hormonal disorders
- Immune Response and Inflammation
- Chronic Kidney Disease and Diabetes
- Renal Transplantation Outcomes and Treatments
- Cell death mechanisms and regulation
- Teratomas and Epidermoid Cysts
- Dialysis and Renal Disease Management
- Histiocytic Disorders and Treatments
- Renal and related cancers
- Renal function and acid-base balance
- Respiratory viral infections research
- Neuroscience of respiration and sleep
- Tuberous Sclerosis Complex Research
- Renal Diseases and Glomerulopathies
- Trauma, Hemostasis, Coagulopathy, Resuscitation
University of Washington
2017-2024
Seattle Children's Hospital
2021-2023
Harborview Medical Center
2017-2021
Pulmonary and Critical Care Associates
2018-2019
SleepMed
2018
Currently, no safe and effective pharmacologic interventions exist for acute kidney injury (AKI). One reason may be that heterogeneity exists within the AKI population, thereby hampering identification of specific pathophysiologic pathways therapeutic targets.The aim this study was to identify test whether subphenotypes have prognostic implications.First, latent class analysis methodology applied independently in two critically ill populations (discovery [n = 794] replication 425]) with AKI....
Neutrophils release neutrophil extracellular traps (NETs) in response to invading pathogens. Although NETs play an important role host defense against microbial pathogens, they have also been shown a contributing mechanistic pathologic inflammation the absence of infection. for bacterial pneumonia and acute respiratory distress syndrome (ARDS) is emerging, comprehensive evaluation alveolar space critically ill patients has yet be reported. In this study, we evaluated whether markers NET...
The relationship between the PD-L1 (Programmed Death-Ligand 1)/PD-1 pathway, lung inflammation, and clinical outcomes in acute respiratory distress syndrome (ARDS) is poorly understood. We sought to determine whether PD-L1/PD-1 or blood associated with ARDS severity. measured soluble (sPD-L1) plasma lower tract samples (ARDS1 [
Visual Abstract Export Background and objectives Critically ill patients with worsening AKI are at high risk for poor outcomes. Predicting which will experience progression of remains elusive. We sought to develop validate a model predicting severe within 72 hours after intensive care unit admission. Design, setting, participants, & measurements applied least absolute shrinkage selection operator regression methodology two prospectively enrolled, critically cohorts who met criteria the...
Abstract Background We previously identified two acute kidney injury (AKI) sub-phenotypes (AKI-SP1 and AKI-SP2) with different risk of poor clinical outcomes response to vasopressor therapy. Plasma biomarkers endothelial dysfunction (tumor necrosis factor receptor-1, angiopoietin-1 2) differentiated the AKI sub-phenotypes. However, it is unknown whether these are simply markers or causal mediators in development Methods tested for associations between single-nucleotide polymorphisms within...
Somatic activating variants in PIK3CA, the gene that encodes p110α catalytic subunit of phosphatidylinositol 3-kinase (PI3K), have been previously detected ∼80% lymphatic malformations (LMs).1,2 We report presence somatic BRAF individuals with LMs do not possess pathogenic PIK3CA variants. The substitution p.Val600Glu (c.1799T>A), one most common driver mutations cancer, was multiple LMs. Histology revealed abnormal channels immunopositivity for BRAFV600E endothelial cells otherwise...
Critically ill patients with acute kidney injury (AKI) can be divided into two subphenotypes, resolving or nonresolving, on the basis of trajectory serum creatinine. It is unknown if biology underlying these AKI recovery patterns different. We measured eight circulating biomarkers in plasma obtained from a cohort admitted to an intensive care unit (ICU) (n = 1241) systemic inflammatory response syndrome. The were representative several biologic processes: apoptosis (soluble Fas),...
CD4+ T-helper 17 (Th17) cells and Interleukin (IL)-17A play an important role in clearing pathogens mouse models of pneumonia. We hypothesized that numbers Th17 levels IL-17A are associated with risk for nosocomial pneumonia humans.We collected bronchoalveolar lavage (BAL) fluid from mechanically ventilated (n = 25) patients undergoing quantitative bacterial culture to evaluate ventilator (VAP). identified by positive selection cells, stimulation ionomycin PMA, then staining CD4, CD45, CCR6,...
Disorganized morphogenesis of arteries, veins, capillaries, and lymphatic vessels results in vascular malformations. Most individuals with isolated malformations have postzygotic (mosaic), activating pathogenic variants a handful oncogenes within the PI3K–RAS–MAPK pathway (Padia et al., Laryngoscope Investig Otolaryngol 4: 170–173 [2019]). Activating gene PIK3CA , which encodes for catalytic subunit phosphatidylinositol 3-kinase, are present both venous as well arteriovenous other complex...
Lymphatic malformations (LMs) are congenital anomalies of the lymphatic vasculature that affect approximately 1:4,000 live births and consist dilated channels surrounded by hypertrophic stroma, resulting in a compressible lesion with mass effect. Somatic activating single nucleotide variants (SNVs) PIK3CA, which encodes p110α catalytic subunit phosphoinositide 3-kinase, have been identified 80% LMs localized to LM endothelial cells (LM-ECs) (Boscolo et al., 2015Boscolo E. Coma S. Luks V.L....
OBJECTIVES: Multiple organ failure in critically ill patients is associated with poor prognosis, but biomarkers contributory to pathogenesis are unknown. Previous studies support a role for Fas cell surface death receptor (Fas)-mediated apoptosis dysfunction. Our objectives were test associations between soluble and multiple failure, identify protein quantitative trait loci, determine genetic variants failure. DESIGN: Retrospective observational cohort study. SETTING: Four academic ICUs at...
Supplemental data file that includes supplementary tables referenced in the text. (DOCX 32 kb)
Abstract Somatic activating variants in PIK3CA , the gene that encodes p110 α catalytic subunit of PI3K, have been previously detected ∼80% lymphatic malformations (LM). 1; 2 We report presence somatic BRAF individuals with -negative LM. The substitution p.Val600Glu (c.1799T>A), one most common driver mutations cancer, was multiple Histology revealed abnormal channels immunopositivity for V600E endothelial cells otherwise indistinguishable from positive finding contribute to low-flow LMs...