A5075257855- Bacterial Identification and Susceptibility Testing
- Helicobacter pylori-related gastroenterology studies
- Streptococcal Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Antibiotic Resistance in Bacteria
- Eosinophilic Esophagitis
- Urinary Tract Infections Management
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Veterinary medicine and infectious diseases
- Oral microbiology and periodontitis research
- Microbial infections and disease research
- Antibiotics Pharmacokinetics and Efficacy
- Sepsis Diagnosis and Treatment
- Bacterial Infections and Vaccines
- Nephrotoxicity and Medicinal Plants
- Neutropenia and Cancer Infections
- Rabies epidemiology and control
- Gastric Cancer Management and Outcomes
- Inflammation biomarkers and pathways
Centre Hospitalier Régional et Universitaire de Nancy
2014-2025
Université de Lorraine
2013-2024
Laboratoire de Chimie Physique et Microbiologie pour l'Environnement
2024
Architecture, Territoire, Environnement
2023
Inserm
2008-2023
Stress, immunité, pathogènes
2014-2022
Service de la Santé Publique
1999-2022
ImmunoGenes (Hungary)
2014-2019
Centre National de la Recherche Scientifique
1999-2010
Centre Hospitalier Universitaire de Montpellier
2010
Gastric infection with Helicobacter pylori is a cosmopolitan problem, and especially common in developing regions where there also high prevalence of gastric cancer. These infections are known to cause gastritis peptic ulcers, dramatically enhance the risk Eradication this organism an important medical goal that complicated by development resistance conventional antimicrobial agents persistence low level reservoir H. within epithelial cells. Moreover, economic practical problems preclude...
The triggering receptor expressed on myeloid cells (TREM)-1 is a recently discovered the surface of neutrophils and subset monocytes. Engagement TREM-1 has been reported to trigger synthesis proinflammatory cytokines in presence microbial products. Previously, we have identified soluble form (sTREM-1) observed significant levels serum samples from septic shock patients but not controls. Here, investigated its putative role modulation inflammation during sepsis. We that sTREM-1 was secreted...
The incidence of nontuberculous mycobacteria (NTM) pulmonary diseases in HIV-negative patients was studied prospectively from January 1, 2001 to December 31, 2003 by 32 sentinel sites distributed throughout France. In total, 262 who yielded NTM isolates respiratory clinical specimens, met the bacteriological, radiological and criteria established American Thoracic Society for disease. Among isolates, 234 were slow-growing (125 Mycobacterium avium-intracellulare complex (MAC), 66 M. xenopi ,...
Previous studies have suggested that procalcitonin is a reliable marker for predicting bacteremia. However, these had relatively small sample sizes or focused on single clinical entity. The primary endpoint of this study was to investigate the diagnostic accuracy excluding clinically relevant pathogen categories in patients with suspected bloodstream infections. secondary look organisms significantly associated internationally validated intervals. We performed cross-sectional included 35,343...
The antibacterial activity of sulforaphane [4-(methylsulfinyl)butyl isothiocyanate] has been previously described. We have analysed the activities 12 isothiocyanates (ITC) including sulphoraphane on 25 strains Helicobacter pylori using an agar dilution assay. In addition, bactericidal effects against H. were determined for 6 most active ITCs, both directly and intracellular bacteria in cultured human epithelial (HEp-2) cells. MIC90 values these ITCs ranged between 4 32 μg/mL four potent...
Triggering receptor expressed on myeloid cells-1 (TREM-1) is a cell-surface molecule that has been identified both human and murine polymorphonuclear neutrophils mature monocytes. The activation of TREM-1 in the presence microbial components amplifies inflammatory response may be responsible for hyperresponsiveness observed during initial stage sepsis. aim present study was to investigate effect modulation pathway experimental pneumonia rats.Adult male Wistar rats were intratracheally...
Mupirocin is the cornerstone of decolonization regimens, a successful strategy to prevent healthcare-associated staphylococcal infections. Several recent studies have reported alarming results: (i) an extending reservoir mupA, ancestral mobile resistance gene, among coagulase-negative staphylococci (CoNS); (ii) emergence new gene (mupB); and (iii) growing number mupirocin-resistant methicillin-resistant Staphylococcus aureus (MRSA), including highly pathogenic clones. We performed nationwide...
Current recommendations for empirical antimicrobial therapy in spontaneous bacterial peritonitis (SBP) are based on quite old trials. Since microbial epidemiology and the management of patients have changed, whether these still appropriate must be confirmed. An observational study that exhaustively collected clinical biological data associated with positive ascitic fluid cultures was conducted four French university hospitals 2010–2011. Two hundred sixty-eight documented were observed 190...
The objective of this study was to determine the effects a TREM (triggering receptor expressed on myeloid cells 1)–like transcript 1–derived peptide (LR12) administration during septic shock in pigs. Two hours after induction fecal peritonitis, anesthetized and mechanically ventilated adult male minipigs were randomized receive LR12 (n = 6) or its vehicle alone (normal saline, n 5). animals operated instrumented without peritonitis served as controls (sham). Resuscitation achieved using...
Spontaneous bacterial peritonitis (SBP) is a frequent and life-threatening complication of cirrhosis, contributing to considerable morbidity mortality. A cross-sectional derivation study was conducted assess the diagnostic accuracy two sepsis-related calcitonin peptide family biomarkers, mid-regional pro-adrenomedullin (MR-pro-ADM) procalcitonin, in ascitic fluid for identifying bacteriologically confirmed SBP (BC-SBP). In subsequent validation study, performance 'SBP score' evaluated an...
ABSTRACT Sulforaphane, an isothiocyanate abundant in the form of its glucosinolate precursor broccoli sprouts, has shown vitro activity against Helicobacter pylori . We evaluated effect sulforaphane vivo this bacterium by using human gastric xenografts nude mice. H. was completely eradicated 8 11 sulforaphane-treated grafts. This result suggests that might be beneficial treatment -infected individuals.
ABSTRACT Nonduplicate clinical isolates of Prevotella spp. recovered from patients hospitalized between 2003 and 2006 in two French tertiary-care teaching hospitals were investigated for their susceptibility to metronidazole the presence nim genes. Of 188 strains tested, 3 displayed reduced after 48 h incubation, while 27 additional exhibited heterogeneous resistance prolonged incubation; all 30 negative. Among remaining 158 isolates, 7 -positive detected. All these identified as baroniae by...
qnrD is a plasmid mediated quinolone resistance gene from unknown origin, recently described in Enterobacteriaceae. It encodes pentapeptide repeat protein 36–60% different the other Qnr (A, B, C, S and VC). Since most qnrD-positive strains were as belonging to Proteus or Providencia genera, we hypothesized that originated Proteeae before disseminating enterobacterial species. We screened 317 of for its genetic support by PCR. For all seven (4 mirabilis, 1 vulgaris 2 rettgeri) was carried...
Detection of Clostridium difficile toxins A and B in stools by Premier Cytoclone A+B enzyme immunoassay (EIA) was compared with detection stool culture for C. followed toxigenic isolates using the same EIA. Chart reviews were performed to evaluate likelihood difficile-associated diarrhea colitis (CADC) all patients at least one positive toxin assay. While detected 58 85 consecutive CADC both assays, 5 only assay, 22 culture. Our results suggest that laboratories a rapid EIA, direct should be...
A hitherto unknown anaerobic bacillus isolated from sinus pus in a young child (strain AIP 354.02T) was characterized by using phenotypic and genotypic methods. 16S rRNA gene sequence analysis indicated that this strain phylogenetically affiliated with several sequences of cloned inserts previously deposited the public databases. According to their similarities, these uncultivated bacteria, together 354.02T, formed separate subgroup belonging family 'Lachnospiraceae' within phylum...
Objectives: The triggering receptor expressed on myeloid cells (TREM)-1, a the surface of neutrophils and monocytes/macrophages, synergizes with Toll-like receptors in amplifying inflammatory response mediated by microbial components. Because pathogenesis ischemia-reperfusion-induced gastrointestinal tissue injury multiple organ failure implies leukocyte activation bacterial translocation, we hypothesized that TREM-1 pathway modulation would prove beneficial this setting. Design: Animal...
ABSTRACT Acetic acid bacteria (AAB) are broadly used in industrial food processing. Among them, members of the genera Asaia , Acetobacter and Granulibacter were recently reported to be human opportunistic pathogens. We isolated AAB from clinical samples three patients describe here bacteriological features these cases. report for first time (i) isolation a Gluconobacter sp. samples; (ii) successive different AAB, i.e., an two unrelated spp., cystic fibrosis patient; (iii) persistent...
In this work, we characterized a new, 160-kb, blaOXA-48-harboring IncL/M-type plasmid isolated from Klebsiella pneumoniae strain France. Moreover, report the transfer of 60-kb OXA-48-encoding to other Enterobacteriaceae in two patients.