Dengue infections are still a worldwide burden, especially in Indonesia. There is no specific medication against the dengue virus. Recently, many types of research have been conducted to discover new drug for virus using natural resource extracts. Indonesia, as tropical country, has wide biodiversity. several medicinal plants Indonesia that believed possess anti-dengue activity, such Myristica fatua, Cymbopogon citratus, and Acorus calamus plants. We an vitro laboratory experiment extracts...

Gut dysbiosis has a role in the pathogenesis of lupus. Synbiotic supplementation may restore balance gut microbiota. This study investigated whether synbiotics could improve microbiota and systemic inflammation lupus patients. randomized, double-blind, placebo-controlled trial was conducted adult erythematosus (SLE) Subjects were randomized to receive either or placebo. Fecal microbiota, hs-CRP, IL-6, IL-17 measured at baseline after 60 days. Patients who fulfilled inclusion criteria into...

Dengue virus (DENV) infection is still a global health problem. The severity of DENV related to the high viral load and cytokine storm caused by excessive inflammation. There no specific antiviral used for DENV. Meanwhile, use anti-inflammatory drugs limited patients with severe clinical symptoms. Interestingly, favipiravir (FVP) quinine sulfate (QS) have been reported as repurposing that can inhibit replication. However, their activity in has not studied yet. Thus, current study aimed...

Dengue is the most common infective disease caused by dengue virus (DENV) and endemic diseases in tropical subtropical areas. Until now, there no specific antiviral for infection. It known that viral load related to severity. Curcuma longa L. (turmeric) with curcumin as major active compound has been identified its effect. This study determine effect of C. extract on DENV-2 vitro vivo along toxicity liver kidney ddY mice. Antiviral activity (IC50) (CC50) was examined Huh7it-1 cells focus...

Plasma leakage is one of the characteristic features dengue haemorrhagic fever. The interaction among peripheral blood mononuclear cells (PBMCs), virus and endothelial was analysed in vitro. Human umbilical vein (HUVECs) were infected with dengue-2 (DV-2) at an m.o.i. 0.5 p.f.u. per cell. PBMCs added to DV-2-infected HUVECs, transendothelial electrical resistance (TEER) transalbumin permeability assessed. Dengue infection cell alone did not decrease TEER, but addition decreased increased...

Background Dengue virus (DENV) infection causes viral haemorrhagic fever that is characterized by extensive activation of the immune system. The aim this study to investigate kinetics transcriptome signature changes during course disease and association genes in these signatures with clinical parameters. Methodology/Principle Findings Sequential whole blood samples from DENV infected patients Jakarta were profiled using affymetrix microarrays, which analysed principal component analysis,...

Objectives Plasma leakage, a hallmark of disease in Dengue virus (DENV) infection, is an important clinical manifestation and often associated with numerous factors such as viral factors. The aim this study to investigate the association serotype, load kinetics, history NS1 protein plasma leakage. Methods Subjects fever ≤ 48 hours positive DENV infection were included. Serial laboratory tests, measurements, ultrasonography examination assess leakage performed. Results DENV-3 was most common...

Infection with hepatitis C virus (HCV) results in C, a disease characterized by chronic infection, cirrhosis, and hepatocellular carcinoma. Currently, the standard therapy is combination of pegylated interferon-α plus ribavirin NS3 protease inhibitors. Addition inhibitors to improves response rates; however, use also associated significant adverse effects an increase overall cost treatment. Therefore, there need develop safe inexpensive drugs for treatment HCV infections. In this study, we...

In Indonesia, dengue has become one of the hyperendemic diseases. Dengue consists three clinical phases—febrile phase, critical and recovery phase. Many patients have died in phase due to lack proper timely treatment. Therefore, we developed models that can predict severity level based on laboratory test results corresponding using Artificial Neural Network (ANN) Discriminant Analysis (DA). developing models, used a very small dataset. It is shown ANN logistic hyperbolic tangent activation...

Emergence of multidrug-resistant Neisseria gonorrhoeae resulting from new genetic mutations is a serious threat to controlling gonorrhea.To determine 1) antimicrobial susceptibilities and the corresponding 2) role MtrRCDE efflux system in gonococcal resistance fluoroquinolones.Antimicrobial susceptibility testing sequence analysis gyrA, parC, mtrR loci 131 N. isolates Japan.The proportion strains resistant intermediate-resistant antimicrobials was 25.2% 48.9% for ciprofloxacin, 30.5%...

Latar Belakang: Demam Berdarah Dengue (DBD) yang disebabkan oleh virus dengue masih merupakan masalah utama di negara tropis. Sampai saat ini belum tersedia vaksin maupun terapi efektif untuk DBD. Oleh karena itu, penelitian menemukan antivirus spesifik sangat diperlukan. Indonesia kaya akan tumbuhan herbal mungkin berpotensi memiliki aktivitas antivirus, diantaranya adalah Psidium guajava (Jambu biji), Euphorbia hirta (Patikan kerbau), Piper bettle L. (Sirih), Carica papaya (Pepaya),...

Dengue is one of the endemic diseases in Indonesia. being suffered by many people, regardless their gender and age. Therefore, research about dengue based on patients' data was conducted. There a lot information written that regarding corresponding patients they had suffered, such as gender, age, how long were hospitalized, symptoms experienced, laboratory characteristics results. Diagnosis each results also data. The diagnoses classified into three different clinical degrees according to...

Background and Objectives: Dengue viruses (DENV) is a mosquito-borne members of the Flaviridae family.To date, more than 2.5 billion people in over 100 countries are at risk infection, approximately 20 million infections were reported annually. Currently, There no specific antiviral treatment available for DENV infection. Natural products possess wide range biological biochemical potential. Among them, plants one most important sources discovering new drugs therapy. Our previous study,...

Dengue is an acute febrile disease caused by dengue virus (DENV) that transmitted Aedes sp., which causes serious health conditions in many countries. Non-structural protein 1 (NS1) a co-factor for the RNA replication of this virus, represents new strategy identification dengue. Prompt and accurate laboratory diagnosis infection required to assist patient triage management, as well prevent spread infection. In present study, we tested potential surface plasmon resonance (SPR) diagnostic tool...

Nickel foam modified by hollow sphere NiCo 2 O 4 particles was successfully prepared via a hydrothermal method. Investigation of the -modified nickel as an electrode for detection glucose in sodium hydroxide solution showed high linearity anodic currents.

Background: Since the beginning of coronavirus disease 2019 (COVID-19) outbreak, dynamic mutations in receptor-binding domain (RBD) severe acute respiratory syndrome 2 (SARS-CoV-2) spike protein have altered pathogenicity variants virus circulating Indonesia. This research analyzes mutation trend various RBD samples from Indonesia published Global Initiative on Sharing All Influenza Data (GISAID) database using genomic profiling. Method: Patients infected with SARS-CoV-2, whose been...

The development of a dengue virus vaccine is major priority in efforts to control the diseases. Several researchers are currently using Asian 1 and 2 genotypes as candidates for type (DENV-2). However, this study, we constructed recombinant plasmid-based prM/E gene, from DENV-2 Cosmopolitan genotype strain DNA candidate. protein expression plasmid CHO cells was analyzed an enzyme-linked immunosorbent assay, western blotting, sucrose gradient sedimentation. After being used immunize ddY mice...

Dengue virus (DENV) is one of the most common viral infections found Indonesia and tropical regions, no specific antiviral for DENV. has several herbal medicine that were not explored their potency as This study was done to evaluate activity toxicity 4 derived fractions: Hexane (CA1), ethyl acetate (CA2), buthanol (CA3 ) water (CA4) Cassia alata leaf extract (CA) an drug The DENV treated with various concentration added Huh-7 it-1. decrease titer determined by Focus assay. measured MTT In...

Abstract The prevalence of dengue virus infection (DENV) in Indonesia is still high compared to other tropical countries the world. Unfortunately, specific antiviral drug DENV not available yet. pure compound such as quercetin revealed a good candidat. Quercetin plant-derived flavonoid that can be found wide variety fruits and vegetables. It has been proven able improve body performance reduce risk well inhibit replication Vero cell. However, research on human cell line yet conducted....

Dengue hemorrhagic fever (DHF) is major public health problem in tropical and subtropical areas of the world with lack approved vaccines effective antiviral therapies. With no current treatment for illness attributed to dengue virus (DENV) infection other than supportive care, therapeutic strategies that use natural extract was developed. Indonesia have many plants potential drµgs such as Pterocarpus indicus Willd (P. indicus). The objective this study determine effect P. inhibit DENV...

Latar Belakang: Demam Berdarah Dengue (DBD) yang disebabkan oleh virus dengue masih merupakan masalah utama di negara tropis. Sampai saat ini belum tersedia vaksin maupun terapi efektif untuk DBD. Oleh karena itu, penelitian menemukan antivirus spesifik sangat diperlukan. Indonesia kaya akan tumbuhan herbal mungkin berpotensi memiliki aktivitas antivirus, diantaranya adalah Psidium guajava (Jambu biji), Euphorbia hirta (Patikan kerbau), Piper bettle L. (Sirih), Carica papaya (Pepaya),...