Alzheimer disease (AD) is an age-related neurodegenerative disorder characterized by the presence of senile plaques, neurofibrillary tangles and neuronal loss. Amyloid-β protein (Aβ) deposition plays a critical role in development AD. It now generally accepted that massive death due to apoptosis common characteristic brains patients suffering from diseases, apoptotic cell has been found neurons glial cells Melatonin secretory product pineal gland; melatonin potent antioxidant free radical...

To observe the effects of electroacupuncture (EA) on anxiety-like behavior, learning and memory ability expressions myelin-related proteins in hippocampus senescence accelerated mice prone 8 (SAMP8), so as to explore mechanism EA improving Alzheimer's disease (AD) from perspective myelin sheath injury regeneration. Twenty-four male SAMP8 were randomly equally divided into model group group. Twelve SAMR1 same age used control The rats received acupuncture at "Baihui" (GV20), "Dazhui" (GV14)...

Background:Aging is a highly complex process that affects various tissues and systems in the body. Senescent changes are relatively more prevalent severe postmitotic cells. Mitochondria play an important role aging process. Recently, cell cultures have been widely used as vitro model to study aging. The present was designed investigate mitochondrial dysfunction associated with long-term culture system.Material/Methods:Rat hippocampal neurons were maintained serum-free medium for 30 days...

Melatonin mediates a variety of biological processes ranging from the control circadian rhythms to immune regulation. also influences bone formation and osteointegration dental implants. However, effects melatonin on dentine have not been examined. This study investigated proliferation differentiation rat papilla cells (rDPCs) in vitro vivo. We found that (0, 10(-12) , 10(-10) ,10(-8) m) induced dose-dependent reduction rDPCs proliferation, increased alkaline phosphatase (ALP) activity,...

Modulation of the amyloid-β (Aβ) trafficking pathway heralds a new therapeutic frontier for Alzheimer's disease (AD). As CD74 binds to precursor protein (APP) and can suppresses Aβ processing, we investigated whether recombinant adeno-associated virus (AAV) delivery could reduce production affect outcomes. This idea was tested in mouse AD model. Cotransduction AAV-tetracycline-controlled transactivator (tTA) AAV-tet-response element (TRE)-CD74 resulted expression, reduced neurons containing...

Abnormalities of brain energy metabolism are involved in Alzheimer disease (AD). Sirtuin 1 (SIRT1) is a class III histone deacetylase and activates peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α), which enhances mitochondrial biogenesis homeostasis. Electroacupuncture (EA) has been reported to improve AD. However, the effect EA on SIRT1 PGC-1α AD remains unclear.ATP levels were measured using assay kits hippocampus frontal cortex senescence-accelerated mouse prone 8...

beta-Amyloid (Abeta) is strongly involved in the pathogenesis of Alzheimer's disease (AD), and mitochondria play an important role neurodegenerative disorders. To determine whether any different effect melatonin on cultured neurons treated with Abeta vitro which may be produced through its action at stages culture, we investigated damage rat hippocampal mitochondrial function induced by young [days 10 (DIV 10)] senescent 25) protective melatonin. Rat were incubated amyloid-beta peptide 25-35...

Amyloid-β (Aβ)-stimulated microglial inflammatory responses engage mitogen-activated protein kinase (MAPK) pathways in Alzheimer's disease (AD). Mixed-lineage kinases (MLKs) regulate upstream MAPK signaling that include p38 and c-Jun amino-terminal (JNK). However, whether MLK-MAPK affect Aβ-mediated neuroinflammation is unknown. To this end, we investigated if URMC-099, a brain-penetrant small-molecule MLK type 3 inhibitor, can modulate Aβ trafficking processing required for generating...

<i>Background:</i> Neuronal and synaptic function in Alzheimer’s disease (AD) is measured vivo by glucose metabolism using positron emission tomography (PET). <i>Objective:</i> We hypothesized that neuronal activation as PET a more sensitive index of dysfunction than activity during rest. investigated if the correlations between dementia severity with Mini Mental State Examination (MMSE) are an artifact brain atrophy. <i>Method:</i> Glucose was...

Synaptic loss and dysfunction is associated with cognitive impairment in Alzheimer’s disease (AD). Recent evidence indicates that the AMP-activated protein kinase (AMPK)/eukaryotic elongation factor-2 (eEF2K)/eukaryotic (eEF2) pathway was implicated synaptic plasticity AD. Therapeutic strategies for AD treatment are currently limited. Here, we investigate effects of electroacupuncture (EA) on function AMPK/eEF2K/eEF2 signaling male senescence-accelerated mouse-prone 8 (SAMP8) mice. Male...

Electroacupuncture (EA) has been reported to have beneficial effects on Alzheimer's disease (AD). BACE1 (β-site amyloid precursor protein-cleaving enzyme 1) is involved in the abnormal production of amyloid-β plaque (Aβ), a hallmark AD pathophysiology. Thus, present study investigated EA memory impairment, Aβ production, and expression senescence-accelerated mouse prone 8 (SAMP8) mice. We found that improved spatial learning impairment SAMP8 Furthermore, attenuated repressed hippocampus...

The role of Corydalis decumbens (CD) in macrophage activation remains unclear, particularly the Ras homolog family member A (RhoA) signaling pathway. Therefore, present study aimed to investigate effect CD on viability, proliferation, morphological changes, migration, phagocytosis, differentiation, and release inflammatory factors pathways lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages.Cell counting kit-8 water-soluble tetrazolium salt assays were used evaluate viability...

To explore the effect of early intervention electroacupuncture (EA) at "Baihui" (GV 20), "Dazhui" 14) and "Shenshu" (BL 23) on learning-memory ability expression phosphorylated Tau protein in hippocampus SAMP8 mice, so as to provide reference for intervening period EA Alzheimer's disease (AD).A total 36 3-month old mice were randomly divided into a model group, 3-month-old group 9-month-old 12 each group. Twelve normal SAMR1 with same age taken control The treated separately 3 months 9...

To observe the effect of electroacupuncture (EA) on expression Iba-1, complement C1q and CD68 in hippocampus SAMP8 mice, so as to explore its mechanisms underlying improvement Alzheimer's disease (AD).Twenty-four male mice were randomly equally divided into model EA groups, 12 SAMR1 used control group. (2 Hz, 1.5-2.0 mA) was applied "Baihui" (GV20), "Dazhui"(GV14) "Shen-shu"(BL23) for 20 min once daily group, each course treatment 8 days, with an interval 2 days between two courses, treated...