A5056066740- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- HIV Research and Treatment
- RNA Research and Splicing
- Immune Cell Function and Interaction
- Genetics and Physical Performance
- Ion channel regulation and function
- Viral-associated cancers and disorders
- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Cardiac Arrest and Resuscitation
- Coenzyme Q10 studies and effects
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- Graphene and Nanomaterials Applications
- Hypothalamic control of reproductive hormones
- Dermatology and Skin Diseases
- Muscle metabolism and nutrition
- Cardiovascular and exercise physiology
- Cytomegalovirus and herpesvirus research
- Hormonal and reproductive studies
- Neuroscience and Neuropharmacology Research
- Nuclear Structure and Function
Amgen (United States)
2022-2024
University of California, San Francisco
2017-2018
University of California, Berkeley
2006-2013
Cleveland Clinic Lerner College of Medicine
2008
University of California, Davis
2007
Integra (United States)
2006
We hypothesized that in addition to serving as a fuel source and gluconeogenic precursor, lactate anion (La-) is signaling molecule. Therefore, we screened genome-wide responses of L6 cells elevated (10 20 mM) sodium-La- added buffered, high-glucose media. Lactate increased reactive oxygen species (ROS) production up-regulated 673 genes, many known be responsive ROS Ca2+. The induction genes encoding for components the mitochondrial oxidation complex was confirmed by independent methods (PCR...
Results of previous studies suggested a role mitochondria in intracellular and cell-cell lactate shuttles. Therefore, by using rat-derived L6 skeletal muscle cell line confocal laser-scanning microscopy (CLSM), we examined the cellular locations mitochondria, dehydrogenase (LDH), lactate-pyruvate transporter MCT1, CD147, purported chaperone protein for MCT1. CLSM showed that LDH, CD147 are colocalized with mitochondrial reticulum. Western blots cytochrome oxidase (COX), NADH dehydrogenase,...
To evaluate the presence of components a putative Intracellular Lactate Shuttle (ILS) in neurons, we attempted to determine if monocarboxylate (e.g. lactate) transporter isoforms (MCT1 and -2) lactate dehydrogenase (LDH) are coexpressed neuronal mitochondria rat brains. Immunohistochemical analyses brain cross-sections showed MCT1, MCT2, LDH colocalize with mitochondrial inner membrane marker cytochrome oxidase (COX) cortical, hippocampal, thalamic neurons. Immunoblotting after...
We hypothesized that dysregulation of lactate/pyruvate (monocarboxylate) transporters (MCT) and lactate dehydrogenase (LDH) isoforms contribute to the Warburg effect in cancer. Therefore, we assayed for expression levels localizations MCT ( 1 , 2 4 ), LDH (A B) breast cancer cell lines MCF-7 MDA-MB-231 compared results with those from a control, untransformed primary line, HMEC 184. Remarkably, MCT1 is not expressed MDA-MB-231, but cells, where its abundance less than control 184 cells. When...
To characterize susceptibility to HIV infection, we phenotyped infected tonsillar T cells by single-cell mass cytometry and created comprehensive maps identify which subsets of CD4+ support fusion productive infection. By comparing HIV-fused HIV-infected through dimensionality reduction, clustering, statistical approaches account for viral perturbations, identified a subset memory that entry but not gene expression. These express high levels CD127, the IL-7 receptor, are believed be...
HIV-1-infected cells persist indefinitely despite the use of combination antiretroviral therapy (ART), and novel therapeutic strategies to target purge residual infected in individuals on ART are urgently needed. Here, we demonstrate that CD4+ T cell-associated HIV-1 RNA is often highly enriched expressing CD30, this marker considerably contribute total pool transcriptionally active lymphocytes suppressive ART. Using situ hybridization studies, show co-localization CD30 with transcriptional...
The evolutionarily conserved kelch-repeat protein muskelin was identified as an intracellular mediator of cell spreading. We discovered that its morphological activity is controlled by association with RanBP9/RanBPM, a involved in transmembrane signaling and complex. By subcellular fractionation, endogenous present both the nucleus cytosol. Muskelin localization coregulated C terminus, which provides cytoplasmic restraint also controls interaction RanBP9, atypical lissencephaly-1 homology...
Persistent tissue reservoirs of HIV present a major barrier to cure. Defining subsets infected cells in tissues is focus cure research. Herein, we describe novel multiplexed situ hybridization (ISH) (RNAscope) protocol detect HIV-DNA (vDNA) and HIV-RNA (vRNA) formalin-fixed paraffin-embedded (FFPE) human combination with immunofluorescence (IF) phenotyping the cells. We show that IF ISH (mIFISH) suitable for quantitative assessment vRNA vDNA multiparameter allows precise identification...
Shuttling of intermediary metabolites, such as pyruvate, contributes to the dynamic energy and biosynthetic needs tissues. Tracer kinetic studies offer a powerful tool measure metabolism substrates like pyruvate that are simultaneously taken up from released into circulation by organs. However, we understood during each circulatory passage, entire cardiac output transits pulmonary circulation. Therefore, examined transpulmonary kinetics in an anesthetized rat model unstimulated (Con),...
Due to their unique properties, engineered nanoparticles (NPs) have found broad use in industry, technology, and medicine, including as a vehicle for drug delivery. However, the understanding of NPs’ interaction with different types mammalian cells lags significantly behind increasing adoption In this study, we show responses human epithelial breast when exposed polymeric Eudragit® RS NPs (ENPs) 1–3 days. Cells displayed dose-dependent increases metabolic activity growth, but lower...
Abstract Atopic dermatitis (AD) is a common chronic inflammatory skin disorder characterized by disrupted epidermal barrier function and aberrant immune responses. Despite recent developments in new therapeutics for AD, there still large unmet medical need disease management due to the complex multifactorial nature of AD. Recent genome-wide association studies (GWAS) have identified NLRP10 as susceptible gene AD but physiological role homeostasis remains unknown. Here we show that...
PURPOSE: Lactate plays an important role in adaptive response of muscle to exercise. The possible contribution gene transcription causing lactate-induced responses remains be discovered. goal this study was assess genome-wide L6 myocytes lactate. METHODS: were cultured Dulbecco's modified Eagle's Medium (high glucose). Eight days following seeding the cells they incubated with media containing 0 mM or 20 sodium then harvested 1 hr 6 after incubation GeneChip analysis (Rat genome 230A2.0,...
Glucose-dependent insulinotropic polypeptide receptor (Gipr) gene expression has been reported in mouse spermatids and Gipr knockout male mice have previously to decreased vitro fertilization, although the role of signaling fertility is not well understood.The purposes these studies were determine glucose-dependent using anti-glucose-dependent antibody-treated wild-type if testes similar non-human human primates.Adiponectin promoter-driven (GiprAdipo-/- ) assessed for vivo fertility, sperm...
Studies of lactate metabolism in vivo humans and rodents have shown that is not only an end product glycolysis, but important fuel for active muscles other tissues. The operation shuttles within among cells, tissues organs such as retina, brain, testis, liver, cardiac skeletal muscle under fully aerobic conditions well established. Lactate produced significant amounts cancer cells due to enhanced described a "Warburg Effect," the role its transporters growth poorly understood. PURPOSE: In...
Exercise training, in which arterial blood lactate concentration rises, increases the expression of transporter MCT1 mammalian skeletal muscle. PURPOSE: In present study, we investigated whether accumulation by itself affects rat-derived L6 muscle cells. METHODS: The experiments were carried out on day 8 following seeding. cells incubated Dulbecco's modified Eagle's Medium (high glucose) containing 0 mM, 10 mM or 20 sodium at start experiment, and harvested 1h, 6h, 24h, 48h incubation medium...
Results of previous studies suggested a role mitochondria (MI) in an Intracellular Lactate Shuttle. Therefore, using confocal laser-scanning microscopy (CLSM) we examined the cellular locations mitochondria, lactate dehydrogenase (LDH), lactate/pyruvate transporter MCT1, and CD147, purported chaperone protein for MCT1 rat-derived L6 skeletal muscle cell line rat plantaris muscle. As well, interaction related proteins was confirmed by Western blotting mitochondrial fractions...