A5032846362- 14-3-3 protein interactions
- interferon and immune responses
- Heme Oxygenase-1 and Carbon Monoxide
- Galectins and Cancer Biology
- Hemoglobin structure and function
- Autophagy in Disease and Therapy
- Monoclonal and Polyclonal Antibodies Research
- Nitric Oxide and Endothelin Effects
- Redox biology and oxidative stress
- Cytokine Signaling Pathways and Interactions
- Proteoglycans and glycosaminoglycans research
- Viral Infections and Vectors
- Ubiquitin and proteasome pathways
- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Inflammasome and immune disorders
- RNA regulation and disease
- Vasculitis and related conditions
- SARS-CoV-2 and COVID-19 Research
- NF-κB Signaling Pathways
- Pulmonary Hypertension Research and Treatments
- Phytochemistry and Bioactive Compounds
- Mosquito-borne diseases and control
- HIV Research and Treatment
- Macrophage Migration Inhibitory Factor
University of Toledo
2016-2024
Ohio Department of Health
2023
Environmental Protection Agency
2023
Battelle
2023
E Ink (South Korea)
2023
Taylor University
2023
The Ohio State University
2023
Bowling Green State University
2023
Kent State University
2023
University of Akron
2023
Heme proteins play essential roles in biology, but little is known about heme transport inside mammalian cells or how inserted into soluble proteins. We recently found that nitric oxide (NO) blocks from inserting several proteins, including cytochrome P450s, hemoglobin, NO synthases, and catalase. This finding led us to explore the basis for inhibition identify cytosolic may be involved, using inducible synthase (iNOS) as a model target. Surprisingly, we GAPDH plays key role. was associated...
The interferon (IFN) system represents the first line of defense against a wide range viruses. Virus infection rapidly triggers transcriptional induction IFN-β and IFN Stimulated Genes (ISGs), whose protein products act as viral restriction factors by interfering with specific stages virus life cycle, such entry, transcription, translation, genome replication, assembly egress. Here, we report new mode action an ISG, IFN-induced TDRD7 (tudor domain containing 7) inhibited paramyxovirus...
Interferon (IFN) regulatory factor 3 (IRF3) is a transcription activated by phosphorylation in the cytoplasm of virus-infected cell; translocating to nucleus, it induces IFN-β and other antiviral genes. We have previously reported IRF3 can also be activated, as proapoptotic factor, its linear polyubiquitination mediated RIG-I pathway. Both transcriptional apoptotic functions contribute effect. Here, we report nontranscriptional function IRF3, namely, repression IRF3-mediated NF-κB activity...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a glycolytic enzyme that also functions in transcriptional regulation, oxidative stress, vesicular trafficking, and apoptosis. Because GAPDH required for the insertion of cellular heme into inducible nitric oxide synthase [Chakravarti, R., et al. (2010) Proc. Natl. Acad. Sci. U.S.A. 107, 18004–18009], we extensively characterized binding properties GAPDH. Substoichiometric amounts ferric bound to (one per tetramer) form low-spin complex...
Virus-infected cells trigger a robust innate immune response and facilitate virus replication. Here, we review the role of autophagy in infection, focusing on both pro-viral anti-viral host responses using select group viruses. Autophagy is cellular degradation pathway operated at basal level to maintain homeostasis induced by external stimuli for specific functions. The degradative function considered response. However, double-edged sword viral infection; viruses often benefit from it,...
Interferon (IFN) regulatory factors (IRF) are key transcription in cellular antiviral responses. IRF7, a virus-inducible IRF, expressed primarily myeloid cells, is required for transcriptional induction of IFNα and genes. IRF7 activated by virus-induced phosphorylation the cytoplasm, leading to its translocation nucleus activity. Here, we revealed non-transcriptional activity contributing functions. interacted with pro-inflammatory factor NF-κB-p65 inhibited inflammatory target Using...
The syndecans are the major family of transmembrane proteoglycans in animals and known for multiple roles cell interactions growth factor signalling during development, inflammatory response, wound-repair tumorigenesis. Although have been cloned from several invertebrate vertebrate species, extent conservation across animal kingdom is unknown there gaps our knowledge chordate syndecans. Here, we develop a new level whole syndecan family, by combining molecular phylogeny protein sequences...
The evolutionarily conserved kelch-repeat protein muskelin was identified as an intracellular mediator of cell spreading. We discovered that its morphological activity is controlled by association with RanBP9/RanBPM, a involved in transmembrane signaling and complex. By subcellular fractionation, endogenous present both the nucleus cytosol. Muskelin localization coregulated C terminus, which provides cytoplasmic restraint also controls interaction RanBP9, atypical lissencephaly-1 homology...
Idiopathic pulmonary arterial hypertension (IPAH) is a rare and progressive disease. Several processes are believed to lead the fatal narrowing seen in IPAH including vasoconstriction, cellular proliferation inflammation, vascular remodeling, abnormalities lung matrix, situ thrombosis. Nitric oxide (NO) produced by NO synthases (NOS) potent vasodilator plays important roles many other platelet function. Reduced levels patients with known contribute development of its complications. Platelet...
Large vessel vasculitides (LVV) are a group of autoimmune diseases characterized by injury to and anatomic modifications large vessels, including the aorta its branch vessels. Disease etiology is unknown. This study was undertaken identify antigen targets within affected walls in aortic root, ascending aorta, arch surgical specimens from patients with LVV, giant cell arteritis, Takayasu isolated focal aortitis. Thoracic aneurysm autologous blood were acquired consenting who underwent...
Cell protrusions contribute to cell motility and migration by mediating the outward extension initial adhesion of edges. In many cells, these extensions are supported actin bundles assembled cross-linking protein, fascin. Multiple extracellular cues regulate fascin here we focus on mechanism which transmembrane proteoglycan, syndecan-1, specifically activates lamellipodial spreading fascin-and-actin bundling when clustered either thrombospondin-1, laminin, or antibody syndecan-1 domain....
Significance Inflammatory arthritis (IA) affects more than 1% of the world population. Natural host-protective immune responses to suppress or prophylactic immunization prevent IA remain unknown. We previously showed that 14-3-3ζ is an autoantigen in large vessel vasculitis. In current study, we examined role antigenic using animal models IA. Our results endogenous suppressor addition immunological manipulations, has a strong effect on bone remodeling. Moreover, developed 14-3-3ζ–based...
Immunosuppressed kidney transplant (KT) recipients produce a weaker response to COVID-19 vaccination than immunocompetent individuals. We tested antiviral IgG in 99 KT and 66 healthy volunteers who were vaccinated with mRNA-1273 Moderna or BNT162b2 Pfizer-BioNTech vaccines. A subgroup of participants had their peripheral blood leukocytes (PBLs) evaluated for the frequency T helper 1 (Th1) cells producing IL-2, IFN-γ and/or TNF-α, IL-10-producing T-regulatory (Tr) cells. Among recipients,...
The interferon system is the first line of defense against virus infection. Recently, using a high-throughput genetic screen human interferon-stimulated gene short-hairpin RNA library, we identified viral restriction factor, TDRD7 (Tudor domain-containing 7). inhibits paramyxo-/pneumoviruses (e.g. Sendai and respiratory syncytial virus) by interfering with virus-induced cellular autophagy pathway, which these viruses use for their replication. Here, report that factor herpes simplex (HSV-1)....
Significance Blocking IL-17A signaling is an attractive therapy in treating autoimmune diseases. Current blockers target both branches, leading to inhibition of TRAF6-based IL-6 production, and TRAF5-based CXCL-1 production. Global suppression branches concern due the critical role plays host-defense mechanisms. Our study shows that 14-3-3ζ necessary for IL-17A–stimulated We also found represses TRAF5-mediated induction. Taken together, our results show a unique cross-talk between two via...