A5074395094- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Botulinum Toxin and Related Neurological Disorders
- Cardiac electrophysiology and arrhythmias
- Pharmacology and Obesity Treatment
- Traditional Chinese Medicine Analysis
- Chromatography in Natural Products
- Biochemical effects in animals
- Pharmacological Effects of Natural Compounds
- Medicinal Plant Pharmacodynamics Research
- Ion Transport and Channel Regulation
- Neuroscience and Neural Engineering
- Pharmacological Receptor Mechanisms and Effects
- Nicotinic Acetylcholine Receptors Study
- Neuropeptides and Animal Physiology
- Circadian rhythm and melatonin
- Adenosine and Purinergic Signaling
- RNA regulation and disease
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Stress Responses and Cortisol
- Nerve injury and regeneration
- Glycogen Storage Diseases and Myoclonus
- Connexins and lens biology
Center for Research and Advanced Studies of the National Polytechnic Institute
2014-2024
Instituto Politécnico Nacional
2022
Centro de Investigación en Materiales Avanzados
2008-2012
University of Virginia
2002-2008
University of Virginia Health System
2004
Background potassium channels determine membrane potential and input resistance serve as prominent effectors for modulatory regulation of cellular excitability. TREK-1 is a two-pore domain background K+ channel (KCNK2, K2P2.1) that sensitive to variety physicochemical humoral factors. In this work, we used recombinant expression system show activation G alpha(q)-coupled receptors leads inhibition via protein kinase C (PKC), identified critical phosphorylation site in key regulatory mediates...
Background: The participation of spinal P2X receptors in neuropathic pain is well recognized. However, the role P2Y has been less studied. purpose this study was to investigate contribution 6,11 following peripheral nerve damage induced by ligation. In addition, we determined expression dorsal cord presence selective antagonists. Furthermore, evaluated microglia and astrocytes pronociceptive receptors. Results: Spinal administration 6 (MRS2578, 10–100 μM) 11 (NF340, 0.3–30 receptor...
Abstract Ether-à-go-go-1 (Eag1) potassium channels are potential tools for detection and therapy of numerous cancers. Here, we show human Eag1 (hEag1) regulation by cancer-associated factors. We studied hEag1 gene expression its estradiol, antiestrogens, papillomavirus (HPV) oncogenes (E6/E7). Primary cultures from normal placentas cervical cancer tissues; tumor cell lines cervix, choriocarcinoma, keratinocytes, lung; vascular endothelium, lung were used. Reverse transcription-PCR (RT-PCR)...
Extrasynaptic α5 -subunit containing GABAA (α5 -GABAA ) receptors participate in chronic pain. Previously, we reported a sex difference the action of dysfunctional However, underlying mechanisms remain unknown. The aim this study was to examine sexual dimorphism neuropathic rodents and involved. Female male Wistar rats or ICR mice were subjected nerve injury followed by receptor inverse agonist intrathecal administration, L-655,708. drug produced an antiallodynic effect nerve-injured female...
Reticular thalamocortical neurons express a slowly inactivating T-type Ca(2+) current that is quite similar to recorded from recombinant Ca(v)3.3b (alpha1Ib) channels. These also abundant Ca(v)3.3 mRNA, suggesting it underlies the native current. Here, we test this hypothesis by comparing anesthetic sensitivities of channels stably expressed in HEK 293 cells T reticular thalamic (nRT) brain slices young rats. Barbiturates completely blocked both and nRT currents, with pentobarbital being...
High voltage‐activated Ca 2+ channel expression and gating is controlled by their β subunits. Although the sites of interaction are known at atomic level, how modulates remains to be determined. Using a chimeric approach, subunit regulation was conferred low channel. Regulation dependent on rigid linker connecting α 1 domain IS6. Chimeric channels also revealed role for IS6 in gating. Taken together, these results support direct coupling model where subunits alter movements that occur as...
Molecular diversity in T-type Ca(2+) channels is produced by expression of three genes, and alternative splicing those genes. Prompted differences noted between rat human Ca(v)3.3 sequences, we searched for splice variants. We cloned six variants, which are at exon 33 34. Expression the variants differed brain regions. The electrophysiological properties displayed similar voltage-dependent gating, but their kinetic properties. functional impact was inter-related, suggesting an interaction....
Low-voltage-activated T-type (Cav3) Ca2+ channels produce low-threshold spikes that trigger burst firing in many neurons. The CACNA1I gene encodes the Cav3.3 isoform, which activates and inactivates much more slowly than other Cav3 channels. These distinctive kinetic features, along with its brain-region-specific expression, suggest endow neurons ability to generate long-lasting bursts of firing. human contains two regions alternative splicing: variable inclusion exon 9 an acceptor site...
Bestrophin-1 and anoctamin-1 are members of the calcium-activated chloride channels (CaCCs) family involved in inflammatory neuropathic pain. However, their role pain hypersensitivity induced by REM sleep deprivation (REMSD) has not been studied. This study aimed to determine if bestrophin-1 REMSD. We used multiple-platform method induce for 48 h tactile allodynia a transient increase corticosterone concentration at beginning protocol (12 h) female male rats. REMSD enhanced c-Fos α2δ-1...
The Ca(v)beta subunits of high voltage-activated Ca(2+) channels control the trafficking and biophysical properties alpha(1) subunit. Ca(v)beta-alpha(1) interaction site has been mapped by crystallographic studies. Nevertheless, how this leads to channel regulation not determined. One hypothesis is that betas regulate gating modulating movements IS6. A key requirement for direct-coupling model linker connecting IS6 alpha-interaction domain (AID) be a rigid structure.The present study tests...
Transient receptor potential ankyrin 1 (TRPA1) is a non-selective cation channel expressed by subset of nociceptive neurons that acts as multimodal receptor. Its activity contributes to modulate transmission in acute inflammatory pain. However, the role this chronic pain has been less studied. The purpose study was investigate local peripheral and spinal participation TRPA1 channels formalin-induced long-lasting hypersensitivity.Formalin (1%)-induced hypersensitivity determined application...
PKC and PKA phosphorylation inhibit TREK-1 channels downstream of Gs protein-coupled receptor activation in vitro. However, the role neuropathic pain is unknown. The purpose this study was to investigate whether altered channel function by modulators contributes antiallodynia rats. Furthermore, we investigated if vitro described sites for (S300 S333, respectively) participate modulation naïve L5/L6 spinal nerve ligation (SNL) induced tactile allodynia. Intrathecal injection BL-1249 (TREK-1...