A5101679998- Helicobacter pylori-related gastroenterology studies
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Galectins and Cancer Biology
- Biochemical and Molecular Research
- Clostridium difficile and Clostridium perfringens research
- Nutritional Studies and Diet
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- RNA modifications and cancer
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Genetics, Aging, and Longevity in Model Organisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Infrared Thermography in Medicine
- Metabolism, Diabetes, and Cancer
- Medical and Biological Sciences
- Liver Disease Diagnosis and Treatment
- Renal and related cancers
- Caveolin-1 and cellular processes
- Protist diversity and phylogeny
- Escherichia coli research studies
- Endoplasmic Reticulum Stress and Disease
The University of Texas Southwestern Medical Center
2019-2023
Southwestern Medical Center
2023
Harbin Medical University
2018-2021
Shanghai University of Medicine and Health Sciences
2021
Chinese Academy of Sciences
2014-2017
Fujian Institute of Research on the Structure of Matter
2015-2017
Huazhong Agricultural University
2012
Abstract The sperm calcium channel CatSper plays a central role in successful fertilization as primary Ca 2+ gateway. Here, we applied cryo-electron tomography to visualize the higher-order organization of native complex intact mammalian sperm. repeating units form long zigzag-rows along mouse and human flagella. Above each tetrameric pore, most extracellular domains canopy that interconnects zigzag-shaped roof. Murine contains an additional wing-structure connected channel. intracellular...
Abstract GRAS proteins belong to a plant-specific protein family with many members and play essential roles in plant growth development, functioning primarily transcriptional regulation. Proteins the are minimally defined as containing conserved domain. Here, we determined structure of domain Os-SCL7 from rice (Oryza sativa) 1.82 Å. The includes cap core subdomains elucidates features LRI, VHIID, LRII, PFYRE, SAW motifs. is dimer, clear groove accommodate double-stranded DNA. Docking DNA...
The transport of the CagA effector into gastric epithelial cells by Cag Type IV secretion system (Cag T4SS) Helicobacter pylori (H. pylori) is critical for pathogenesis. recruited to T4SS Cagβ ATPase. CagZ, a unique protein in H. pylori, regulates Cagβ-mediated transport, but underlying mechanisms remain unclear. Here we report crystal structure cytosolic region Cagβ, showing typical ring-like hexameric assembly. central channel ring narrow, suggesting that must unfold through channel. Our...
Cilia and flagella are evolutionarily conserved eukaryotic organelles involved in cell motility signaling. In humans, mutations Radial Spoke Head Component 4A (
White spot syndrome virus (WSSV) is one of the major and most serious pathogen in shrimp industry. As abundant envelope protein, VP24 acts as a core protein interacting with other structure proteins plays an important role assembly infection. Here, we have presented crystal from WSSV. In structure, consists nine-stranded β-barrel fold mostly antiparallel β-strands, loops extending out at both N-terminus C-terminus, which distinct to those two VP28 VP26. Structural comparison VP26 reveals...
The VirB/D type IV secretion system (T4SS) plays an essential role in materials transport between host cells and pathogenic Helicobacter pylori is considered the major mediator of H. pylori-associated gastric disease. VirB8, inner membrane protein that interacts with many other proteins, a crucial component for secretory function. Here, we present crystal structure periplasmic domain CagV, VirB8 counterpart Cag-T4SS. reveals fold similar to members except absence α5 helix, discontinuous β1...
Helicobacter pylori , a Gram-negative bacterial pathogen prevalent in the human population, is causative agent of severe gastric diseases. An H. type IV secretion (T4S) system encoded by cytotoxin-associated gene pathogenicity island ( cag PAI) responsible for communication with host cells. As component PAI T4S core complex, CagX plays an important role virulence-protein translocation into In this work, crystal structure C-terminal domain (CagXct), which homologue VirB9 protein from VirB/D4...
Motile cilia have a '9+2' structure containing nine doublet microtubules and central apparatus (CA) composed of two singlet with associated projections. The CA plays crucial roles in regulating ciliary motility. Defects assembly or function usually result motility-impaired paralyzed cilia, which humans causes disease. Despite their importance, the protein composition functions most projections remain largely unknown. Here, we combined genetic, proteomic cryo-electron tomographic approaches...
This study aimed to elucidate whether dietary amino acids (AAs) composition is associated with type 2 diabetes mellitus (T2DM) and investigate how serum AAs profiles mediated this association. Two prospective cohorts of 1750 4024 adults were enrolled. Dietary compositions index (AACI) was developed reflect the overall quality composition. Multivariate linear regression logistic models used examine associations AACI T2DM. The incidence T2DM relative risk 95%CI from bottom top tertiles being...
Helicobacter pylori is a well-known pathogen involved in the development of peptic ulcer, gastric adenocarcinoma and other forms cancer. Recently, there has been more considerable interest strain-specific genes located plasticity regions with great genetic variability. However, little known about many these genes. Studies suggested that certain this region may play key roles pathogenesis H. pylori-associated gastroduodenal diseases. JHP933, conserved putative protein unknown function,...
Abstract The sperm calcium channel CatSper plays a central role in successful fertilization as primary Ca 2+ gateway into the flagellum. However, CatSper’s complex subunit composition has impeded its reconstitution vitro and structural elucidation. Here, we applied cryo-electron tomography to visualize macromolecular organization of native intact mammalian sperm, well identified three additional CatSper-associated proteins. repeating units form long zigzag-rows four nanodomains along...
Abstract ‘9+2’ motile cilia contain 9 doublet microtubules and a central apparatus (CA) composed of two singlet with associated projections. The CA plays crucial roles in regulating ciliary motility. Defects assembly or function usually result motility-impaired paralyzed cilia, which humans causes disease. Despite their importance, the protein composition functions most projections remain largely unknown. Here, we combined genetic approaches quantitative proteomics cryo-electron tomography...
Human DAB2 interaction protein (DAB2IP) is a member of Ras-GTPase activating family and functions as tumor suppressor, implying it could serve prognostic biomarker in cancers. Here we generated mouse monoclonal antibody, 2A4, directed against human DAB2IP. This antibody was identified IgG1 specifically recognizes DAB2IP both its native denatured forms. It will useful versatile tool for further mechanistic study development the potential significance
Helicobacter pylori is a well-known bacterial pathogen involved in the development of peptic ulcer, gastric adenocarcinoma and other forms cancer. Evidence has suggested that certain strain-specific genes plasticity region may play key roles pathogenesis H. pylori-associated gastroduodenal diseases. Therefore there considerable interest located regions pylori. JHP933 encoded by gene strain J99. Recently, crystal structure confirmed it as nucleotidyltransferase (NTase) superfamily protein...
Since its discovery a decade ago, microRNA has been identified as one of the major regulatory gene families in eukaryotic cells. Many functions microRNAs have revealed both flora and fauna recent years, but transcriptional regulation genes is not well-understood. In present study, series primers were designed 2000 nt upstream regions porcine miR-181 miR-1 then sequences cloned into pGL3-basic vector to test their activity. Dual-luciferase reporter assays showed that, activity 5′-flanking...
GRAS proteins, named after the first three members GAI, RGA and SRC, has been found in 294 embryophyta species is represented by 1,035 sequences. They belong to a plant-specific protein family play essential roles plant growth development. Proteins this are defined as minimally containing conserved domain, which about 350-450 resides can be subdivided into five distinct motifs with their name derived from most prominent amino acids: LRI (leucine-rich region I), VHIID, LRII II), PFYRE SAW...
Abstract Background: This study aimed to elucidate whether dietary amino acids (AAs) composition is associated with type 2 diabetes mellitus (T2DM), and investigate how serum AAs profiles mediated this association.Methods: Two prospective cohorts of 1750 4024 adults aged 20-74 year-old were enrolled mean values 4.2 5.3 years follow-up. Dietary compositions index (AACI) was developed reflect the overall quality lower AACI value, higher compositions. Multivariate linear regression logistic...
Abstract Background: Typical pseudogenes expressions have been reported to associated with liver diseases. However, whether contribute non-alcoholic fatty disease (NAFLD) and triglyceride (TG) accumulation the underlying molecular mechanisms are poorly understood. We aimed elucidate role of Gm12664-201 in TG potential mechanism. Method Results: identified Gm12664-01 high-fat diet (HFD) fed mice using microarray confirmed its expression both vivo vitro high fat models quantitative Real...