A5037648785- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Lung Cancer Treatments and Mutations
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Acute Lymphoblastic Leukemia research
- Glioma Diagnosis and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- RNA modifications and cancer
- Chronic Myeloid Leukemia Treatments
- Brain Metastases and Treatment
- Single-cell and spatial transcriptomics
- Multiple Myeloma Research and Treatments
- Platelet Disorders and Treatments
- Blood groups and transfusion
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Lung Cancer Research Studies
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
First Affiliated Hospital of Zhengzhou University
2013-2025
China Medical University
2021-2025
First Hospital of China Medical University
2021-2025
Shenzhen Bioeasy Biotechnology (China)
2020-2024
Microbiology Institute of Shaanxi
2024
Xi'an Botanical Garden of Shaanxi Province
2024
Mellanox Technologies (Israel)
2021-2023
Sun Yat-sen University
2015-2022
Yanshan University
2009-2022
State Key Laboratory of Metastable Materials Science and Technology
2022
Glioblastoma (GBM) is the most common primary brain cancer in adults and notoriously difficult to treat because of its diffuse nature. We performed single-cell RNA sequencing (RNA-seq) on 3,589 cells a cohort four patients. obtained from tumor core as well surrounding peripheral tissue. Our analysis revealed cellular variation tumor's genome transcriptome. were also able identify infiltrating neoplastic regions lesions. Despite existence significant heterogeneity among cells, we found that...
Knowledge of immune cell phenotypes, function, and developmental trajectory in acute myeloid leukemia (AML) microenvironment is essential for understanding mechanisms evading surveillance immunotherapy response targeting special components.Using a single-cell RNA sequencing (scRNA-seq) dataset, we analyzed the bone marrow (BM) samples from 16 AML patients 4 healthy donors, but not blasts.We observed significant difference between normal BM cells. Here, defined diversity dendritic cells (DC)...
This communication reports a DNA tetrahedron whose self-assembly is triggered by an acidic environment. The key element the formation/dissociation of short, cytosine (C)-containing, triplex. As solution pH value oscillates between 5.0 and 8.0, triplex will form dissociate that, in turn, leads to assembly or disassembly tetrahedron, which has been demonstrated native polyacrylamide gel electrophoresis (PAGE). We believe that such environment-responsive behavior be important for potential...
In this study, we retrospectively summarized the differences of molecular gene mutations between MDS and AML patients, as well young older age groups patients. We also analyzed response newly diagnosed patients to standard DA or IA induction chemotherapy relationship outcome frequency different mutation abnormalities.NGS assay covering 43 genes was studied in 93 de novo 325 non-M3 Bone marrow samples from all underwent mutational analysis by NGS.At least one non-synonymous detected 279...
Cellular immunotherapy represented by CD19-directed chimeric antigen receptor T (CAR-T) cells has achieved great success in recent years. An increasing number of CAR-T therapies are being developed for cancer treatment, but the frequent and varied adverse events, such as "on-target, off-tumor toxicity", limit application. Here, we identify target expression patterns CAR 18 tissues organs (peripheral blood mononuclear cells, bone marrow, lymph nodes, spleen, heart, ascending aortic tissue,...
Using the stopped-flow circular dichroism (SFCD) technique, we investigate kinetics of pH-induced folding and unfolding process DNA i-motif. The results show that molecule can fold or unfold on a time scale 100 ms when solution pH is changed. It also found rates strongly depend pH. On basis quantitative data, propose theoretical models to decipher kinetics. Our suggest cooperativity protons crucial for both process. In process, cooperative neutralization two (out total six in i-motif...
In this single-arm phase II study (NCT03557099), we evaluated the efficacy and safety of hetrombopag, a small molecule thrombopoietin (TPO) receptor agonist, in patients with severe aplastic anemia (SAA) who were refractory to standard first-line immunosuppressive therapy (IST).SAA IST given hetrombopag orally at an initial dose 7.5 mg once daily maximum 15 daily, for total 52 weeks. The primary endpoint was proportion achieving hematologic responses ⩾1 lineage week 18.A 55 eligible enrolled...
Abstract Immune thrombocytopenia (ITP) is an autoimmune disorder characterized by reduced platelet levels and heightened susceptibility to bleeding resulting from augmented autologous destruction diminished thrombopoiesis. Although antibody‐mediated reactions are widely recognized as primary factors, the precise etiological agents that trigger ITP remain unidentified. The pathogenesis of remains unclear owing absence comprehensive high‐throughput data, except for belated emergence...
Abstract This study was designed to explore whether exosomal sphingosine 1‐phosphate (S1P) from mesenchymal stem cells (MSCs) regulate the Treg/Th17 balance in aplastic anemia (AA) patients and validate underlying mechanism. To address this, exosomes human bone marrow MSCs (MSCs‐Exos) were co‐cultured with CD4 + T AA (AA cells), which transfected si‐S1PR1, si‐S1PR3, or not. The proportion of Th17 Treg evaluated by flow cytometry. levels Th17‐associated interleukin‐17 (IL‐17), Treg‐associated...
Abstract Anemia is a significant complication of chronic inflammation and may be related to dysregulated activities among erythroblastic island (EBI) macrophages. GM-CSF was reported upregulated attracted as therapeutic target in many inflammatory diseases. Among EBIs, we found that the receptor preferentially highly expressed EBI macrophages but not erythroblasts. treatment significantly decreases human formation vitro by decreasing adhesion molecule expression CD163. RNA-sequence analysis...
Abstract Aplastic anemia (AA) is a bone marrow (BM) failure syndrome mediated by hyperactivated T‐cells with heterogeneous pathogenic factors. The onset of BM cannot be accurately determined in humans; therefore, exact pathogenesis remains unclear. In this study, cellular atlas and microenvironment interactions established using unbiased single‐cell RNA‐seq, along multi‐omics analyses (mass cytometry, cytokine profiling, oxidized fatty acid metabolomics). A new KIR + CD8 regulatory T cells...