A5068636536- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- Intraperitoneal and Appendiceal Malignancies
- Porphyrin and Phthalocyanine Chemistry
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Aquaculture disease management and microbiota
- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Glycosylation and Glycoproteins Research
- Cellular Mechanics and Interactions
- Lymphatic System and Diseases
- Photoacoustic and Ultrasonic Imaging
- Cancer, Hypoxia, and Metabolism
- Orbital Angular Momentum in Optics
- Microfluidic and Bio-sensing Technologies
- Receptor Mechanisms and Signaling
- Virology and Viral Diseases
- Monoclonal and Polyclonal Antibodies Research
- Ovarian cancer diagnosis and treatment
- Systemic Sclerosis and Related Diseases
- Bladder and Urothelial Cancer Treatments
- Phosphodiesterase function and regulation
Massachusetts General Hospital
2018-2024
Harvard University
2018-2024
Moscow Research and Clinical Center for Neuropsychiatry
2018
The University of Texas at Austin
2010-2016
Abstract A major objective in developing new treatment approaches for lethal tumors is to reduce toxicity normal tissues while maintaining therapeutic efficacy. Photodynamic therapy ( PDT ) provides a mechanistically distinct approach treat without the systemic of chemotherapy drugs. involves light‐based activation small molecule, photosensitizer PS ), generate reactive molecular species RMS that are toxic target tissue. Depending on localization, various cellular and subcellular components...
A key reason for the persistently grim statistics associated with metastatic ovarian cancer is resistance to conventional agents, including platinum-based chemotherapies. major source of treatment failure high degree genetic and molecular heterogeneity, which results from significant underlying genomic instability, as well stromal physical cues in microenvironment. Ovarian commonly disseminates via transcoelomic routes distant sites, frequent production malignant ascites, poorest prognosis....
Abstract Helper and cytotoxic T cells accomplish focused secretion through the movement of vesicles toward microtubule organizing center (MTOC) translocation MTOC to target contact site. In this study, using Jurkat OT-I TCR transgenic primary murine CTLs, we show that dynein-binding proteins nuclear distribution E homolog 1 (NDE1) dynactin (as represented by p150Glued) form mutually exclusive complexes with dynein, exhibit nonoverlapping distributions in target-stimulated cells, mediate...
Photodynamic therapy (PDT) is an established therapeutic modality that uses nonionizing near-infrared light to activate photocytotoxicity of endogenous or exogenous photosensitizers (PSs). An ongoing avenue cancer research involves leveraging PDT stimulate antitumor immune responses; however, these effects appear be best elicited in low-dose regimens do not provide significant tumor reduction using conventional, nonspecific PSs. The loss enhancement at higher doses may arise part from...
Abstract Quantitative PCR (qPCR) was used to investigate whether largemouth bass virus (LMBV) can exist within biofilms. Suspended LMBV partitioned into either laboratory-grown Pseudomonas fluorescens biofilms or pond-grown, mixed-population Biofilm-entrapped retained infectivity when tested on epithelioma papillosum cyprini tissue culture cells. The associated with P. were resistant disinfection by sodium hypochlorite and an iodine-based compound (betadine) but susceptible ethanol....
ABSTRACT In this study, using Jurkat cells, we show that DISC1 (disrupted in schizophrenia 1) and Girdin (girders of actin filament) are essential for typical accumulation at the immunological synapse. Furthermore, DISC1, dynein bound a complex. Although complex initially forms as central patch synapse, it relocates to peripheral ring corresponding supramolecular activation cluster (pSMAC). absence classic does not form, cell spreading is blocked, fails relocate pSMAC. A similar effect seen...
Photodynamic therapy (PDT), is a clinically-approved light-based anti-cancer treatment modality in which photoactivatable photosensitizer irradiated with an appropriate wavelength of light to generate cytotoxic molecules kill cancer cells. In this article, we describe vitro PDT protocol using 3-dimensional (3D) model ovarian that was established on beds Matrigel. performed liposomal formulation verteporfin (Visudyne®). The cells were genetically-labeled the fluorescent protein mCherry...
Abstract Background Advanced epithelial ovarian cancer (EOC) has high recurrence rates due to disseminated initial disease presentation. Cytotoxic phototherapies, such as photodynamic therapy (PDT) and photoimmunotherapy (PIT, cell-targeted PDT), have the potential treat malignancies safe intraperitoneal delivery. Methods We use in vitro measurements of EOC tumour cell T responses chemotherapy, PDT, epidermal growth factor receptor targeted PIT inputs a mathematical model non-linear immune...
Drug resistance to conventional therapies remains a major cause of treatment failure, tumor recurrence and dismal survival rates for patients with advanced stage cancers. Photodynamic therapy (PDT) provides an opportunity exploit photochemically-triggered death mechanisms via targeting sub-cellular, cellular stromal compartments overcome in unresponsive populations stubborn disease. The informed design mechanism-based combinations is emerging as increasingly important improving the efficacy...
Abstract Dramatic cytoskeletal activities and protein movements are seen when T cells engage cognate targets or antigen presenting cells. These include formation of a specialized contact site known as the immunological synapse (IS), clustering vesicles around microtubule organizing center (MTOC) translocation MTOC up to IS. Ultimately, this leads focused secretion towards target. In an effort understand how takes place, we have investigated roles dynein, p150Glued, Lis1,NDE1 NDEL1 using...
Previous studies have demonstrated that flow-induced shear stress induces a motile and aggressive tumor phenotype in microfluidic model of 3D ovarian cancer. However, the magnitude distribution hydrodynamic forces influence this biological modulation on cancer nodules are not known. We developed series numerical experimental tools to identify these within microchannel. In work, we used particle image velocimetry (PIV) find velocity profile using fluorescent micro-spheres as surrogates...
Abstract DISC1 (Disrupted in Schizophrenia-1) is a dynein-binding scaffold protein that interacts with many other proteins. In neurons, important for proliferation, migration, and signaling. We found expressed Jurkat cells human NK it coimmunoprecipitates dynein cells. Immunostaining unstimulated showed was generally located around the MTOC. When were stimulated SEE-coated Raji cells, depending on antibody used, we either clustered mitochondria or colocalized NDE1 at immunological synapse...
Exploiting differences in photosensitizer (PS) localization and mechanisms of action with sequential or simultaneous activation protocols has been shown to improve photodynamic therapy (PDT) efficacy. Various sub-cellular, cellular stromal components can be targeted, causing selective photodamage. Previous reports have that rationally targeting non-overlapping tumor compartments sub-cellular sites considerably enhances outcomes from PDT. The current presentation describes the benefits...
A range of cellular, architectural, and physical cues in the tumor microenvironment influence intrinsic acquired resistance mechanisms that lead to treatment failure. Strategies leverage photodynamic therapy (PDT), a photochemistry-based biophysical modality, regionally target prime stubborn populations may be essential realizing durable improvements cancer management while minimizing toxicity from traditional agents. Capturing these attributes rationally-designed combinations has shown...