A5070806524- Asthma and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Enzyme function and inhibition
- Respiratory and Cough-Related Research
- Peptidase Inhibition and Analysis
- Inhalation and Respiratory Drug Delivery
- Synthesis and Catalytic Reactions
- Enzyme Structure and Function
- COVID-19 Clinical Research Studies
- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Chemical Reactions and Mechanisms
- Eosinophilic Esophagitis
- Gallbladder and Bile Duct Disorders
- Bacteriophages and microbial interactions
- Health, psychology, and well-being
- Enzyme Production and Characterization
- Protein Structure and Dynamics
- Glycosylation and Glycoproteins Research
- Allergic Rhinitis and Sensitization
- Long-Term Effects of COVID-19
- Medication Adherence and Compliance
- Fluorine in Organic Chemistry
- Lung Cancer Treatments and Mutations
- IL-33, ST2, and ILC Pathways
Copenhagen University Hospital
2019-2025
Bispebjerg Hospital
2024-2025
Hvidovre Hospital
2019-2024
University of Copenhagen
1991-2020
Zealand University Hospital Køge
2020
Amager Hospital
2019
Leiden University Medical Center
2018
University of Illinois Urbana-Champaign
1999-2002
Cargotec (Poland)
2000-2001
University of Oxford
1998
BackgroundThe development of novel targeted biologic therapies for severe asthma has provided an opportunity to consider remission as a new treatment goal.Research QuestionHow many patients with treated therapy achieve clinical remission, and what predicts response treatment?Study Design MethodsThe Danish Severe Asthma Register is nationwide cohort including all adult receiving in Denmark. This observational study defined "clinical response" following 12 months ≥ 50% reduction exacerbations...
Background Biologics can induce remission in some patients with severe asthma, however, little is known about pre-biologic disease trajectories and their association outcomes from biological treatment. We aimed to identify long-term of progression initiating biologics investigate trajectory associations burden impact on biologic therapy efficacy. Methods Patients the Danish Severe Asthma Registry between 2016–2022 were included followed retrospectively prescription databases starting 1995....
CONTENTS – Sulfonamide inhibitors Metal coordination in carbonic anhydrase Binding of anions Regular tetra‐coordination Distorted Penta‐coordination Uncoordinated Mutations the doorkeeper function Substrate binding The catalytic mechanism
Combining the antibiotic azithromycin and hydroxychloroquine induces airway immunomodulatory effects, with latter also having
Introduction As a common chronic disease seen across all ages, asthma has the potential to incur high societal and individual costs from both direct healthcare loss of productivity. Most previous studies use smaller, selected populations assess cost asthma, possibly reducing generalisability. We, therefore, aimed total, nationwide economic burden by severity an perspective. Methods The annual was assessed in Danish cohort patients aged 18–45 during 2014–2016 as excess costs, income welfare...
Severe asthma impacts work capacity, but little is known about the effects of biologic therapy on patients' ability to work. We aimed assess impact for severe capacity and workforce attachment. This cohort study used Danish Asthma Register, comprising all patients with initiating therapy. Earned income, hours worked, attachment were tracked in national databases from one year prior as well during 2 years Outcomes compared age-, sex-, cohabitation- residence-matched controls general...
Abstract Background In severe asthma, intensive (“ supratherapeutic ”) doses of inhaled corticosteroids (ICS) are often used. The prevalence ICS use and its impact on corticosteroid-related comorbidities is poorly understood. We aimed to describe the in relation comorbidities, changes prescribed redeemed dose after 12 months biologic therapy. Methods Patients from nationwide Danish Severe Asthma Register (DSAR) receiving therapy > were included. Supratherapeutic defined as 1600 µg...
The three‐dimensional structure of human carbonic anhydrase II complexed with azide and bromide was investigated crystallographically. Both these non‐protonated inhibitors replace the zinc ‘deep’ water, two catalytically important water molecules in active site molecule. ions bind a distorted tetrahedral manner 0.4 1.1 Å from position, respectively, but are close contact (2.0 2.6 Å, respectively) ion.
Background Although socioeconomic impact on asthma control has been investigated, little is known about its relationship to specialist referral of patients with possible severe asthma, especially in a public healthcare setting. The present study aims identify patterns disease and nationwide cohort adult treated inhaled corticosteroids (ICS). Methods Asthma fulfilling the following criteria were included: aged 18–45 years redeeming two or more prescriptions ICS during 2014–2018 based data...
Phase III regulatory trials show that anti-interleukin (IL)-5 biologics efficiently reduce exacerbations and the use of maintenance oral corticosteroids (mOCS) in patients with severe eosinophilic asthma. However, eligible for these differ significantly compared real-life asthma populations. Therefore, our aim was to explore efficacy a setting. The Danish Severe Asthma Register (DSAR) is complete, nationwide register comprises all on biological therapy asthma.This prospective study...
Abstract Background Asthma is a common disease in childhood and adolescence with lifelong consequences particularly among those at risk of severe disease, poor control and/or frequent exacerbations. Specialist care recommended for at-risk children adolescents, yet access to specialist management free-to-access healthcare settings remains poorly understood. Methods A Danish nationwide cohort adolescents aged 2–17 years persistent asthma, defined as repeated redemption inhaled corticosteroids...
Metal-substituted crystals of human carbonic anhydrase II belonging to space group P21 with cell dimensions a = 42.7, b 41.7, c 73.0 Å and β 104.6° were analyzed crystallographically. The resolution limit ranged from 1.82 1.92 high completeness (86.2–90.7%). Cobalt(II)-substituted has tetrahedral coordination around the metal both at pH 6 7.8, similar native zinc enzyme. In contrast, catalytically inactive copper(II), nickel(II) manganese(II) derivatives showed increased number ion. Whereas...
Purpose: The diagnostic value of fast pulse sequences in MR imaging was compared with US patients clinically suspected acute cholecystitis. Material and Methods: In a prospective study 94 patients, 35 were examined both within 24 h. Results: diagnoses cholecystitis 23, gallbladder common bile duct stones 3, other pathologic conditions the abdomen 7 normal 2 patients. 17, 8, 2, 5 non-conclusive 3 Conclusion: has higher sensitivity than for diagnosing and, increased accessibility, may be first method.
The structures of human carbonic‐anhydrase‐II complexes with the anionic inhibitors hydrogen sulphide (HS − ) and nitrate (NO 3 have been determined by X‐ray diffraction at 0.19‐nm resolution from crystals soaked pH 7.8 6.0, respectively. modes binding these two anions differ markedly each other. strong inhibitor HS replaces native zinc‐bound water/hydroxide (Wat263) leaving tetrahedral metal geometry unaltered acts as a hydrogen‐bonding donor towards Thr199γ. weak NO does not displace...
Paramecium bursaria Chlorella virus PBCV-1 mRNA guanylyl transferase (capping enzyme) has been complexed with an cap analogue G[5′]ppp[5′]G and crystallized. The crystals belong to space group C222 1 unit cell dimensions a = 78.4 Å, b 164.1 c 103.3 diffraction data 3.1 Å collected by using synchrotron radiation. structure solved molecular replacement each of the two domains in previously determined enzyme complex GTP. conformation is open respect active site cleft, all contacts between...
We have shown that 14q32 microRNAs are highly involved in vascular remodelling and cardiovascular disease. However, the locus also encodes 41 'orphan' small nucleolar RNAs (snoRNAs). aimed to gather evidence for an independent role snoRNAs human disease.We performed a lookup of region within dataset genome wide association scan 5244 participants PROspective Study Pravastatin Elderly at Risk (PROSPER). Single nucleotide polymorphisms (SNPs) snoRNA-cluster were significantly associated with...
The crystal structures of three highly potent and selective low-molecular weight rigid peptidyl aldehyde inhibitors complexed with thrombin have been determined refined to R values 0.152−0.170 at 1.8−2.1 Å resolution. Since the selectivity two was >1600 respect trypsin, trypsin-inhibited complexes these were also (R = 0.142−0.157 1.9−2.1 resolution). appears reside in inability a benzenesulfonamide group bind equivalent D-enantiomorphic S3 site thrombin, which may be related lack...
The structure of peptidase T, or tripeptidase, was determined by multiple wavelength anomalous dispersion (MAD) methodology and refined to 2.4 Å resolution. Peptidase T comprises two domains; a catalytic domain with an active site containing metal ions, smaller formed through long insertion into the domain. presumably zinc, are separated 3.3 Å, coordinated five carboxylate histidine ligands. molecular surface is negatively charged. has same basic fold as carboxypeptidase G2. When structures...