A5101692795- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Heat shock proteins research
- Enzyme Structure and Function
- Allelopathy and phytotoxic interactions
- Autophagy in Disease and Therapy
- Single-cell and spatial transcriptomics
- Adipose Tissue and Metabolism
- Telomeres, Telomerase, and Senescence
- Pluripotent Stem Cells Research
- Chemical synthesis and alkaloids
- Neural dynamics and brain function
- Inflammasome and immune disorders
- Hydrocarbon exploration and reservoir analysis
- Plant Pathogens and Fungal Diseases
- Histone Deacetylase Inhibitors Research
- Toxin Mechanisms and Immunotoxins
- Bioactive Natural Diterpenoids Research
- Dietary Effects on Health
- Genomics and Chromatin Dynamics
- Biological and pharmacological studies of plants
- Extracellular vesicles in disease
- Hearing, Cochlea, Tinnitus, Genetics
- Retinal Development and Disorders
- Ubiquitin and proteasome pathways
Institute of Zoology
2018-2023
Chinese Academy of Sciences
2006-2023
Shanghai Institute of Materia Medica
2020-2023
University of Chinese Academy of Sciences
2019-2023
Tsinghua University
2018
East China Normal University
2014
Institute of Grassland Research
2008-2013
Abstract Zinc finger protein with KRAB and SCAN domains 3 (ZKSCAN3) has long been known as a master transcriptional repressor of autophagy. Here, we identify novel role for ZKSCAN3 in alleviating senescence that is independent its autophagy-related activity. Downregulation observed aged human mesenchymal stem cells (hMSCs) depletion accelerates these cells. Mechanistically, maintains heterochromatin stability via interaction heterochromatin-associated proteins nuclear lamina proteins....
Loss of organelle homeostasis is a hallmark aging. However, it remains elusive how this occurs at gene expression level. Here, we report that human mesenchymal stem cell (hMSC) aging associated with dysfunction double-membrane organelles and downregulation transcription factor ATF6. CRISPR/Cas9-mediated inactivation ATF6 in hMSCs, not embryonic cells adipocytes, results premature cellular aging, characteristic loss endomembrane homeostasis. Transcriptomic analyses uncover type-specific...
Cockayne syndrome (CS) is a rare autosomal recessive inherited disorder characterized by variety of clinical features, including increased sensitivity to sunlight, progressive neurological abnormalities, and the appearance premature aging. However, pathogenesis CS remains unclear due limitations current disease models. Here, we generate integration-free induced pluripotent stem cells (iPSCs) from fibroblasts patient bearing mutations in CSB/ERCC6 gene further derive isogenic gene-corrected...
Age-dependent loss of skeletal muscle mass and function is a feature sarcopenia, increases the risk many aging-related metabolic diseases. Here, we report phenotypic single-nucleus transcriptomic analyses non-human primate aging. A higher transcriptional fluctuation was observed in myonuclei relative to other interstitial cell types, indicating susceptibility fiber We found downregulation FOXO3 aged muscle, identified as hub transcription factor maintaining homeostasis. Through establishment...
It has previously been shown that environmental enrichment can enhance structural plasticity in the brain and thereby improve cognitive behavioral function. In this study, we reared developmentally noise-exposed rats an acoustic-enriched environment for ∼4 weeks to investigate whether or not could restore degraded neuronal processing of sound frequency. We found had significantly elevated frequency discrimination thresholds compared with age-matched naive rats. Environmental acoustic nearly...
Sarcopenia, a skeletal muscle disorder in which loss of mass and function progresses with age, is associated increased overall frailty, risk falling mortality the elders. Here, we reveal that SESN1 safeguards from ageing downstream longevity gene FOXO3, recently reported geroprotector primate muscle. Knockdown mimicked human myotube phenotypes observed FOXO3-deficient myotubes, whereas genetic activation alleviated senescence. Of note, was identified as protective secretory factor against...
Hsp90 is a target for anti-cancer drug development. Both the conformational events tuned by ATP/ADP and co-chaperones chaperoning cycle timing are required Hsp90's fully functional display. Interfering with either one of or will down-regulate function. In this manuscript, non-covalent allosteric modulators (SOMCL-16-171 SOMCL-16-175) targeting Hsp90α's middle domain (Hsp90M) were developed first time. Multiple techniques then applied to characterize interactions between two active compounds...
Molecular chaperones are key components of protein quality control system, which plays an essential role in controlling homeostasis. Aha1 has been identified as a co-chaperone Hsp90 known to strongly accelerate Hsp90's ATPase activity. Meanwhile, it is reported that could also act autonomous chaperone and protect stressed or disordered proteins from aggregation. Here, this article, series vitro experiments were conducted verify whether non-Hsp90-dependent holdase activity elucidate the...
Multiple myeloma (MM), the second most common haematological malignancy, is currently incurable because patients often develop multiple drug resistance and experience subsequent relapse of disease. This study aims to identify a potential therapeutic agent that can counter bortezomib (BTZ) in MM. DCZ0358, novel alkaloid compound, found exert potent cytotoxic effects against BTZ-resistant MM cells vivo vitro. The anti-myeloma activity DCZ0358 associated with inhibition cell proliferation,...
Aha1 is the only co-chaperone known to strongly stimulate ATPase activity of Hsp90. Meanwhile, besides well-studied function, human has also been demonstrated exhibit chaperoning against stress-denatured proteins. To provide structural insights for a better understanding Aha1’s and chaperone-like activities, nuclear magnetic resonance (NMR) techniques were used reveal unique structure internal dynamics features full-length Aha1. We then found that, in solution, both two domains presented...