The effects of dietary probiotic supplementation with viable Bacillus subtilis and amyloliquefaciens spores on sow performance, immunity, gut functional status biofilm formation by bacteria in piglets at weaning were investigated. Ninety-six sows reared a continuous farrowing system for one full cycle fed gestation diets during the first 90 d pregnancy lactation until end lactation. basal diet without probiotics (control; n = 48) or supplemented (1.1 × 109 CFU/kg feed) (probiotic; 48). At 7...

Two different diets able to induce dietary hyperammonaemia (a methionine-choline-deficient (MCD) diet and a methionine-deficient enriched with ammonium acetate (MAD + 20% acetate)) were tested in rat model. The shown have modes of action, inducing significant (HA) growth retardation the rats, metabolic consequences. MCD resulted development endogenous HA, decrease bilirubin levels an increase hepatic fat content. In contrast, MAD increased circulating ALP haptoglobin decreased liver mass....

Abstract Revealing the multifocal mechanisms affecting cross-talk between Clostridium perfringens pathogenesis and host response is an urgent need in poultry industry. Herein, activity of Cannabis sativa -derived cannabidiol (CBD) selenium nanoparticles (Nano-Se) modulating to challenge was investigated broiler chickens subjected a mild infection model. The infected exhibited no clinical manifestations, confirming potential hazard pathogen transmission food chain commercial sector. However,...

Additives with bioactive properties can improve chickens' gut health. This study investigated the physiological status of gut, including its morphological structure and microbiome activities in chickens fed diets supplemented phytogenic ingredients hops (Anta®Phyt) or a mixture free butyrate acidifier gluconic acid (PreAcid). In this study, 1155 broilers were distributed to three dietary treatments 5 replicate pens per treatment, 77 birds each. Anta®Phyt was added at 400/300/200/200 mg/kg...

Abstract BACKGROUND Faba bean (FB) can be used in poultry diets as a protein source alternative to soybean meal. This study evaluated the effect of extrusion low‐tannin FB and phytase supplementation on performance, phosphorus (P) nitrogen (N) retention, gut microbiota activity broiler chickens. Raw or extruded meal was included diet at 300 g kg −1 . The experimental treatments additionally differed nutrient level (adequate, calcium, P, reduced) supplementation. RESULTS Broiler performance...

Abstract Selenium is an essential element that important for many metabolic processes. Feed components used in chicken nutrition, especially cereals, may be deficient selenium, hence selenium supplementation necessary. Taking into account the progress breeding, and thus higher demand of birds this element, it seems obvious to investigate increased dose diet chickens. The aim study was evaluate effect feed enriched with different forms at 0.5 mg/kg on profile metabolism fatty acids breast...

Abstract Arginine (Arg), lysine (Lys), and methionine (Met) can be used to support the health status of turkeys. The present study investigated selected performance, gut integrity, immunological parameters in turkeys reared optimal or challenge conditions. experiment lasted for 28 days, it had a completely randomized 2 × 3 factorial design with two levels dietary Arg, Lys Met (high low) Clostridium perfringens ( C. ), Escherichia coli lipopolysaccharide (LPS) no (placebo). Increased...

Abstract No study has demonstrated the use of dietary Cannabis -derived cannabidiol (CBD) to alter stress response in chickens or examined its effects on meat volatile compounds (VOCs). Here, we subjected dysbiosis via C. perfringens infection Escherichia coli lipopolysaccharide (LPS) treatment and investigated potential link between VOCs cecal bacterial activity ameliorative effect CBD. The production short-chain fatty acids (SCFAs) was closely correlated with VOCs. CBD supplementation...

The possible existence of an acini-islet-acinar (AIA) reflex, involving mutual amylase and insulin interactions, was investigated in the current acute experiment on pigs.

Peroxisome proliferator-activated receptors (PPARs) are important targets for cannabidiol (CBD), which mediate many of its biological actions. The hypothesis the present research assumed that PPARs affect gut response to different challenge factors in chickens (C. perfringens vs lipopolysaccharides (LPS) from E. coli), and CBD can pathways this response. study proved significantly expression level PPARα (P=0.001) selected genes determining barrier function. A positive correlation...

Here, we demonstrated the potential of Cannabis-derived cannabidiol (CBD) and nanosized selenium (nano-Se) for modulation microvascularization muscle fiber lesions in superficial breast C. perfringens-challenged chickens. The administration CBD resulted a decreased number atrophic fibers (3.13 vs. 1.13/1.5 mm2) compared with control, whereas nano-Se or both substances split (4.13 1.55/1.5 lower necrotic myofibers (2.38 0.69/1.5 than positive control. There was significantly higher capillary...

The effects of the different dietary levels Arginine (Arg) in low- and high-methionine (Met) diets on meat quality myofibrillar protein profile breast muscles from turkeys were determined. experiment had a completely randomized 3 × 2 factorial design with three Arg (90%, 100%, 110%) relative to Met (30% or 45%). At 42 days age, eight each treatment sacrificed; pH value was measured at 48 h post-slaughter, color according CIE L*a*b* system. SDS-PAGE method performed investigate muscle....

Preterm infants are most at risk of iron deficiency. However, our knowledge the regulation homeostasis in preterm is poor. The main goal research was to develop and validate an animal model human prematurity assess status infants. We performed a cesarean section on sows 109th day pregnancy, which corresponds last trimester pregnancy. piglets showed decreased body weight, red blood cell indices, plasma level transferrin saturation. Interestingly, higher hepatic splenic non-heme content...

Peroxisome proliferator-activated receptors (PPARs) are important targets for cannabidiol (CBD), which mediate many of its biological actions. The hypothesis the present research assumed that PPARs affect gut response to different challenge factors in chickens (

With the current state of knowledge regarding disorders facial bone development, including anodontia, development a suitable animal model for preclinical studies is essential. The agenesis dental buds occurs in about 25% human population. Prospects treatment include use growth factors, stem cells, and bioengineering. This study aimed to investigate influence tooth absence on growth, develop technique application factors developing bone, analyze comparative effect selected active proteins...

The aim of this study was to elucidate the impact porcine pancreatic enzymes (Creon® pancrelipase) in comparison microbial-derived alpha amylase (MD amylase) on small intestine wall structure, mucosal glycogen accumulation, and enterocyte turnover. enzyme supplementation explored 18 pigs with surgically induced exocrine insufficiency (EPI). Four healthy served as control group. EPI led reduced villus length, crypt depth, thickness mucosa muscularis layers compared those pigs. All these...

Butyrate, a by-product of gut bacteria fermentation as well the digestion fat in mother’s milk, exerts wide spectrum beneficial effects gastrointestinal tissues. The present study aimed to determine sodium butyrate on small intestine contractility neonatal piglets. Piglets were fed milk formula alone (group C) or supplemented with B). After 7-day treatment period, isometric recordings whole-thickness segments duodenum and middle jejunum obtained by electric field stimulation under influence...

The transplantation of the pancreas is recommended in diabetes with complications. combination cell biology and 3D-bioprinting can create organs vasculature which should be functional. There are issues to solved: leakage, thrombosis, enhancement against high pressure, connecting organ recipient. purpose this study was demonstrate bionic use dECM-derived biomaterials stable flow through vitro large animal studies. Materials methods: Mathematical analysis designed performed. 3D bioprinted 2...

The first milestone of a new therapy for the treatment T1D is development 3D bioprinted petals consisting of: pancreatic islets and biomaterials. aim study was to demonstrate functionality in vivo studies on large animals. Material Methods: Domestic pigs were research model. animals divided into 4 groups:(1)healthy (Control; n=3);(2) with after pancreatectomy, treated insulin (T1D;n=3);(3) pancreatectomy autotransplantation liver (LIVER; n=3);(4) which autotransplanted bionic (3D-PETALS;...

Introduction: The first milestone of a new therapy for the treatment T1D is development 3D bioprinted petals consisting of: pancreatic islets and biomaterials. aim study was to demonstrate functionality in vivo studies on large animals. Materials Methods: Domestic pigs were research model. animals divided into 4 groups:(1)healthy (Control; n=3);(2) with after pancreatectomy, treated insulin (T1D;n=3);(3) pancreatectomy autotransplantation liver (LIVER; n=3);(4) which autotransplanted bionic...

Purpose: The transplantation of the pancreas is recommended in diabetes with complications. combination cell biology and 3D-bioprinting can create organs vasculature which should be functional. dECM derived biomaterials shoved superior functionality when bioprinted pancreatic islets. In fully vascular organ or macro-device, there are issues to solved: leakage, thrombosis, enhancement against high pressure, connecting recipient. purpose this study was demonstrate bionic as a macro-device for...

Introduction: There is lack organs for transplantation. The transplantation of the pancreas recommended in diabetes with complications. combination cell biology and 3D-bioprinting can create vasculature which should be functional. are some issues to solved: leakage, thrombosis, enhancement against high pressure, connecting organ recipient. purpose this study was 3D-bioprint bionic maintain a stable flow large animals. Material methods: 3 geometries were calculated bio-printed. mechanical...