A5021954051- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Protein Tyrosine Phosphatases
- PI3K/AKT/mTOR signaling in cancer
- Single-cell and spatial transcriptomics
- Blood properties and coagulation
- IL-33, ST2, and ILC Pathways
- Blood disorders and treatments
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Extracellular vesicles in disease
- Galectins and Cancer Biology
- RNA Interference and Gene Delivery
- Eosinophilic Disorders and Syndromes
- DNA Repair Mechanisms
- Mesenchymal stem cell research
- Platelet Disorders and Treatments
- Nanoparticles: synthesis and applications
- MicroRNA in disease regulation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Fungal and yeast genetics research
Istituto Superiore di Sanità
2012-2025
Istituto Nazionale di Fisica Nucleare, Sezione di Roma I
2023
New York Blood Center
2009
Tisch Hospital
2009
Cancer Institute (WIA)
2009
Tisch Cancer Institute
2009
Icahn School of Medicine at Mount Sinai
2009
Ex vivo-generated erythroblasts represent alternative transfusion products. However, inclusion of bovine components in media used for their growth precludes clinical use, highlighting the importance developing culture based on pharmaceutical grade reagents. In addition, because adult blood generates ex vivo lower numbers than cord blood, has been proposed as source choice erythroblast production. To clarify potential to generate vivo, experiments were designed identify factors [stem cell...
Infantile fibrosarcomas (IFS) and congenital mesoblastic nephroma (CMN) are rare myofibroblastic tumors of infancy early childhood commonly harboring the ETV6::NTRK3 gene fusion. IFS/CMN considered as with an 'intermediate prognosis' they locally aggressive, but rarely metastasize, generally have a favorable outcome. A fraction IFS/CMN-related neoplasms negative for rearrangement characterized by other chimeric proteins promoting MAPK signaling upregulation. In large proportion these tumors,...
In recent years, there has been a notable increasing interest surrounding the identification and quantification of nano-sized particles, including extracellular vesicles (EVs) viruses. The challenge posed by dimension these particles makes precise examination significant undertaking. Among different techniques for accurate study EVs, flow cytometry stands out as ideal method. It is characterized high sensitivity, low time consumption, non-destructive sampling, throughput. this article, we...
We developed a new class of inhibitors protein–protein interactions the SHP2 phosphatase, which is pivotal in cell signaling and represents central target therapy cancer rare diseases. Currently available catalytic site or an allosteric pocket but lack specificity are ineffective for disease-associated mutants. Considering that pathogenic lesions cause hyperactivation due to increased levels association with cognate proteins, we peptide-based molecules nanomolar affinity N-terminal Src...
Epithelial-derived alarmins (IL-33, TSLP, and IL-25) play an upstream role in the pathogenesis of asthma. Basophil-derived cytokines are a pivotal component allergic inflammation. We evaluated vitro effects IL-33, IL-25, alone combination with IL-3 on purified peripheral blood human basophils (hBaso) bone marrow-derived mouse (mBaso) modulating production IL-4, IL-13, CXCL8 or equivalents CXCL1 CXCL2. but not TSLP concentration-dependently induced release from hBaso. synergistically...
Type I interferon (IFN-I) evasion by Dengue virus (DENV) is key in DENV pathogenesis. The non-structural protein 5 (NS5) antagonizes IFN-I response through the degradation of signal transducer and activator transcription 2 (STAT2). We developed a K562 cell-based platform, for high throughput screening compounds potentially counteracting NS5-mediated antagonism signaling. Upon with library 1220 approved drugs, 3 previously linked to inhibition (Apigenin, Chrysin, Luteolin) were identified....
Abstract Exosomes are among the most puzzling vehicles of intercellular communication, but several crucial aspects their biogenesis remain elusive, primarily due to difficulty in purifying vesicles with similar sizes and densities. Here we report an effective methodology for labelling small extracellular (sEV) using Bodipy FL C16, a fluorescent palmitic acid analogue. In this study, present compelling evidence that sEV population derived from C16‐labelled cells represents discrete...
Extracellular DNA (exDNA) can be actively released by living cells and different putative functions have been attributed to it. Further, homologous exDNA has reported exert species-specific inhibitory effects on several organisms. Here, we demonstrate experimental evidence, including 1H-NMR metabolomic fingerprint, that the growth rate decline in Saccharomyces cerevisiae fed-batch cultures is determined accumulation of medium. Sequencing such secreted represents a portion entire genome,...
Abstract Immune cell-derived extracellular vesicles (EV) affect tumor progression and hold promise for therapeutic applications. Eosinophils are major effectors in Th2-related pathologies recently implied cancer. Here, we evaluated the anti-tumor activities of eosinophil-derived EV following activation with alarmin IL-33. We demonstrate that IL-33-activated mouse human eosinophils produce higher quantities respect to stimulated IL-5. Following incorporation from (Eo33-EV), but not...
Clear cell RCC (ccRCC) accounts for approximately 75% of the renal cancer cases. Surgery treatment seems to be best efficacious approach majority patients. However, a consistent fraction (30%) cases progress after surgery with curative intent. It is currently largely debated use adjuvant therapy high-risk patients and clinical molecular parameters stratifying beneficiary categories. In addition, advanced forms lacks reliable driver biomarkers appropriated therapeutic choice. Thus, patient...
The cell cycle and the transcriptome dynamics of yeast exposed to extracellular self-DNA during an aerobic batch culture on glucose have been investigated using cytofluorimetric RNA-seq analyses. In parallel, same study was conducted cells growing in presence (heterologous) nonself-DNA. treatment determined a reduction growth rate major elongation diauxic lag phase, as well significant delay achievement stationary phase. This associated with changes dynamics, slower exit from G0 followed by...
In 2005, the European Centre for Validation of Alternative Methods (ECVAM) sponsored a study aimed at evaluating reproducibility (between-laboratory and within-laboratory variability) predictive capacity two in vitro cellular systems — Caco-2/ATCC parental cell line Caco-2/TC7 clone estimating oral fraction absorbed (Fa) humans. Two laboratories, both which had experience with Caco-2 cultures, participated study. Ten test chemicals documented vivo absorption data were selected. Atenolol,...
Studies in mice indicated that activation of the erythroid stress pathway requires presence both soluble KIT ligand (KITL) and glucocorticoid receptor (GR). To clarify relative role KITL GR erythropoiesis humans, biological activities full length- (fl-, 26–190 aa), carboxy-terminus truncated (tr-, 26–162 aa) human (hKITL) murine (mKITL) cultures cord blood (CB) mononuclear cells (MNCs) CD34pos mimic either steady state (growth factors alone) or plus dexamethasone [DXM]) erythropoeisis were...
Monocytes and macrophages utilize the class A B scavenger receptors to recognize perform phagocytosis of invading microbes before a pathogen-specific immune response is generated. HIV-1 Nef protein affects innate system impairing oxidative burst phagocytic capacity macrophages. Our data show that exogenous recombinant myristoylated induces marked CD36 downregulation in monocytes from Peripheral Blood Mononuclear Cells, Monocyte-Derived Macrophages (MDMs) differentiated by cytokines MDMs...
In Human Erythroid Massive Amplification (HEMA) cultures, AB mononuclear cells (MNC) generate 1-log more erythroid (EBs) than the corresponding <svg style="vertical-align:-0.17555pt;width:53.275002px;" id="M1" height="11.8375" version="1.1" viewBox="0 0 53.275002 11.8375" width="53.275002" xmlns="http://www.w3.org/2000/svg"> <g transform="matrix(1.25,0,0,-1.25,0,11.8375)"> transform="translate(72,-62.53)"> <text transform="matrix(1,0,0,-1,-71.95,62.75)"> <tspan style="font-size: 12.50px; "...
Erythropoiesis is a tightly regulated process which becomes decoupled from its normal differentiation program in patients with polycythemia vera (PV). Somatic mutations JAK2 are commonly associated this myeloid proliferative disorder. To gain insight into the molecular events that required for abnormally developing erythroid cells to escape dependence on growth signals, we performed vitro expansion of mature erythroblasts (ERY) seven healthy donors and polycythemic presence IL3, EPO, SCF 10,...
Abstract Glioblastoma, isocitrate dehydrogenase‐wildtype (GB), is the most common and aggressive primary brain malignancy with poor outcome. Immune checkpoint inhibitors (ICIs) have been tested in GB and, despite disappointing results, identification of a small subgroup responders underlies need to improve our understanding tumour microenvironment (TME) immunity. This study aimed determine whether expression selected immune checkpoints on tissue‐resident memory T cells (Trm) may predict...
Ex vivo expanded erythroblasts (EBs) may serve as advanced transfusion products provided that lodgment occurs in the macrophage-niche of marrow permitting maturation. EBs from adult and cord blood expressed receptors (CXCR4, VLA-4, P-selectin ligand 1) necessary for interaction with macrophages. However, 4-days following to intact NOD/SCID/IL2R<mml:math...
Personalised medicine in oncology needs standardised immunological assays. Flow cytometry (FCM) methods represent an essential tool for immunomonitoring, and their harmonisation is crucial to obtain comparable data multicentre clinical trials. The objective of this study was design a workflow able address the most effective issues contributing intra- interoperator variabilities project. Italian National Institute Health (Istituto Superiore di Sanità, ISS) managed multiparametric flow...