Src kinase mediates growth factor signaling and causes oncogenic transformation, which includes dramatic changes in the actin cytoskeleton, cell shape, motility. Cortactin was discovered as a substrate for Src. How phosphorylation of cortactin can enhance assembly is unknown. Here, using an system reconstituted from purified components, we demonstrate first time biochemical mechanism by affects assembly. The adaptor Nck important component system, linking phosphorylated with neuronal WASp...

Osteoclasts are essential for bone dynamics and calcium homeostasis. The cells form a tight seal on the surface, onto which they secrete acid proteases to resorb bone. is associated with ring of actin filaments. Cortactin, c-Src substrate known promote Arp2/3-mediated assembly in vitro, expressed osteoclasts localizes sealing ring. To address role cortactin osteoclasts, we depleted by RNA interference. Cortactin-depleted displayed complete loss resorption no formation zones. On nonosteoid...

Glaucomatous axon injury occurs at the level of optic nerve head (ONH) in response to uncontrolled intraocular pressure (IOP). The temporal ONH astrocytes (glial cells responsible for axonal support) elevated IOP remains unknown. Here, we evaluate actin-based astrocyte extensions and integrin-based signaling within 8 hours elevation a rat model. 60 mm Hg was achieved under isoflurane anesthesia using anterior chamber cannulation connected saline reservoir. astrocytic extension orientation...

Purpose.: To determine if astrocyte processes label for actin and to quantify the orientation of astrocytic within optic nerve head (ONH) in a rat glaucoma model.

Purpose: We determine if several hours of controlled elevation IOP (CEI) will produce the optic nerve head (ONH) gene expression changes and (ON) damage pattern associated with early experimental glaucoma in rats. Methods: The anterior chambers anesthetized rats were cannulated connected to a reservoir elevate IOP. Physiologic parameters monitored. Following CEI at various recovery times, ON cross-sections graded for axonal injury. Anterior ONHs collected 0 10 days following RNA extracted...

Optic nerve head (ONH) astrocytes provide support for axons, but exhibit structural and functional changes (termed reactivity) in a number of glaucoma models. The purpose this study was to determine if ONH astrocyte reactivity is axon-dependent.Using rats, we combine retrobulbar optic transection (ONT) with acute controlled elevation intraocular pressure (CEI), induce total axon loss reactivity, respectively. Animals were euthanized immediately or 1 day post CEI, the presence absence ONT....

Purpose: Glaucoma is common in patients with the Boston type 1 keratoprosthesis (KPro). Because of inability to perform corneal applanation KPro patients, digital palpation currently mainstay for assessment intraocular pressure (IOP). The purpose this study was determine whether Diaton tonometer, which estimates IOP through transpalpebral scleral indentation, can reasonably detect high when compared Goldmann tonometry (GAT) ocular hypertension, glaucoma, or glaucoma tube shunts. If reliable...

Purpose: To clarify the optic nerve head (ONH) gene expression responses associated with a single, axon-damaging exposure to elevated IOP in relation composite cellular events previously identified models of chronically IOP. Methods: Anesthetized rats were exposed unilaterally an 8-hour pulse-train controlled elevation (PT-CEI) at 60 mm Hg, while others received normotensive CEI 20 Hg. ONH RNA was harvested 0 hours and 1, 2, 3, 7, 10 days after either from naïve animals. sequencing performed...

To evaluate the outcomes of surgical removal premacular fibrosis (PMF) with peeling internal limiting membrane (ILM). Determination factors associated favorable (visual acuity between 20/20 and 20/40).Retrospective chart review, evaluating visual in 38 patients who underwent pars plana vitrectomy (PPV) triamcinolone-assisted ILM by a single surgeon December 2003 2004. Data collected included before 3 months after surgery, at final follow-up, as well complications (cataract formation,...

The effects of saffron ethanolic extract and its constituent, safranal, on the acquisition expression morphine-induced place preference (CPP) in male Swiss Webster mice (20-25 g) were investigated present study. An unbiased conditioning method was applied for assessment morphine reward properties. safranal administered intraperitoneally (i.p.) during (acquisition) or after induction (expression) CPP. In a pilot study, alone to animals assess if they have any Subcutaneous (s.c.) (4 8 mg...

Patch graft erosion and implant exposure is a known complication of glaucoma drainage device (GDD) surgery. Recently, electron beam (e-beam) irradiated corneal tissue ha s become available; however, limited data exist on the rates for e-beam grafts compared to traditional scleral after GDD This retrospective study examines records 253 eyes from 225 adult subjects who underwent surgery with either or at Casey Eye Institute by five surgeons between April 22, 2014 October 11, 2017. Surgical...

Cryogens have been used to freeze living tissue for the purpose of treating benign and malignant lesions. Within last century, ophthalmologists found cryotherapy be useful in a variety ocular pathologies. Here, we review history cryotherapy, its introduction field ophthalmology, proposed mechanism action, current applications surface intraocular eye disease.

The self-association of the tranquilizer chlorpromazine hydrochloride in aqueous solution was studied. Using isothermal titration calorimetry, critical micelle concentration and solvent−micelle interactions were evaluated as a function temperature, ionic strength, pH. enthalpy demicellization, ΔHdemic, directly measured from experimental values, which showed that spontaneous demicellization is highly endothermic process under all conditions thermodynamic parameters subsequently calculated...

Glaucoma is a known complication of uveitis, and may require glaucoma tube shunt implantation for intraocular pressure (IOP) control. The success in the setting local ocular steroid depot uveitic remains unknown. purpose this study was to determine whether patients who underwent combined (Ahmed) fluocinolone acetonide (Retisert™, Bausch + Lomb, Bridgewater, NJ, USA) have superior outcomes compared with Ahmed implants only glaucoma. All participants were studied retrospectively alone or...

To describe the clinical findings of two patients with reversible ocular hypertension secondary to endogenous hypercortisolism.Retrospective, observational case series.A 65-year-old man (patient 1) and a 21-year-old woman 2) were both found have Cushing's syndrome after presentation our clinic elevated intraocular pressures (IOP).Clinical histories, ophthalmic examinations including IOP measurements, optical coherence tomography retinal nerve fiber layer, visual field testing, magnetic...

Small molecule delivery to the optic nerve would allow for exploration of molecular and cellular pathways involved in normal physiology neuropathies such as glaucoma, provide a tool screening therapeutics animal models. We report novel surgical method small drug head (ONH) rodent model. In proof-of-principle experiments, we delivered cytochalasin D (Cyt D; filamentous actin inhibitor) junction superior globe rats target actin-rich astrocytic cytoskeleton ONH. Cyt was quantified by liquid...