A5043782637- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Cancer Mechanisms and Therapy
- Pancreatic and Hepatic Oncology Research
- Immune cells in cancer
- Cardiac Ischemia and Reperfusion
- Cancer Research and Treatments
- Air Quality and Health Impacts
- Cancer Cells and Metastasis
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Climate Change and Health Impacts
- Adenosine and Purinergic Signaling
- Sphingolipid Metabolism and Signaling
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Chromatin Remodeling and Cancer
- Phytochemicals and Antioxidant Activities
- Virus-based gene therapy research
- Autophagy in Disease and Therapy
- Connexins and lens biology
- Genomics, phytochemicals, and oxidative stress
- Pharmacological Receptor Mechanisms and Effects
APLA Health
2020-2025
Peking University
2021-2024
Clemson University
2024
UCLA Health
2024
Harbin Medical University
2023-2024
Zhongshan Hospital of Xiamen University
2023-2024
University of California, Los Angeles
2012-2023
Tianjin University of Science and Technology
2022-2023
Henan University of Science and Technology
2023
Zhengzhou University
2021-2022
Although enhanced cardiac matrix metalloproteinase (MMP)-2 synthesis has been associated with ventricular remodeling and failure, whether MMP-2 expression is a direct mediator of this process unknown. We generated transgenic mice expressing active driven by the alpha-myosin heavy chain promoter. At 4 mo hearts demonstrated transgene, myocyte hypertrophy, breakdown Z-band registration, lysis myofilaments, disruption sarcomere mitochondrial architecture, fibroblast proliferation. Hearts from...
Introduction Pancreatic ductal adenocarcinoma (PDAC) is projected to rise the second leading cause of U.S. cancer-related deaths by 2020. Novel therapeutic targets are desperately needed. MicroRNAs (miRs) small noncoding RNAs that function suppressing gene expression and dysregulated in cancer. miR-21 overexpressed PDAC tumor cells (TC) associated with decreased survival, chemoresistance invasion. Dysregulation miR regulatory networks tumor-associated fibroblasts (TAFs) have not been...
Objective: There is a widely held view that, due to high fat content, snacking on nuts will lead weight gain, ultimately causing unhealthy changes in lipid profiles. This study designed the effects of pistachio snack consumption body and levels obese participants under real-world conditions.Methods: Participants were randomly assigned consume 1 2 isocaloric reduction diets for 12 weeks, with each providing 500 cal per day less than resting metabolic rate. Each diet included an afternoon...
Pancreatic ductal adenocarcinoma (PDAC) has a characteristically dense stroma comprised predominantly of cancer-associated fibroblasts (CAF). CAFs promote tumor growth, metastasis, and treatment resistance. This study aimed to investigate the molecular changes functional consequences associated with chemotherapy PDAC CAFs. Chemoresistant immortalized (R-CAF) were generated by continuous incubation in gemcitabine. Gene expression differences between treatment-naïve (N-CAF) R-CAFs compared...
In traumatic brain injury (TBI), a diversity of resident and peripherally derived myeloid cells have the potential to worsen damage and/or assist in healing. We define heterogeneity microglia macrophage phenotypes during TBI wild-type (WT) mice Ccr2-/- mice, which lack influx following are resistant damage. use unbiased single-cell RNA sequencing methods uncover 25 microglia, monocyte/macrophage, dendritic cell subsets acute normal brains. find alterations transcriptional profiles compared...
Emerging science has shown the effect of oxidation products and inflammation on atherogenesis carcinogenesis. Cooking hamburger meat can promote formation malondialdehyde that be absorbed after ingestion.We studied an antioxidant spice mixture while cooking its effects plasma urinary concentrations.Eleven healthy volunteers consumed 2 kinds burgers in a randomized order: one burger was seasoned with blend, not blend. The production concentrations urine ingestion were measured by...
Strawberries are known to contain antioxidants, but the significance of ingesting antioxidant-rich fruits remains be established. In order determine whether consumption strawberries impacted measures in vivo antioxidant capacity, frozen (250 g) were administered daily for 3 weeks 21 healthy female volunteers. Compliance was confirmed by quantitating pelargonidin-glucuronide, urolithin A-glucuronide, and 2,5-dimethyl-4-hydroxy-3-[(2)H]furanone-glucuronide plasma urine liquid...
Exogenous sphingosine 1-phosphate (S1P) is an effective cardioprotectant against ischemic injury. We have investigated the hypothesis that S1P also important endogenous released during both preconditioning (IPC) and postconditioning (IPOST). IPC of ex vivo rat hearts was instituted by two cycles 3 min ischemia-5 reperfusion prior to 40 index ischemia then reperfusion. resulted in 70% recovery left ventricular developed pressure (LVDP) upon a small infarct size (10%). VPC23019 (VPC), specific...
Pancreatic cysts are a group of lesions with heterogeneous malignant potential. Currently, there no reliable biomarkers to aid in cyst diagnosis and classification. The objective this study was identify potential microRNA (miR) endoscopically acquired pancreatic fluid that could be used distinguish between benign, premalignant, cysts.A list candidate miRs developed using whole-genome expression array analysis cancer (pancreatic ductal adenocarcinoma) nonmalignant samples overlapped existing...
Significance Pancreatic cancer is notoriously treatment resistant. These tumors rely on lysosome-dependent recycling pathways to generate substrates for metabolism, which are inhibited by chloroquine (CQ) and its derivatives. However, clinical efficacy of CQ as a monotherapy or combined with standard-of-care regimens has been limited. Using an unbiased kinome screen, we identify replication stress induced vulnerability due impaired de novo nucleotide biosynthesis find that combination...
Purine nucleoside phosphorylase (PNP) enables the breakdown and recycling of guanine nucleosides. PNP insufficiency in humans is paradoxically associated with both immunodeficiency autoimmunity, but mechanistic basis for these outcomes incompletely understood. Here, we identify two immune lineage-dependent consequences inactivation dictated by distinct gene interactions. During T cell development, synthetically lethal downregulation dNTP triphosphohydrolase SAMHD1. This interaction requires...
Aims: Extracellular vesicles from Lacticaseibacillus paracasei PC-H1 have antiproliferative activity of colon cells, but the effect on glycolytic metabolism cancer cell remains enigmatic. The authors investigated how extracellular (LpEVs) inhibit growth cells by affecting tumor metabolism. Materials & methods: HCT116 were treated with LpEVs and then differentially expressed genes analyzed transcriptome sequencing, sequencing results confirmed in vivo vitro. Results: entered inhibited their...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is characterized by an immunosuppressive tumor immune microenvironment (TIME) where combinations of chemotherapy and checkpoint blockade (ICB) have shown limited efficacy. Standard care FOLFIRINOX (FFX) promotes replication stress in cells triggering synthesis 2’-3’ cGAMP, intercellular mediator STING activation nearby leukocytes. induces Type 1 Interferon (IFN) production a cascade anti-tumor responses. In parallel, dying release ATP that...
Abstract Fractionation of cytosolic sphingosine kinase (SKase) activity by gel filtration chromatography gave rise to a 96‐kDa peak that contained only the SK2 form SKase (by Western analysis) and broad ca. 46 kDa SK1 forms. appeared have bound accessory protein. When tested with classic inhibitor dimethylsphingosine (DMS), was extensively inhibited; however, not inhibited but unexpectedly activated. Activation result DMS enhancing affinity enzyme for sphingosine, and, at low concentrations...
Treatment of patients with locally advanced/borderline resectable (LA/BR) pancreatic ductal adenocarcinoma (PDAC) is not standardized.OBJECTIVE To (1) perform a detailed survival analysis our institution's experience LA/BR PDAC who were downstaged and underwent surgical resection (2) identify prognostic biomarkers that may help to guide decision for the use adjuvant therapy in this patient subgroup. DESIGN, SETTING, AND PARTICIPANTSRetrospective observational study 49 consecutive from single...
MicroRNA-21 (miR-21) is upregulated and inversely associated with survival in many cancer types, including pancreatic ductal adenocarcinoma (PDAC). We studied the predictive value of miR-21 levels for gemcitabine or 5-fluorouracil (5-FU) response tumor cells (TCs) fibroblasts (CAFs) a cohort PDAC patients from RTOG 9704 trial.MiR-21 expression CAFs TCs, determined by situ hybridization, 229 subset was correlated (i) histopathology characteristics using chi-square test; (ii) patient overall...
Hydrothermal carbonization (HTC) has a unique advantage over conventional processes to convert wet biomass into solid fuel without the need dewater and dry biomass. A byproduct of HTC process is aqueous phase (AP), rich in organic inorganic compounds. The AP, if discharged proper treatment, can cause environmental pollution. In this study, mono-, co-, stepwise-culture fungi (Penicillium sp.) microalgae (Chlorella on AP from same fungi-microalgae strains was investigated. results showed that...
We determine that type I interferon (IFN) response biomarkers are enriched in a subset of pancreatic ductal adenocarcinoma (PDAC) tumors; however, actionable vulnerabilities associated with IFN signaling have not been systematically defined. Integration phosphoproteomic analysis and chemical genomics synergy screen reveals activates the replication stress kinase ataxia telangiectasia Rad3-related protein (ATR) PDAC cells sensitizes them to ATR inhibitors. triggers cell-cycle arrest S-phase,...
These experiments identify an inward rectifier K+ (Kir) channel expressed in mouse cortical and white matter astrocytes at the molecular level. Messenger RNA for one of known Kir genes, Kir4.1, is present much higher levels primary culture than other family members. In culture, level Kir4.1 mRNA lower proliferating cells cultured 16 h under hypoxic conditions, compared to confluent cells. Partial differentiation with dibutyryl cAMP or by coculture neurons has no effect on situ hybridization...
The cardioprotective effectiveness of low-dose pyrroloquinoline quinone (PQQ, 3 mg/kg) was compared with metoprolol, a β 1 -selective adrenoceptor antagonist. Rats underwent 30 minutes left anterior descending coronary artery occlusion and 2 hours reperfusion. Metoprolol and/or PQQ were given at the onset reperfusion to mimic clinical treatment. reduced infarct size protected against ischemia-induced ventricular dysfunction after reper-fusion. Combined therapy augmented developed pressure...
Ischemic pre- and postconditioning protect ex vivo rat hearts from ischemia/reperfusion injury by promoting the release of cardioprotective agents an unknown mechanism. Because P2X( 7) purinergic receptors are known to combine with pannexin-1 form channels that allow adenosine triphosphate (ATP) cells, we hypothesized these have a role in multiple cardioprotectants during ischemic preconditioning (IPC). Addition either hemichannel blocker (5 micromol/L carbenoxolone [CBX] or 0.4 mefloquine...