A5020146950- Prostate Cancer Treatment and Research
- Cancer, Stress, Anesthesia, and Immune Response
- Skin Protection and Aging
- Ion Transport and Channel Regulation
- Estrogen and related hormone effects
- Renal cell carcinoma treatment
- Cancer, Hypoxia, and Metabolism
- Immune cells in cancer
- Radiopharmaceutical Chemistry and Applications
- Ethics in Clinical Research
- Eicosanoids and Hypertension Pharmacology
- Cancer Genomics and Diagnostics
- Inflammatory mediators and NSAID effects
- Advanced Breast Cancer Therapies
- Biomedical Ethics and Regulation
- Lung Cancer Treatments and Mutations
- Epigenetics and DNA Methylation
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Kruppel-like factors research
- Renal and related cancers
- Chemokine receptors and signaling
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- Phytochemical compounds biological activities
University of California, Los Angeles
2016-2025
APLA Health
2018-2025
University of California, San Francisco
2010-2023
UCSF Helen Diller Family Comprehensive Cancer Center
2023
VA Greater Los Angeles Healthcare System
2013
Technical Institute of Physics and Chemistry
2013
UCLA Health
2012
University of Pittsburgh
2003-2011
Broad Center
2010
University of Virginia
2009
Growing evidence suggests that tumor-associated macrophages (TAM) promote cancer progression and therapeutic resistance by enhancing angiogenesis, matrix-remodeling, immunosuppression. In this study, prostate under androgen blockade therapy (ABT) was investigated, demonstrating TAMs contribute to disease recurrence through paracrine signaling processes. ABT induced the tumor cells express macrophage colony-stimulating factor 1 (M-CSF1 or CSF1) other cytokines recruit modulate macrophages,...
Cancer cells exhibit increased use of nutrients, including glucose and glutamine, to support the bioenergetic biosynthetic demands proliferation. We tested small-molecule inhibitor glutaminase CB-839 in combination with erlotinib on epidermal growth factor receptor (EGFR) mutant non-small cell lung cancer (NSCLC) as a therapeutic strategy simultaneously impair glutamine utilization thereby suppress tumor growth. Here, we show that cooperates drive energetic stress activate AMP-activated...
Lung premalignancy and early-stage adenocarcinoma use a mechanism of glucose transport that can be targeted for early diagnosis treatment.
Abstract KLF4/GLKF4 is a transcription factor that can have divergent functions in different malignancies. The role of KLF4 prostate cancer etiology remains unclear. We recently reported small double-stranded RNA induce gene expression by targeting promoter sequence phenomenon referred to as activation (RNAa). In this study, we examine levels tissue and utilize RNAa tool for overexpression investigate its function. Expression analysis indicated significantly downregulated cell lines compared...
Patients with chronic kidney disease (CKD) have elevated circulating levels of trimethylamine N-oxide (TMAO), a metabolite derived from gut microbes and associated cardiovascular diseases. High TMAO its dietary precursor, choline, predict increased risk for development CKD in apparently healthy subjects, studies mice fed or choline suggest that can contribute to impairment renal fibrosis. Here we examined the interactions between TMAO, disease, mouse models. We observed while female...
Abstract Early cancer detection by cell-free DNA faces multiple challenges: low fraction of tumor DNA, molecular heterogeneity cancer, and sample sizes that are not sufficient to reflect diverse patient populations. Here, we develop a approach address these challenges. It consists an assay, cfMethyl-Seq, for cost-effective sequencing the methylome (with > 12-fold enrichment over whole genome bisulfite in CpG islands), computational method extract methylation information diagnose patients....
Plasma cell-free DNA (cfDNA) is a noninvasive biomarker for cell death of all organs. Deciphering the tissue origin cfDNA can reveal abnormal because diseases, which has great clinical potential in disease detection and monitoring. Despite promise, sensitive accurate quantification tissue-derived remains challenging to existing methods due limited characterization methylation reliance on unsupervised methods. To fully exploit cfDNA, here we present one largest comprehensive high-resolution...
Abstract Coronavirus disease 2019 (COVID-19) poses significant risks for solid organ transplant recipients, who have atypical but poorly characterized immune responses to infection. We aim understand the host immunologic and microbial features of COVID-19 in recipients by leveraging a prospective multicenter cohort 86 age- sex-matched with 172 non-transplant controls. find that higher nasal SARS-CoV-2 viral abundance impaired clearance, lower anti-spike IgG levels. In addition, exhibit...
Parathyroid hormone (PTH) regulates extracellular calcium homeostasis through the type 1 PTH receptor (PTH1R) expressed in kidney and bone. The PTH1R undergoes β-arrestin/dynamin-mediated endocytosis response to biologically active forms of PTH, PTH-(1–34), PTH-(1–84). We now show that amino-truncated do not activate nonetheless induce internalization a cell-specific pattern. Activation-independent proceeds distinct arrestin-independent mechanism is operative cells lacking adaptor protein...
As a transcriptional repressor of E-cadherin, Snail has predominantly been associated with epithelial-mesenchymal transition, invasion, and metastasis. However, other important Snail-dependent malignant phenotypes have not fully explored. Here, we investigate the contributions to progression non-small cell lung cancer (NSCLC).Immunohistochemistry was done quantify localize in human tissues, tissue microarray analysis used correlate these findings survival. NSCLC lines gene-modified stably...
Small cell neuroendocrine carcinoma (SCNC) of the prostate is a variant form cancer that occurs de novo or as recurrent tumor in patients who received hormonal therapy for prostatic adenocarcinoma. It composed pure (NE) cells, but unlike scattered NE cells benign and adenocarcinoma are quiescent, SCNC highly proliferative aggressive, causing death months. In this study, we provide evidence interleukin 8 (IL8)–CXCR2–P53 (TP53) signaling pathway keeps quiescent state normally. While P53...
We report the genetic analysis of a “humanized” hyperlipidemic mouse model for progressive nonalcoholic steatohepatitis (NASH) and fibrosis. Mice carrying transgenes human apolipoprotein E*3‐Leiden cholesteryl ester transfer protein fed “Western” diet were studied on backgrounds over 100 inbred strains. The mice developed hepatic inflammation fibrosis that was highly dependent background, with vast differences in degree Histological showed features characteristic NASH, including...
Background Microstructural MRI has the potential to improve diagnosis and characterization of prostate cancer (PCa), but validation with histopathology is lacking. Purpose To validate ex vivo diffusion-relaxation correlation spectrum imaging (DR-CSI) in microstructural tissue compartments specimens from men PCa by using registered whole-mount digital (WMHP) as reference standard. Materials Methods Men who underwent 3-T robotic-assisted radical prostatectomy between June 2018 January 2019...
The low bioavailability of most phytochemicals limits their anticancer effects in humans. present study was designed to test whether combining arctigenin (Arc), a lignan mainly from the seed Arctium lappa, with green tea (GT) and quercetin (Q) enhances chemopreventive effect on prostate cancer. We performed vitro proliferation studies different cell lines. observed strong synergistic anti-proliferative GT+Q+Arc exposing androgen-sensitive human cancer LNCaP cells. pre-malignant WPE1-NA22...
Acute arterial hypertension provokes a rapid decrease in proximal tubule (PT) Na+ reabsorption, increasing flow to the macula densa, signal for tubuloglomerular feedback. We tested hypothesis, rats, that transport is decreased due redistribution of apical Na+/H+ exchangers and basolateral pumps internal membranes. Arterial pressure was increased 50 mmHg by constricting various arteries. also whether transporter internalization occurred when PT reabsorption inhibited with carbonic anhydrase...
The aim of this study was to test the hypothesis that in vivo administration parathyroid hormone (PTH) provokes diuresis/natriuresis through redistribution proximal tubule apical sodium cotransporters (NHE3 and NaPi2) internal stores inhibition basolateral Na-K-ATPase activity determine whether same cellular signals drive changes transporters. PTH-(1–34) (20 U), which couples adenylate cyclase (AC), phospholipase C (PLC), A 2 (PLA ), or [Nle 8,18 ,Tyr 34 ]PTH-(3–34) (10 PLC PLA but not AC,...
G protein-coupled receptors (GPCRs) mediate the action of many hormones, cytokines, and sensory chemical signals. It is generally thought that receptor desensitization internalization require occupancy activation GPCR. PTH PTHrP (PTH1R) belongs to GPCR class B major regulator extracellular calcium homeostasis. Using kidney distal convoluted tubule cells transfected with a human PTH1R/enhanced green fluorescent protein fusion protein, quantitative, real-time fluorescence microscopy was used...
Stimulation of bone formation by osteoinductive materials is great clinical importance in spinal fusion surgery, repair fractures, and the treatment osteoporosis. We previously reported that specific naturally occurring oxysterols including 20(S)-hydroxycholesterol (20S) induce osteogenic differentiation pluripotent mesenchymal cells, while inhibiting their adipogenic differentiation. Here we report characterization two structural analogues 20S, Oxy34 Oxy49, which inhibit marrow stromal...
Acute hypertension provokes a rapid decrease in proximal tubule sodium reabsorption with basolateral membrane sodium-potassium-ATPase activity and an increase the density of membranes containing apical sodium/hydrogen exchangers (NHE3) [Y. Zhang, A. K. Mircheff, C. B. Hensley, E. Magyar, D. G. Warnock, R. Chambrey, K.-P. Yip, J. Marsh, N.-H. Holstein-Rathlou, McDonough. Am. Physiol.270 ( Renal Fluid Electrolyte Physiol.39): F1004–F1014, 1996]. To determine reversibility specificity these...
Inhibiting cardiac Na pump activity decreases the driving force for Na/Ca exchanger transport that increases cellular Ca stores and contractility. Decreased abundance of pumps would be expected to have same effect as decreased unless there was reciprocal regulation expression oppose effects depressed on intracellular stores. The aim this study test hypothesis is regulated in a fashion Na-K-ATPase number models known altered abundance. In renovascular hypertension, ventricular alpha 1-levels...
The activity of Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase), the sodium pump, which drives active Na+ reabsorption along nephron, varies over an order magnitude, depending on nephron segment, and is increased in outer medullary collecting tubule (MCT) during hypokalemia. aims present study were to assess abundance pump alpha 1- beta 1-subunits dissected segments rat by immunoblotting, determine if 2- or 3-protein could be detected tubules, as suggested Barlet-Bas et al. (C....