Abstract Background Currently, early diagnosis of diabetic nephropathy (DN) remains a major challenge. Thus, more investigations into new DN‐related biomarkers are needed. Methods We employed urinary proteomic approach fluorescence‐based difference gel electrophoresis (DIGE) and mass spectrometry to identify novel in urine samples, which were from type 2 diabetes patients with normoalbuminuria (DM group), microalbuminuria (DN1 macroalbuminuria (DN2 group) control group ( n = 8 each group)....

Summary We performed a multicentre, non‐randomised trial (NCT03533790) to investigate the efficacy of ruxolitinib combined with doxorubicin‐etoposide‐methylprednisolone (Ru‐DEP) regimen as salvage therapy for refractory/relapsed haemophagocytic lymphohistiocytosis (HLH). All patients failing achieve complete or partial response 2 weeks after initial HLH‐94/HLH‐04 relapsed remission were enrolled in study between June 2018 and 2019. The was evaluated initiating Ru‐DEP therapy. Fifty‐four...

Coreopsis tinctoria Nutt is an ethnomedicine widely used in Xinjiang, China. It consumed as a herbal tea by local Uyghur people to treat high blood pressure and diarrhea. Our previous study confirmed that the ethyl acetate extract of (AC) had protective effect on diabetic nephropathy (DN) vivo experiment. Here we aim elucidate mechanism AC marein, main ingredient renal fibrosis inflammation vitro under glucose (HG) conditions. A HG-induced barrier dysfunction model rat mesangial cells...

Identification of the risk predictor diabetic nephropathy (DN) remains a major challenge currently. Thus, proteomic approaches to identify DN-related biomarker were performed.A comparative approach 2-D gel electrophoresis (2-DE) and mass spectrometry biomarkers in urine samples from 12 DN patients (six type 1 six 2 patients) healthy controls. Then, urinary level identified protein was detected by immunoturbidimetry assay 90 with normo-, micro- macroalbuminuria (n = 30 each group), controls.A...

Diabetic nephropathy is a serious complication of diabetes whose development process associated with inflammation, renal hypertrophy, and fibrosis. Coreopsis tinctoria Nutt, traditionally used as healthcare tea, has anti-inflammatory, anti-hyperlipidemia, glycemic regulation activities. The aim our study was to investigate the protective effect ethyl acetate extract C. Nutt (AC) on high-glucose–fat diet streptozotocin (STZ)-induced diabetic rats. A rat model induced by intraperitoneal...

This study investigated the role of JAK/STAT, an important pathway for cytokine signal transduction, in progression chronic glomerular diseases.BALB/c mice received a single intravenous injection adriamycin (10 mg/kg) were sacrificed 2, 4 and 6 weeks later. In second study, treatment with selective JAK2 inhibitor AG490 (15 mg/kg, q.d., i.p.) or vehicle was started 5 days after injection. Functional pathologic markers, inflammatory infiltration, expression pro-inflammatory cytokines...

Abstract We aimed to investigate the relationship between neutrophil lymphocyte ratio (NLR) and nutritional parameters in chronic kidney disease (CKD) patients. In this cross-sectional study, 187 non-dialysis CKD patients were enrolled. Daily dietary energy intake (DEI) daily protein (DPI) assessed by 3-d records. Protein-energy wasting (PEW) was defined as Subjective Global Assessment (SGA) class B C. Spearman correlation analysis, logistic regression analysis receiver operating...

Diabetic nephropathy (DN) is mainly regarded as diabetic glomerulopathy, and its progression tightly correlated with tubular epithelial lesions. However, the underlying molecular mechanisms linking damage glomerulopathy are poorly understood. Methods: We previously reported that upregulation of Bim mediated proximal cell (PTEC) apoptosis was crucial in early stages DN. Herein we modulated expression PTECs subsequently determined podocyte (PC) cytoskeletal arrangement by building a Transwell...

Pathologic diagnosis of stage I idiopathic membranous nephropathy (MN-I) requires electron microscopy or immunohistochemistry that shows a glomerular capillary staining pattern IgG and C3. However, it is not uncommon renal biopsy did obtain sufficient material for C3 in glomeruli largely lost at due to corticosteroid treatment. Since C3d one the final degradation products more stable vivo, we determine if could be used as novel immunohistochemical marker MN-I.74 MN-I patients with proven...

Abstract The present study aims to explore the therapeutic effect of Stefin B on gouty arthritis (GA) and polarization macrophages in mice. B-overexpressed or knockdown M0 were constructed. GA model was established mice by injecting 25 mg/mL MSU, followed a single B-overexpressing adenovirus vector (GA + OE) an empty OE NC). found lowly expressed M1 macrophages. CD206 markedly upregulated IL-10 release signally increased In mice, marked redness swelling observed ankle joint. Dramatical...

Background: IgA nephropathy (IgAN) is a cause of chronic kidney disease (CKD).Tubular atrophy/interstitial fibrosis associated with IgAN prognosis.However, simple tools for predicting pathological lesions remain limited.Our objective was to develop tool evaluating tubular in patients IgAN.Methods: In this cross-sectional study, 410 biopsy-verified were included.The factors the incident interstitial or atrophy confirmed by using logistic regression analysis.A nomogram developed coefficients...

Background The mechanism of immunoglobulin A nephropathy (IgAN) is still unknown. bioinformatics analysis a powerful method to identify the biomarkers and possible therapeutic targets certain disease from related datasets. Methods GSE93973 dataset, obtained Gene Expression Omnibus (GEO) database, was used construct weighted gene co-expression network (WGCNA) filter differentially expressed genes (DEGs). biological process (BP) enrichment among all in key modules analyzed through Ontology...

A bioinspired double network hydrogel scaffold (GCS) loaded with curcumin and N -acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) was prepared. This cyto-favorable could prevent renal fibrosis, promote injury repair regeneration.

Background IgA nephropathy (IgAN) is an immune disease related to oxidative stress and inflammation. It the most common type of glomerulonephritis in world cause chronic kidney end-stage renal (ESRD). The Geriatric Nutritional Risk Index (GNRI) a practical uncomplicated method assess risk morbidity mortality, but its ability IgAN still unclear. Here, we evaluated association between GNRI clinical histologic findings IgAN. Methods In cross–sectional study, included 348 biopsy-verified...

The rate of kidney function decline in patients with diabetic disease (DKD) is known to differ. This study analyzed the clinicopathologic features and related risk factors affecting long-term renal survival Chinese type 2 rapid estimated glomerular filtration (eGFR) decline.In this retrospective descriptive study, 191 DKD were first classified as eGFR decliners slow on basis median slope value (-8.0 mL/min/1.73 m2/year). In total, 96 included analyses subsequently allocated end-stage (ESRD)...

Most patients with systemic lupus erythematosus (SLE) develop nephritis (LN) severe kidney manifestations. Renal fibrosis can be primarily attributed to overproliferation of mesangial cells (MCs), which are subject drug treatment. Nevertheless, the detailed mechanisms remain elusive. We sought identify effect cyclophosphamide (CTX), a commonly used for LN treatment, on MC proliferation and explore its underlying mechanisms. Material/Methods. Cell in mouse tissues were determined by...

To investigate the effects of trimetazidine on renal function in patients with shock.A prospective randomized controlled double-blind study was conducted. 128 shock admitted to intensive care unit (ICU) Guangzhou Red Cross Hospital from April 2011 2013 were enrolled and randomly divided into control group treatment group, each n=64. All received anti-shock treatment, while (20 mg orally, tid) for 7 days, placebo (10 mL sterile water injection, days. The urinary output, serum creatinine...

Abstract Background Low levels of high-density lipoprotein cholesterol (HDL-C) and diabetes are common in patients undergoing peritoneal dialysis (PD). The aim this study was to investigate the association between coexistence with a low level HDL-C first episode dialysis-related peritonitis (PDRP) PD. Methods We retrospectively investigated PD from January 1, 2003, May 31, 2020, four centers. Patients were divided into groups: no comorbidities, only, plus HDL-C. clinical laboratory baseline...