To overcome the drawbacks of in vitro liver testing during drug development, numerous liver-on-a-chip models have been developed. However, current technologies are labor-intensive, lack extracellular matrix (ECM) essential for cells, and a biliary system excreting bile acids, which contribute to intestinal digestion but known be toxic hepatocytes. Therefore, fabrication methods development that above limitations required. Cell-printing technology enables construction complex 3D structures...

The boom of plant phenotype highlights the need to measure physiological characteristics an individual plant. However, continuous real-time monitoring a plant's internal status remains challenging using traditional silicon-based sensor technology, due fundamental mismatch between rigid sensors and soft curved surfaces. Here, first flexible electronic sensing device is reported that can harmlessly cohabitate with continuously monitor its stem sap flow, critical characteristic for analyzing...

Abstract During tumor progression, the size and location of are important factors closely associated with metastatic potential cancer as they largely govern hypoxia angiogenesis. However, despite achievements previous studies, these critical poorly studied, mainly due to lack a flexible technique that can readily control 3D mimicking constructs their spatial relations vasculature. Here, novel tissue‐level platform consisting unit (MCU) perfusable vascular endothelium system (VES) is...

An organ-on-a-chip is a microfluidic device that simulates the microenvironment of organs, facilitating study human physiology and disease mechanisms. Through integration tissue engineering micromachining technologies, it effectively manages cellular implements tissue-specific functions physiological responses with high fidelity. Several factors must be appropriately considered in fabrication an organ-on-a-chip, including choice biomaterials to simulate extracellular matrix (ECM), selection...

Precise in vitro models tissue engineering have attracted the attention of researchers seeking to understand physiological consequences from native tissues as well mechanism diseases vitro. To construct delicate tissue-like models, a proper combination biomimetic materials and biofabrication strategy is required. Conventional biomaterials, such collagens, laminins, synthetic polymers, been widely adapted recapitulation; however, they lack specificity context biophysical properties...

The integration of ZnO nanowire-based energy harvesting devices into flexible polyesters or clothes would have a significant effect on the building block for mechanical from human motions. Moreover, demonstration high output power via doping process opens an important method enhancing power. Here, we report solution-based synthesis Ag-doped nanowires polyester substrates without using any temperature annealing processes. Along with structural and optical characteristics nanowires,...

Liver fibrosis is a critical liver disease which can lead to cirrhosis, cancer, and failure. Among various etiological factors, activated stellate cells are major factor that induce fibrosis. Several studies have presented in vitro models identify drugs for fibrosis; however, there still limitations terms of the 2D culture conditions, random co-culture cells, lack extracellular matrix components. Therefore, 3D fibrosis-on-a-chip was developed with three cell types (hepatocytes, endothelial...

Abstract Objective —To test the hypotheses that kinematic data of sagittal motion canine hind limbs during walking obtained with a 2-dimensional (2-D) system correlate well those 3-dimensional (3-D) and 2-D are repeatable. Animals —6 adult dogs no evidence lameness. Procedures —Hind limb motions 6 were recorded via video 3-D optoelectric systems simultaneously. Five valid trials digitized, 5 sets (2-D 60 Hz, 180 Hz) complete gait cycle created for each dog. In sets, reduced to exclude...

Abstract 3D cell printing technology is in the spotlight for producing tissue or organ constructs useful various medical applications. In of neuromuscular tissue, a bioink satisfying all requirements challenging issue. Gel integrity and motor neuron activity are two major characters because harmonious combination extracellular materials essential to consists disadvantages mechanical properties. Here, method fabrication presented using porcine central nervous system decellularized matrix...

Flow-based microfluidic biochips (FMBs) have seen rapid commercialization and deployment in recent years for point-of-care clinical diagnostics. However, the outsourcing of FMB design manufacturing makes them susceptible to malicious physical level intellectual property (IP)-theft attacks. This work demonstrates first structure-based (SB) attack on representative commercial FMBs. The SB attacks maliciously decrease heights reaction chambers produce false-negative results. We validate this...

Abstract The retinal pigment epithelium (RPE) not only forms the outer blood‐retinal barrier (oBRB) but also plays a multifunctional role in ocular system. loss of this leads to serious diseases resulting vision impairment. No effective treatment is available for repair RPE damage. A functional vitro model that allows recapitulation oBRB‐related pathophysiological responses lacking. Here, hybrid membrane printing technology developed fabricate cellular monolayers on basement mimic human...

Flow-based microfluidic biochips (FMBs) have been rapidly commercialized and deployed in recent years for biological computing, clinical diagnostics, point-of-care-tests (POCTs). However, outsourcing FMBs makes them susceptible to material-level attacks by malicious actors illegitimate monetary gain. The involve deliberate material degradation of an FMB's polydimethylsiloxane (PDMS) components either doping with reactive solvents or altering the PDMS curing ratio during fabrication. Such are...

Abstract In vitro organ models allow for the creation of precise preclinical that mimic physiology. During a pandemic life-threatening acute respiratory disease, an improved trachea model (TM) is required. We fabricated modular assembly blood vessel and TMs using 3D bioprinting technology. First, decellularized extracellular matrix (dECM) were prepared porcine vessels. A module was based on tracheal mucosa-derived dECM microporous membrane. Further, manufactured vascular-tissue-derived dECM....

A quartz crystal microbalance (QCM) technique is developed for the in situ analysis of cooling crystallization processes nucleation and growth. In contrast to conventional techniques based on property changes solid or solution phase, proposed QCM simultaneously exploits both phases, such as mass liquid viscosity, analyze processes. When initially solution, an increase viscosity reflected responses resonant frequency resistance. With further cooling, resistance sharply change at induction...

Conventional method of cell culture studies has been performed on two-dimensional substrates. Recently, three-dimensional (3D) platforms have a subject interest as cells in 3D significant differences differentiation and behavior. Here we report novel approach using nylon micro mesh (NMM) scaffold. NMM is commonly used laboratory, which eliminates the requirement special technicality for biological laboratories. Furthermore, it made micro-meter thick fibers, was adequate to engineer cellular...

A quartz crystal microbalance (QCM) is presented as a promising technique for determining the solubility and induction of nucleation via cooling reverse heating solution. When solution, resonant frequency (F) resistance (R) QCM responses change significantly due to vibrational loss related viscous elastic friction that depend on solution viscosity solid mass sensor, respectively. Thus, obvious refraction points appear in response profile at point primary during crystallization saturated...

Significance: Selective retina therapy (SRT) selectively targets the retinal pigment epithelium (RPE) and reduces negative side effects by avoiding thermal damages of adjacent photoreceptors, neural retina, choroid. However, selection proper laser energy for SRT is challenging because ophthalmoscopically invisible lesions in RPE different melanin concentrations among patients or even regions within an eye. Aim: We propose demonstrate monitoring based on speckle variance optical coherence...

Over the past few years, detection of biological analytes has intensively been studied to understand biodiagnostics various diseases [1]. Some that have used as biomarkers for specific include cell-free nucleic acids, (for example, DNA), messenger RNA (mRNA) and microRNA (miRNA) [2]-[5], circulating tumor-specific acids [circulating tumor DNA (ctDNA) ctRNA] [6]-[8], exosomes [9], [10], cells [11]. These biomolecules are known be effective indicators can diagnose presence a potential disease...

Retinal degeneration is a leading cause of incurable vision loss and blindness. The increasing incidence retinal has triggered research into the development in vitro models for drug alternatives transplantation. However, complex structure microenvironment pose serious challenges. Although 3D cell printing technology been widely used tissue engineering, including model regeneration medicine, currently available bioinks are insufficient to recapitulate extracellular matrix environment retina....

Self-assembly of silicon nanowire (SiNW) arrays is studied using SF6/O2 plasma treatment. The self-assembly method can be applied to single- and poly-crystalline Si substrates. Plasma conditions control the length diameter SiNW arrays. Lower reflectance wire over wavelength range 200–1100 nm obtained. conducting transparent indium-tin-oxide (ITO) electrode fully coated on self-assembled by sputtering. ITO-coated solar cells show same low surface light a higher carrier collection efficiency...