A5001165992- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Chemical Synthesis and Analysis
- Biopolymer Synthesis and Applications
- Herpesvirus Infections and Treatments
- Microbial Metabolites in Food Biotechnology
- Immunotherapy and Immune Responses
- Drug Transport and Resistance Mechanisms
- Helicobacter pylori-related gastroenterology studies
- Urticaria and Related Conditions
- COVID-19 Impact on Reproduction
- Immune responses and vaccinations
- Cancer Research and Treatments
- Neuropeptides and Animal Physiology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- vaccines and immunoinformatics approaches
- Viral Infections and Outbreaks Research
- Virus-based gene therapy research
Vitebsk State Medical University
2016
Russian Academy of Sciences
1991-2015
Institute of Bioorganic Chemistry
1980-2005
Both saponin and muramyl dipeptide (MDP) formulated with a squalane-in-water emulsion of large particle size prepared vortex mixer were superior to Al(OH)3 as adjuvants for HIV gp120 in mice. All the induced IgG1 antibody, but elicited highest titers IgG2a. The secretion interleukin-5 (IL-5) interferonγ (IFNγ) by lymph node cells cultured vitro was studied. cultures secreted IL-5, only those from saponin-immunized mice produced IFNγ, suggesting that is capable activating both Th1 TH2 T-cell...
Increased incidence of allergic diseases worldwide reflects some mangles the existing pharmacotherapy concept which ignores etiopathogenetic aspects clinical atopy. Meanwhile, understanding cellular and molecular mechanisms allergy may create prerequisites for development new therapeutic areas, in order to effectively influence pathogenesis points inflammation and, thus, leading success. The review article concerns an antagonism between two populations T-helper cells (Th1 Th2) carried out...
Abstract
Using flow cytometry and fluorescence polarization analysis, specific muramyl peptide‐binding sites were shown to be located inside T‐lymphocytes, macrophages neuroblastoma cells, but not B‐cells. No binding found on the cell surface. The number of for each type was determined. Two types observed myelomonocytic WEH1‐3 cells with K fd values 21 540 nM. Inhibition analysis demonstrated that effective binding, an intact glycopeptide molecule D‐configuration isoglutamine residue are important.
Summary Aspergillus fumigatus, a ubiquitous fungus, is implicated in the pathogenesis of number clinically different allergic diseases man, including broncopulmonary aspergillosis. Peptide-based immunotherapy may offer an alternative treatment strategy for management disease. The objective this study was to alter allergen-specific immune response using dominant T cell epitopes major A. fumigatus allergen, Asp f2, expressed yeast as virus-like particles (VLP). recognized mice with H-2d...
Disaccharide containing unit of peptidoglycan from bacterial cell wall, N-acetyl-d-glucosaminyl-N-acetylmuramyl-l-alanyl-d-glutaminamide (gluсosaminyl-muramyl-dipeptide) registered in Russia as an immunomodulatory drug, is shown to participate slow equilibrium α and β anomeric forms. Data NMR spectra molecular dynamics indicate that the α-anomer predominantly acquires a folded conformation stabilized by intramolecular hydrogen bond between alanyl carbonyl muramyl NH proton. The β-form...
The capability of immunoactive muramyl peptides to bind specifically rat brain membranes has been discovered. reaction an N ‐acetylglucosaminylmuramyl dipeptide analog having additional C‐terminal lysine residue (GMDP‐Lys) with Bolton‐Hunter reagent or ‐hydroxysuccinimidyl‐4‐azidosalicylate afforded two acylated derivatives, GMDP‐Lys‐(Hp) and GMDP‐Lys(Azs). Their iodination resulted in radioactive derivatives (spec. act. ∼2000 Ci/mmol) whose binding is characterized by K d ∼3 nM B max ∼10...
Objective. To study in vitro activity of antimicrobials against clinical isolates obtained from patients with community-acquired urinary tract infections (UTIs) various regions Russia 2023. Materials and Methods. The included 1098 the urine children adults both sexes all age groups acute exacerbation recurrent UTIs, including pregnant women asymptomatic bacteriuria. Isolates were collected 29 centers 21 cities 2023 as part a multicenter prospective epidemiological dynamics antibiotic...
By using radioligand analysis, murine peritoneal macrophages were shown to express several hundred high-affinity cell surface GMDP-binding sites (Ka 350 pM). Photoaffinity labeling followed by SDS-PAGE enabled us identify 32-34 and 38 kDa proteins inside these cells that bound GMDP specifically.
AbstractAbstractThe immunomodulatory effects of two synthetic muramyl peptides (MP): dipeptide and glucosaminyl- have been compared. It was shown, that MP on immune response are a consequence the alteration in T lymphocyte regulators balance. action old mice function stimulating only: increasing helper precursors frequency IL-1 production by macrophages. In latter both MPs acted as correctors, recovering decreased macrophages to young control level.Keywords: CM-culture mediumMP-muramyl...
Objective of the study. To evaluate pharmacological effects N-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-glutamic acid (GMDP-A) as a modifier biological reactions, i. e. to study its modifying action in regard traditional cytostatic chemotherapy. Materials and methods. The used drug agents were GMDP-A, cisplatinum, gemcitabine, cyclophosphamide, 5-fluorouracil. transplanted murine tumors Р-388 lymphocytic leukemia S-37 sarcoma (solid ascites variants), B-16 melanoma, CC-5 squamous cell...
Aim. To study the safety of preparation “Licopid®” using data, obtained with method questioning doctors various specialities. The reactant tablets is glucoseminylmuramildipeptide – a synthetic analogue structural fragment bacterial cells membrane (peptidoglycan), which an activator congenital and acquired immunity;it strengthens defense body from viral, fungal infections; it has adjuvant effect in development immunological reactions.
 Materials methods. object this were answers 2315...
Spread of allergic diseases testifies the inadequacy existing concept pharmacotherapy. The review discusses immunotherapy based on polarization immune response - activation Th1 and suppressing Th2-response. Clinical efficacy glucosaminylmuramyldipeptide in atopic asthma dermatitis is presented this clinical experimental preclinical studies data.
Abstract A pulse mode programmer has been developed for field desorption mass spectrometry that provides setting of the emitter heating current during scans, switching off intervals between consecutive scans and increasing it by 0.5 mA at start each scan. Using this device, temperature dependence spectra some peptide synthesis products studied.
During a pandemic, nonspecific immunoprophylaxis of SARS-CoV-2 infection and other acute respiratory infections (ARI), which can worsen the course COVID-19, is increasingly in demand addition to specific immunization. BCG vaccine appears be one candidate immunostimulants this regard. At same time, microbe-derived preparations capable inducing state trained immunity deserve attention. bacterial immunostimulatory agents containing large number biologically active subunits have long been...